Experiment: 1-ethyl-3-methylimidazolium chloride 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aceA and SO1485 are separated by 312 nucleotides SO1485 and SO1486 are separated by 94 nucleotides SO1486 and SO1487 are separated by 54 nucleotides
SO1484: aceA - Isocitrate lyase (EC 4.1.3.1) (from data) , at 1,551,060 to 1,552,382
aceA
SO1485: SO1485 - hypothetical protein (NCBI ptt file), at 1,552,695 to 1,552,937
SO1485
SO1486: SO1486 - cytosolic long-chain acyl-CoA thioester hydrolase family protein (NCBI ptt file), at 1,553,032 to 1,553,400
SO1486
SO1487: SO1487 - hypothetical protein (NCBI ptt file), at 1,553,455 to 1,553,667
SO1487
Position (kb)
1552
1553 Strain fitness (log2 ratio)
-2
-1
0
1 at 1551.733 kb on + strand, within aceA at 1551.877 kb on - strand, within aceA at 1551.887 kb on - strand, within aceA at 1551.916 kb on - strand, within aceA at 1551.927 kb on + strand, within aceA at 1551.996 kb on + strand, within aceA at 1552.082 kb on + strand, within aceA at 1552.129 kb on - strand, within aceA at 1552.162 kb on + strand, within aceA at 1552.180 kb on + strand, within aceA at 1552.205 kb on - strand, within aceA at 1552.254 kb on + strand at 1552.269 kb on - strand at 1552.376 kb on + strand at 1552.384 kb on - strand at 1552.384 kb on - strand at 1552.440 kb on - strand at 1552.467 kb on + strand at 1552.527 kb on + strand at 1552.861 kb on + strand, within SO1485 at 1552.861 kb on + strand, within SO1485 at 1552.892 kb on - strand, within SO1485 at 1552.902 kb on - strand, within SO1485 at 1552.939 kb on - strand at 1552.940 kb on - strand at 1552.978 kb on + strand at 1552.986 kb on - strand at 1552.997 kb on + strand at 1553.055 kb on + strand at 1553.063 kb on - strand at 1553.063 kb on - strand at 1553.068 kb on - strand at 1553.117 kb on - strand, within SO1486 at 1553.204 kb on + strand, within SO1486 at 1553.252 kb on + strand, within SO1486 at 1553.252 kb on + strand, within SO1486 at 1553.252 kb on + strand, within SO1486 at 1553.252 kb on + strand, within SO1486 at 1553.260 kb on - strand, within SO1486 at 1553.287 kb on - strand, within SO1486 at 1553.295 kb on - strand, within SO1486 at 1553.339 kb on - strand, within SO1486 at 1553.428 kb on - strand at 1553.439 kb on + strand at 1553.619 kb on - strand, within SO1487 at 1553.632 kb on - strand, within SO1487 at 1553.642 kb on + strand, within SO1487 at 1553.643 kb on + strand, within SO1487 at 1553.747 kb on + strand at 1553.755 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 40 mM remove 1,551,733 + aceA SO1484 0.51 +0.6 1,551,877 - aceA SO1484 0.62 -1.1 1,551,887 - aceA SO1484 0.63 +0.1 1,551,916 - aceA SO1484 0.65 -0.1 1,551,927 + aceA SO1484 0.66 +0.3 1,551,996 + aceA SO1484 0.71 -0.1 1,552,082 + aceA SO1484 0.77 +0.0 1,552,129 - aceA SO1484 0.81 -0.3 1,552,162 + aceA SO1484 0.83 +0.0 1,552,180 + aceA SO1484 0.85 +0.6 1,552,205 - aceA SO1484 0.87 -0.1 1,552,254 + +0.3 1,552,269 - -0.5 1,552,376 + -0.8 1,552,384 - +0.3 1,552,384 - -0.0 1,552,440 - -0.2 1,552,467 + -0.8 1,552,527 + +0.2 1,552,861 + SO1485 0.68 -0.8 1,552,861 + SO1485 0.68 -0.6 1,552,892 - SO1485 0.81 -2.3 1,552,902 - SO1485 0.85 -0.2 1,552,939 - -1.9 1,552,940 - +0.1 1,552,978 + +0.1 1,552,986 - -0.8 1,552,997 + +0.1 1,553,055 + -0.1 1,553,063 - -0.0 1,553,063 - -0.2 1,553,068 - -0.5 1,553,117 - SO1486 0.23 +0.2 1,553,204 + SO1486 0.47 +0.1 1,553,252 + SO1486 0.60 +0.8 1,553,252 + SO1486 0.60 +0.2 1,553,252 + SO1486 0.60 -0.3 1,553,252 + SO1486 0.60 -1.1 1,553,260 - SO1486 0.62 -2.1 1,553,287 - SO1486 0.69 -0.8 1,553,295 - SO1486 0.71 +0.6 1,553,339 - SO1486 0.83 +0.2 1,553,428 - +0.6 1,553,439 + -1.2 1,553,619 - SO1487 0.77 -0.5 1,553,632 - SO1487 0.83 -0.4 1,553,642 + SO1487 0.88 +0.2 1,553,643 + SO1487 0.88 +1.1 1,553,747 + +0.5 1,553,755 - -0.3
Or see this region's nucleotide sequence