Experiment: 1-ethyl-3-methylimidazolium chloride 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hrpB and mrcB are separated by 77 nucleotides mrcB and ppiC-1 are separated by 77 nucleotides ppiC-1 and SO0636 are separated by 97 nucleotides SO0636 and SO0637 overlap by 4 nucleotides
SO0632: hrpB - ATP-dependent helicase HrpB (NCBI ptt file), at 658,076 to 660,583
hrpB
SO0633: mrcB - penicillin-binding protein 1B (NCBI ptt file), at 660,661 to 662,967
mrcB
SO0635: ppiC-1 - peptidyl-prolyl cis-trans isomerase C (NCBI ptt file), at 663,045 to 663,323
ppiC-1
SO0636: SO0636 - acetyltransferase, GNAT family (NCBI ptt file), at 663,421 to 663,915
SO0636
SO0637: SO0637 - conserved hypothetical protein (NCBI ptt file), at 663,912 to 664,187
SO0637
Position (kb) 660
661
662
663 Strain fitness (log2 ratio) -2
-1
0
1 at 659.697 kb on + strand, within hrpB at 659.705 kb on - strand, within hrpB at 659.729 kb on - strand, within hrpB at 659.776 kb on + strand, within hrpB at 659.841 kb on + strand, within hrpB at 659.841 kb on + strand, within hrpB at 659.849 kb on - strand, within hrpB at 659.849 kb on - strand, within hrpB at 659.849 kb on - strand, within hrpB at 659.851 kb on - strand, within hrpB at 659.859 kb on - strand, within hrpB at 660.060 kb on + strand, within hrpB at 660.068 kb on - strand, within hrpB at 660.068 kb on - strand, within hrpB at 660.104 kb on - strand, within hrpB at 660.249 kb on + strand, within hrpB at 660.249 kb on - strand, within hrpB at 660.273 kb on + strand, within hrpB at 660.281 kb on - strand, within hrpB at 660.289 kb on + strand, within hrpB at 660.336 kb on + strand at 660.336 kb on + strand at 660.376 kb on + strand at 660.383 kb on - strand at 660.395 kb on - strand at 660.402 kb on + strand at 660.405 kb on - strand at 660.457 kb on + strand at 660.495 kb on + strand at 660.533 kb on - strand at 660.584 kb on + strand at 660.584 kb on + strand at 660.584 kb on + strand at 660.592 kb on - strand at 660.592 kb on - strand at 660.592 kb on - strand at 660.619 kb on + strand at 660.627 kb on - strand at 660.746 kb on + strand at 660.746 kb on + strand at 660.754 kb on - strand at 660.793 kb on + strand at 660.799 kb on - strand at 660.884 kb on - strand at 660.917 kb on - strand, within mrcB at 660.972 kb on - strand, within mrcB at 661.037 kb on + strand, within mrcB at 661.078 kb on - strand, within mrcB at 661.274 kb on - strand, within mrcB at 661.324 kb on + strand, within mrcB at 661.332 kb on - strand, within mrcB at 661.334 kb on + strand, within mrcB at 661.342 kb on - strand, within mrcB at 661.488 kb on - strand, within mrcB at 661.629 kb on + strand, within mrcB at 661.629 kb on + strand, within mrcB at 661.637 kb on - strand, within mrcB at 661.641 kb on + strand, within mrcB at 661.646 kb on + strand, within mrcB at 661.646 kb on + strand, within mrcB at 661.646 kb on + strand, within mrcB at 661.646 kb on + strand, within mrcB at 661.646 kb on + strand, within mrcB at 661.646 kb on + strand, within mrcB at 661.646 kb on + strand, within mrcB at 661.646 kb on + strand, within mrcB at 661.654 kb on - strand, within mrcB at 661.654 kb on - strand, within mrcB at 661.695 kb on + strand, within mrcB at 661.695 kb on + strand, within mrcB at 661.701 kb on - strand, within mrcB at 661.703 kb on - strand, within mrcB at 661.703 kb on - strand, within mrcB at 661.703 kb on - strand, within mrcB at 661.710 kb on + strand, within mrcB at 661.731 kb on + strand, within mrcB at 661.731 kb on + strand, within mrcB at 661.739 kb on - strand, within mrcB at 661.823 kb on - strand, within mrcB at 661.823 kb on - strand, within mrcB at 662.041 kb on + strand, within mrcB at 662.185 kb on + strand, within mrcB at 