Strain Fitness in Shewanella oneidensis MR-1 around SO3105

Experiment: 1-ethyl-3-methylimidazolium chloride 40 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO3103 and SO3104 are separated by 41 nucleotidesSO3104 and pspE-1 are separated by 25 nucleotidespspE-1 and aprE are separated by 576 nucleotides SO3103: SO3103 - AcrB/AcrD/AcrF family protein (NCBI ptt file), at 3,229,020 to 3,232,283 SO3103 SO3104: SO3104 - conserved hypothetical protein (NCBI ptt file), at 3,232,325 to 3,232,537 SO3104 SO3105: pspE-1 - phage shock protein E (NCBI ptt file), at 3,232,563 to 3,232,961 pspE-1 SO3106: aprE - cold-active serine alkaline protease (NCBI ptt file), at 3,233,538 to 3,235,961 aprE Position (kb) 3232 3233Strain fitness (log2 ratio) -2 -1 0 1 2at 3231.642 kb on + strand, within SO3103at 3231.650 kb on - strand, within SO3103at 3231.687 kb on + strand, within SO3103at 3231.809 kb on - strand, within SO3103at 3231.910 kb on - strand, within SO3103at 3232.410 kb on - strand, within SO3104at 3232.473 kb on - strand, within SO3104at 3232.729 kb on - strand, within pspE-1at 3232.767 kb on + strand, within pspE-1at 3232.922 kb on - strandat 3232.964 kb on - strandat 3233.056 kb on - strandat 3233.057 kb on + strandat 3233.581 kb on + strandat 3233.783 kb on - strand, within aprEat 3233.804 kb on - strand, within aprEat 3233.887 kb on + strand, within aprEat 3233.921 kb on - strand, within aprE

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 40 mM
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3,231,642 + SO3103 0.80 -0.2
3,231,650 - SO3103 0.81 +0.1
3,231,687 + SO3103 0.82 -0.4
3,231,809 - SO3103 0.85 +0.3
3,231,910 - SO3103 0.89 -1.8
3,232,410 - SO3104 0.40 -0.9
3,232,473 - SO3104 0.69 -0.1
3,232,729 - pspE-1 SO3105 0.42 -0.8
3,232,767 + pspE-1 SO3105 0.51 -0.9
3,232,922 - +0.6
3,232,964 - -0.6
3,233,056 - +1.9
3,233,057 + -0.7
3,233,581 + +0.2
3,233,783 - aprE SO3106 0.10 +0.3
3,233,804 - aprE SO3106 0.11 +1.3
3,233,887 + aprE SO3106 0.14 +0.4
3,233,921 - aprE SO3106 0.16 -0.1

Or see this region's nucleotide sequence