Experiment: 1-ethyl-3-methylimidazolium chloride 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2156 and SO2157 are separated by 131 nucleotides SO2157 and SO2158 are separated by 56 nucleotides SO2158 and SO2159 are separated by 578 nucleotides
SO2156: SO2156 - alpha amylase family protein (NCBI ptt file), at 2,269,782 to 2,271,803
SO2156
SO2157: SO2157 - hypothetical protein (NCBI ptt file), at 2,271,935 to 2,272,171
SO2157
SO2158: SO2158 - hypothetical protein (NCBI ptt file), at 2,272,228 to 2,272,554
SO2158
SO2159: SO2159 - TonB domain protein (NCBI ptt file), at 2,273,133 to 2,273,432
SO2159
Position (kb)
2271
2272
2273 Strain fitness (log2 ratio)
-1
0
1 at 2271.011 kb on + strand, within SO2156 at 2271.092 kb on + strand, within SO2156 at 2271.099 kb on - strand, within SO2156 at 2271.100 kb on - strand, within SO2156 at 2271.100 kb on - strand, within SO2156 at 2271.269 kb on - strand, within SO2156 at 2271.284 kb on + strand, within SO2156 at 2271.284 kb on + strand, within SO2156 at 2271.292 kb on - strand, within SO2156 at 2271.292 kb on - strand, within SO2156 at 2271.292 kb on - strand, within SO2156 at 2271.454 kb on - strand, within SO2156 at 2271.500 kb on - strand, within SO2156 at 2271.594 kb on - strand, within SO2156 at 2271.649 kb on + strand at 2271.649 kb on + strand at 2271.650 kb on + strand at 2271.754 kb on + strand at 2271.825 kb on + strand at 2271.860 kb on + strand at 2271.865 kb on + strand at 2271.868 kb on - strand at 2271.980 kb on - strand, within SO2157 at 2272.045 kb on - strand, within SO2157 at 2272.060 kb on + strand, within SO2157 at 2272.092 kb on + strand, within SO2157 at 2272.100 kb on - strand, within SO2157 at 2272.220 kb on + strand at 2272.306 kb on + strand, within SO2158 at 2272.308 kb on + strand, within SO2158 at 2272.371 kb on - strand, within SO2158 at 2272.390 kb on - strand, within SO2158 at 2272.390 kb on - strand, within SO2158 at 2272.390 kb on - strand, within SO2158 at 2272.405 kb on + strand, within SO2158 at 2272.464 kb on + strand, within SO2158 at 2272.464 kb on + strand, within SO2158 at 2272.472 kb on - strand, within SO2158 at 2272.472 kb on - strand, within SO2158 at 2272.769 kb on + strand at 2272.777 kb on - strand at 2272.777 kb on - strand at 2272.865 kb on + strand at 2272.923 kb on - strand at 2272.943 kb on - strand at 2272.969 kb on - strand at 2273.000 kb on + strand at 2273.130 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 40 mM remove 2,271,011 + SO2156 0.61 -1.0 2,271,092 + SO2156 0.65 -0.8 2,271,099 - SO2156 0.65 -0.6 2,271,100 - SO2156 0.65 +0.2 2,271,100 - SO2156 0.65 -0.1 2,271,269 - SO2156 0.74 -0.6 2,271,284 + SO2156 0.74 +0.2 2,271,284 + SO2156 0.74 +0.1 2,271,292 - SO2156 0.75 +0.2 2,271,292 - SO2156 0.75 -0.5 2,271,292 - SO2156 0.75 +0.3 2,271,454 - SO2156 0.83 -1.2 2,271,500 - SO2156 0.85 +0.7 2,271,594 - SO2156 0.90 -0.7 2,271,649 + -0.0 2,271,649 + -0.1 2,271,650 + +0.2 2,271,754 + +0.3 2,271,825 + +0.1 2,271,860 + +1.1 2,271,865 + +0.8 2,271,868 - -1.4 2,271,980 - SO2157 0.19 -1.3 2,272,045 - SO2157 0.46 -0.3 2,272,060 + SO2157 0.53 -0.9 2,272,092 + SO2157 0.66 -0.6 2,272,100 - SO2157 0.70 -1.7 2,272,220 + -0.7 2,272,306 + SO2158 0.24 +0.0 2,272,308 + SO2158 0.24 -0.4 2,272,371 - SO2158 0.44 -0.1 2,272,390 - SO2158 0.50 -0.3 2,272,390 - SO2158 0.50 -0.2 2,272,390 - SO2158 0.50 +0.7 2,272,405 + SO2158 0.54 -0.0 2,272,464 + SO2158 0.72 -0.1 2,272,464 + SO2158 0.72 -0.1 2,272,472 - SO2158 0.75 +0.4 2,272,472 - SO2158 0.75 +0.1 2,272,769 + -0.2 2,272,777 - -0.9 2,272,777 - -0.5 2,272,865 + +0.2 2,272,923 - -0.0 2,272,943 - +1.5 2,272,969 - -0.7 2,273,000 + -0.3 2,273,130 + -1.5
Or see this region's nucleotide sequence