662.234 kb on - strand, within mrcB at 662.324 kb on + strand, within mrcB at 662.410 kb on + strand, within mrcB at 662.436 kb on + strand, within mrcB at 662.465 kb on - strand, within mrcB at 662.475 kb on + strand, within mrcB at 662.565 kb on - strand, within mrcB at 662.611 kb on - strand, within mrcB at 662.663 kb on + strand, within mrcB at 662.673 kb on + strand, within mrcB at 662.803 kb on - strand at 662.868 kb on + strand at 662.903 kb on + strand at 663.090 kb on - strand, within ppiC-1 at 663.200 kb on + strand, within ppiC-1 at 663.432 kb on - strand at 663.475 kb on + strand, within SO0636 at 663.485 kb on - strand, within SO0636 at 663.519 kb on - strand, within SO0636 at 663.588 kb on + strand, within SO0636 at 663.732 kb on + strand, within SO0636 at 663.930 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 40 mM remove 659,697 + hrpB SO0632 0.65 +0.4 659,705 - hrpB SO0632 0.65 +0.1 659,729 - hrpB SO0632 0.66 -0.6 659,776 + hrpB SO0632 0.68 +0.3 659,841 + hrpB SO0632 0.70 +0.2 659,841 + hrpB SO0632 0.70 +0.3 659,849 - hrpB SO0632 0.71 -0.0 659,849 - hrpB SO0632 0.71 -0.4 659,849 - hrpB SO0632 0.71 -0.2 659,851 - hrpB SO0632 0.71 -0.6 659,859 - hrpB SO0632 0.71 -1.2 660,060 + hrpB SO0632 0.79 +0.2 660,068 - hrpB SO0632 0.79 -0.3 660,068 - hrpB SO0632 0.79 -1.8 660,104 - hrpB SO0632 0.81 -0.5 660,249 + hrpB SO0632 0.87 -1.2 660,249 - hrpB SO0632 0.87 +0.5 660,273 + hrpB SO0632 0.88 +0.7 660,281 - hrpB SO0632 0.88 +0.3 660,289 + hrpB SO0632 0.88 +0.7 660,336 + +0.7 660,336 + -0.3 660,376 + -0.2 660,383 - -0.1 660,395 - -0.8 660,402 + -0.0 660,405 - -0.6 660,457 + -0.3 660,495 + +0.2 660,533 - -0.8 660,584 + -1.1 660,584 + +0.6 660,584 + +0.3 660,592 - -2.2 660,592 - -2.2 660,592 - +0.2 660,619 + +0.4 660,627 - -0.7 660,746 + -0.0 660,746 + -0.5 660,754 - -0.3 660,793 + -1.2 660,799 - -1.1 660,884 - -1.4 660,917 - mrcB SO0633 0.11 +0.3 660,972 - mrcB SO0633 0.13 -1.3 661,037 + mrcB SO0633 0.16 -1.1 661,078 - mrcB SO0633 0.18 -1.2 661,274 - mrcB SO0633 0.27 -1.3 661,324 + mrcB SO0633 0.29 -1.4 661,332 - mrcB SO0633 0.29 -0.5 661,334 + mrcB SO0633 0.29 -0.4 661,342 - mrcB SO0633 0.30 -2.0 661,488 - mrcB SO0633 0.36 -0.9 661,629 + mrcB SO0633 0.42 -0.9 661,629 + mrcB SO0633 0.42 -0.0 661,637 - mrcB SO0633 0.42 -0.3 661,641 + mrcB SO0633 0.42 -1.1 661,646 + mrcB SO0633 0.43 -1.1 661,646 + mrcB SO0633 0.43 -0.1 661,646 + mrcB SO0633 0.43 +0.0 661,646 + mrcB SO0633 0.43 -0.3 661,646 + mrcB SO0633 0.43 -1.6 661,646 + mrcB SO0633 0.43 +0.1 661,646 + mrcB SO0633 0.43 -2.7 661,646 + mrcB SO0633 0.43 -1.6 661,654 - mrcB SO0633 0.43 -0.8 661,654 - mrcB SO0633 0.43 -1.1 661,695 + mrcB SO0633 0.45 -0.1 661,695 + mrcB SO0633 0.45 -0.3 661,701 - mrcB SO0633 0.45 -0.5 661,703 - mrcB SO0633 0.45 -1.4 661,703 - mrcB SO0633 0.45 -0.2 661,703 - mrcB SO0633 0.45 -0.9 661,710 + mrcB SO0633 0.45 -1.0 661,731 + mrcB SO0633 0.46 -1.7 661,731 + mrcB SO0633 0.46 -0.9 661,739 - mrcB SO0633 0.47 +0.1 661,823 - mrcB SO0633 0.50 -0.9 661,823 - mrcB SO0633 0.50 +0.1 662,041 + mrcB SO0633 0.60 -1.4 662,185 + mrcB SO0633 0.66 +0.5 662,234 - mrcB SO0633 0.68 -0.6 662,324 + mrcB SO0633 0.72 -1.1 662,410 + mrcB SO0633 0.76 -1.1 662,436 + mrcB SO0633 0.77 -1.2 662,465 - mrcB SO0633 0.78 +0.2 662,475 + mrcB SO0633 0.79 -1.1 662,565 - mrcB SO0633 0.83 +0.1 662,611 - mrcB SO0633 0.85 -2.0 662,663 + mrcB SO0633 0.87 -1.5 662,673 + mrcB SO0633 0.87 -1.0 662,803 - -0.9 662,868 + -0.8 662,903 + +0.5 663,090 - ppiC-1 SO0635 0.16 -0.4 663,200 + ppiC-1 SO0635 0.56 +0.2 663,432 - +0.2 663,475 + SO0636 0.11 +0.3 663,485 - SO0636 0.13 +0.6 663,519 - SO0636 0.20 -0.0 663,588 + SO0636 0.34 -0.7 663,732 + SO0636 0.63 -1.2 663,930 + -0.0
Or see this region's nucleotide sequence