Strain Fitness in Shewanella oneidensis MR-1 around SO2731

Experiment: 1-ethyl-3-methylimidazolium chloride 40 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpepE and mdoG-2 are separated by 652 nucleotidesmdoG-2 and SO2732 are separated by 181 nucleotides SO2730: pepE - peptidase E (NCBI ptt file), at 2,845,799 to 2,846,509 pepE SO2731: mdoG-2 - periplasmic glucans biosynthesis protein MdoG (NCBI ptt file), at 2,847,162 to 2,848,748 mdoG-2 SO2732: SO2732 - ISSod6, transposase (NCBI ptt file), at 2,848,930 to 2,849,688 SO2732 Position (kb) 2847 2848 2849Strain fitness (log2 ratio) -3 -2 -1 0 1at 2846.169 kb on - strand, within pepEat 2846.225 kb on - strand, within pepEat 2846.240 kb on + strand, within pepEat 2846.248 kb on - strand, within pepEat 2846.375 kb on + strand, within pepEat 2846.383 kb on - strand, within pepEat 2846.383 kb on - strand, within pepEat 2846.393 kb on - strand, within pepEat 2846.401 kb on + strand, within pepEat 2846.409 kb on - strand, within pepEat 2846.477 kb on - strandat 2846.515 kb on + strandat 2846.523 kb on + strandat 2846.523 kb on - strandat 2846.940 kb on + strandat 2846.940 kb on + strandat 2846.940 kb on + strandat 2846.948 kb on - strandat 2846.948 kb on - strandat 2847.018 kb on - strandat 2847.178 kb on - strandat 2847.238 kb on + strandat 2847.279 kb on + strandat 2847.324 kb on + strand, within mdoG-2at 2847.395 kb on - strand, within mdoG-2at 2847.418 kb on - strand, within mdoG-2at 2847.557 kb on - strand, within mdoG-2at 2847.620 kb on + strand, within mdoG-2at 2847.660 kb on - strand, within mdoG-2at 2847.744 kb on + strand, within mdoG-2at 2847.752 kb on - strand, within mdoG-2at 2847.794 kb on + strand, within mdoG-2at 2847.794 kb on + strand, within mdoG-2at 2847.802 kb on - strand, within mdoG-2at 2847.802 kb on - strand, within mdoG-2at 2847.802 kb on - strand, within mdoG-2at 2847.807 kb on + strand, within mdoG-2at 2847.842 kb on + strand, within mdoG-2at 2848.029 kb on + strand, within mdoG-2at 2848.065 kb on + strand, within mdoG-2at 2848.076 kb on - strand, within mdoG-2at 2848.078 kb on - strand, within mdoG-2at 2848.100 kb on + strand, within mdoG-2at 2848.100 kb on + strand, within mdoG-2at 2848.181 kb on + strand, within mdoG-2at 2848.362 kb on + strand, within mdoG-2at 2848.370 kb on - strand, within mdoG-2at 2848.372 kb on + strand, within mdoG-2at 2848.390 kb on - strand, within mdoG-2at 2848.465 kb on + strand, within mdoG-2at 2848.571 kb on + strand, within mdoG-2at 2848.572 kb on + strand, within mdoG-2at 2848.618 kb on - strandat 2848.618 kb on - strandat 2848.745 kb on - strandat 2848.844 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 40 mM
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2,846,169 - pepE SO2730 0.52 +0.3
2,846,225 - pepE SO2730 0.60 -0.2
2,846,240 + pepE SO2730 0.62 -0.1
2,846,248 - pepE SO2730 0.63 -0.4
2,846,375 + pepE SO2730 0.81 +0.3
2,846,383 - pepE SO2730 0.82 -0.2
2,846,383 - pepE SO2730 0.82 -0.6
2,846,393 - pepE SO2730 0.84 +0.5
2,846,401 + pepE SO2730 0.85 -0.2
2,846,409 - pepE SO2730 0.86 +0.3
2,846,477 - -0.3
2,846,515 + +0.6
2,846,523 + -0.8
2,846,523 - -1.0
2,846,940 + -0.1
2,846,940 + -0.5
2,846,940 + -0.1
2,846,948 - -0.2
2,846,948 - -0.8
2,847,018 - -1.9
2,847,178 - -1.9
2,847,238 + -1.2
2,847,279 + -1.2
2,847,324 + mdoG-2 SO2731 0.10 -1.7
2,847,395 - mdoG-2 SO2731 0.15 -0.5
2,847,418 - mdoG-2 SO2731 0.16 -0.7
2,847,557 - mdoG-2 SO2731 0.25 -0.6
2,847,620 + mdoG-2 SO2731 0.29 +0.4
2,847,660 - mdoG-2 SO2731 0.31 -1.0
2,847,744 + mdoG-2 SO2731 0.37 -2.9
2,847,752 - mdoG-2 SO2731 0.37 -0.5
2,847,794 + mdoG-2 SO2731 0.40 -1.9
2,847,794 + mdoG-2 SO2731 0.40 -0.6
2,847,802 - mdoG-2 SO2731 0.40 +0.1
2,847,802 - mdoG-2 SO2731 0.40 -0.4
2,847,802 - mdoG-2 SO2731 0.40 -2.4
2,847,807 + mdoG-2 SO2731 0.41 -0.4
2,847,842 + mdoG-2 SO2731 0.43 -0.9
2,848,029 + mdoG-2 SO2731 0.55 -2.2
2,848,065 + mdoG-2 SO2731 0.57 -1.6
2,848,076 - mdoG-2 SO2731 0.58 -0.7
2,848,078 - mdoG-2 SO2731 0.58 -1.7
2,848,100 + mdoG-2 SO2731 0.59 -0.7
2,848,100 + mdoG-2 SO2731 0.59 -1.4
2,848,181 + mdoG-2 SO2731 0.64 -1.2
2,848,362 + mdoG-2 SO2731 0.76 -1.5
2,848,370 - mdoG-2 SO2731 0.76 -0.2
2,848,372 + mdoG-2 SO2731 0.76 -1.7
2,848,390 - mdoG-2 SO2731 0.77 -0.7
2,848,465 + mdoG-2 SO2731 0.82 -0.7
2,848,571 + mdoG-2 SO2731 0.89 -0.3
2,848,572 + mdoG-2 SO2731 0.89 -2.5
2,848,618 - -1.2
2,848,618 - -0.4
2,848,745 - -1.0
2,848,844 - -0.2

Or see this region's nucleotide sequence