Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3667 and SO3668 are separated by 33 nucleotides SO3668 and hugA are separated by 24 nucleotides hugA and tonB1 are separated by 245 nucleotides
SO3667: SO3667 - conserved hypothetical protein (NCBI ptt file), at 3,820,350 to 3,820,907
SO3667
SO3668: SO3668 - conserved hypothetical protein (NCBI ptt file), at 3,820,941 to 3,821,501
SO3668
SO3669: hugA - heme transport protein (NCBI ptt file), at 3,821,526 to 3,823,619
hugA
SO3670: tonB1 - TonB1 protein (NCBI ptt file), at 3,823,865 to 3,824,689
tonB1
Position (kb)
3821
3822
3823
3824 Strain fitness (log2 ratio)
-2
-1
0
1 at 3820.628 kb on - strand, within SO3667 at 3820.673 kb on - strand, within SO3667 at 3820.693 kb on - strand, within SO3667 at 3820.703 kb on + strand, within SO3667 at 3820.785 kb on + strand, within SO3667 at 3820.804 kb on + strand, within SO3667 at 3820.843 kb on - strand, within SO3667 at 3821.051 kb on - strand, within SO3668 at 3821.100 kb on + strand, within SO3668 at 3821.100 kb on + strand, within SO3668 at 3821.277 kb on - strand, within SO3668 at 3821.282 kb on - strand, within SO3668 at 3821.282 kb on - strand, within SO3668 at 3821.313 kb on - strand, within SO3668 at 3821.361 kb on + strand, within SO3668 at 3821.491 kb on + strand at 3821.524 kb on + strand at 3821.531 kb on - strand at 3821.550 kb on - strand at 3821.679 kb on - strand at 3821.690 kb on + strand at 3821.749 kb on + strand, within hugA at 3821.794 kb on + strand, within hugA at 3821.917 kb on + strand, within hugA at 3821.935 kb on - strand, within hugA at 3821.971 kb on + strand, within hugA at 3822.031 kb on - strand, within hugA at 3822.053 kb on + strand, within hugA at 3822.090 kb on - strand, within hugA at 3822.165 kb on + strand, within hugA at 3822.165 kb on + strand, within hugA at 3822.165 kb on + strand, within hugA at 3822.174 kb on - strand, within hugA at 3822.179 kb on - strand, within hugA at 3822.218 kb on - strand, within hugA at 3822.390 kb on + strand, within hugA at 3822.398 kb on - strand, within hugA at 3822.423 kb on - strand, within hugA at 3822.441 kb on + strand, within hugA at 3822.510 kb on - strand, within hugA at 3822.520 kb on + strand, within hugA at 3822.528 kb on - strand, within hugA at 3822.528 kb on - strand, within hugA at 3822.548 kb on - strand, within hugA at 3822.548 kb on - strand, within hugA at 3822.575 kb on + strand, within hugA at 3822.612 kb on + strand, within hugA at 3822.614 kb on + strand, within hugA at 3822.614 kb on + strand, within hugA at 3822.614 kb on + strand, within hugA at 3822.614 kb on + strand, within hugA at 3822.614 kb on + strand, within hugA at 3822.614 kb on + strand, within hugA at 3822.614 kb on + strand, within hugA at 3822.620 kb on - strand, within hugA at 3822.622 kb on - strand, within hugA at 3822.622 kb on - strand, within hugA at 3822.622 kb on - strand, within hugA at 3822.622 kb on - strand, within hugA at 3822.622 kb on - strand, within hugA at 3822.622 kb on - strand, within hugA at 3822.622 kb on - strand, within hugA at 3822.622 kb on - strand, within hugA at 3822.622 kb on - strand, within hugA at 3822.716 kb on + strand, within hugA at 3822.716 kb on + strand, within hugA at 3822.781 kb on - strand, within hugA at 3822.781 kb on - strand, within hugA at 3822.783 kb on + strand, within hugA at 3822.799 kb on + strand, within hugA at 3822.858 kb on + strand, within hugA at 3823.002 kb on + strand, within hugA at 3823.011 kb on - strand, within hugA at 3823.044 kb on + strand, within hugA at 3823.136 kb on + strand, within hugA at 3823.144 kb on - strand, within hugA at 3823.144 kb on - strand, within hugA at 3823.144 kb on - strand, within hugA at 3823.196 kb on + strand, within hugA at 3823.280 kb on + strand, within hugA at 3823.412 kb on + strand at 3823.437 kb on - strand at 3823.464 kb on + strand at 3823.464 kb on + strand at 3823.472 