Strain Fitness in Shewanella oneidensis MR-1 around SO1475

Experiment: 1-ethyl-3-methylimidazolium chloride 40 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsmpB and SO1474 are separated by 264 nucleotidesSO1474 and SO1475 overlap by 11 nucleotidesSO1475 and smpA are separated by 91 nucleotides SO1473: smpB - SsrA-binding protein (NCBI ptt file), at 1,538,569 to 1,539,060 smpB SO1474: SO1474 - conserved hypothetical protein (NCBI ptt file), at 1,539,325 to 1,539,762 SO1474 SO1475: SO1475 - conserved hypothetical protein (NCBI ptt file), at 1,539,752 to 1,540,087 SO1475 SO1476: smpA - small protein A (NCBI ptt file), at 1,540,179 to 1,540,670 smpA Position (kb) 1539 1540 1541Strain fitness (log2 ratio) -1 0 1at 1539.101 kb on + strandat 1539.261 kb on + strandat 1539.261 kb on + strandat 1539.288 kb on + strandat 1540.039 kb on - strand, within SO1475at 1540.043 kb on + strand, within SO1475at 1540.043 kb on + strand, within SO1475at 1540.287 kb on - strand, within smpAat 1540.353 kb on - strand, within smpAat 1540.760 kb on + strandat 1540.767 kb on + strandat 1540.907 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 40 mM
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1,539,101 + +1.1
1,539,261 + +0.3
1,539,261 + -0.1
1,539,288 + +0.1
1,540,039 - SO1475 0.85 -0.6
1,540,043 + SO1475 0.87 -1.2
1,540,043 + SO1475 0.87 -0.9
1,540,287 - smpA SO1476 0.22 -1.2
1,540,353 - smpA SO1476 0.35 -0.1
1,540,760 + +0.2
1,540,767 + -0.2
1,540,907 - +0.0

Or see this region's nucleotide sequence