kb on - strand at 3823.472 kb on - strand at 3823.472 kb on - strand at 3823.505 kb on - strand at 3823.510 kb on - strand at 3823.520 kb on - strand at 3823.520 kb on - strand at 3823.561 kb on - strand at 3823.742 kb on + strand at 3823.921 kb on + strand at 3823.921 kb on + strand at 3823.929 kb on - strand at 3823.929 kb on - strand at 3823.936 kb on + strand at 3823.944 kb on - strand at 3823.944 kb on - strand at 3823.951 kb on + strand, within tonB1 at 3823.983 kb on - strand, within tonB1 at 3823.989 kb on - strand, within tonB1 at 3823.998 kb on - strand, within tonB1 at 3824.012 kb on - strand, within tonB1 at 3824.126 kb on + strand, within tonB1 at 3824.144 kb on - strand, within tonB1 at 3824.185 kb on + strand, within tonB1 at 3824.250 kb on + strand, within tonB1 at 3824.250 kb on + strand, within tonB1 at 3824.258 kb on - strand, within tonB1 at 3824.449 kb on + strand, within tonB1 at 3824.563 kb on - strand, within tonB1 at 3824.575 kb on - strand, within tonB1 at 3824.576 kb on - strand, within tonB1 at 3824.581 kb on + strand, within tonB1
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM remove 3,820,628 - SO3667 0.50 -0.2 3,820,673 - SO3667 0.58 -1.1 3,820,693 - SO3667 0.61 -2.5 3,820,703 + SO3667 0.63 -0.1 3,820,785 + SO3667 0.78 -0.1 3,820,804 + SO3667 0.81 -0.4 3,820,843 - SO3667 0.88 -0.3 3,821,051 - SO3668 0.20 +0.6 3,821,100 + SO3668 0.28 +0.2 3,821,100 + SO3668 0.28 +0.3 3,821,277 - SO3668 0.60 -0.2 3,821,282 - SO3668 0.61 -0.4 3,821,282 - SO3668 0.61 -1.4 3,821,313 - SO3668 0.66 +0.6 3,821,361 + SO3668 0.75 -1.0 3,821,491 + -1.3 3,821,524 + +0.3 3,821,531 - +0.6 3,821,550 - -0.7 3,821,679 - +0.7 3,821,690 + -0.8 3,821,749 + hugA SO3669 0.11 -0.2 3,821,794 + hugA SO3669 0.13 -1.7 3,821,917 + hugA SO3669 0.19 -0.4 3,821,935 - hugA SO3669 0.20 +0.0 3,821,971 + hugA SO3669 0.21 -0.7 3,822,031 - hugA SO3669 0.24 +1.2 3,822,053 + hugA SO3669 0.25 -1.2 3,822,090 - hugA SO3669 0.27 +0.3 3,822,165 + hugA SO3669 0.31 -0.3 3,822,165 + hugA SO3669 0.31 -0.3 3,822,165 + hugA SO3669 0.31 +0.4 3,822,174 - hugA SO3669 0.31 -0.3 3,822,179 - hugA SO3669 0.31 +0.9 3,822,218 - hugA SO3669 0.33 -0.7 3,822,390 + hugA SO3669 0.41 +0.0 3,822,398 - hugA SO3669 0.42 -0.7 3,822,423 - hugA SO3669 0.43 -0.6 3,822,441 + hugA SO3669 0.44 -0.9 3,822,510 - hugA SO3669 0.47 -0.6 3,822,520 + hugA SO3669 0.47 -0.3 3,822,528 - hugA SO3669 0.48 -0.5 3,822,528 - hugA SO3669 0.48 +0.3 3,822,548 - hugA SO3669 0.49 +0.2 3,822,548 - hugA SO3669 0.49 -0.2 3,822,575 + hugA SO3669 0.50 -0.4 3,822,612 + hugA SO3669 0.52 -0.6 3,822,614 + hugA SO3669 0.52 -1.0 3,822,614 + hugA SO3669 0.52 +0.0 3,822,614 + hugA SO3669 0.52 -0.2 3,822,614 + hugA SO3669 0.52 -0.5 3,822,614 + hugA SO3669 0.52 -0.9 3,822,614 + hugA SO3669 0.52 +0.6 3,822,614 + hugA SO3669 0.52 +0.3 3,822,620 - hugA SO3669 0.52 +0.4 3,822,622 - hugA SO3669 0.52 +0.4 3,822,622 - hugA SO3669 0.52 -0.8 3,822,622 - hugA SO3669 0.52 -0.5 3,822,622 - hugA SO3669 0.52 -0.7 3,822,622 - hugA SO3669 0.52 -0.4 3,822,622 - hugA SO3669 0.52 -0.2 3,822,622 - hugA SO3669 0.52 -0.4 3,822,622 - hugA SO3669 0.52 -0.2 3,822,622 - hugA SO3669 0.52 +1.5 3,822,716 + hugA SO3669 0.57 -1.1 3,822,716 + hugA SO3669 0.57 +0.1 3,822,781 - hugA SO3669 0.60 -0.6 3,822,781 - hugA SO3669 0.60 -1.2 3,822,783 + hugA SO3669 0.60 +0.1 3,822,799 + hugA SO3669 0.61 -1.9 3,822,858 + hugA SO3669 0.64 -0.6 3,823,002 + hugA SO3669 0.70 -0.9 3,823,011 - hugA SO3669 0.71 +0.8 3,823,044 + hugA SO3669 0.72 -1.5 3,823,136 + hugA SO3669 0.77 +0.5 3,823,144 - hugA SO3669 0.77 -1.5 3,823,144 - hugA SO3669 0.77 +0.2 3,823,144 - hugA SO3669 0.77 -0.4 3,823,196 + hugA SO3669 0.80 -0.1 3,823,280 + hugA SO3669 0.84 +1.0 3,823,412 + -0.1 3,823,437 - -0.8 3,823,464 + +0.5 3,823,464 + -0.1 3,823,472 - -0.2 3,823,472 - +0.9 3,823,472 - -0.2 3,823,505 - +0.4 3,823,510 - +0.5 3,823,520 - -0.6 3,823,520 - -0.8 3,823,561 - +0.3 3,823,742 + -0.7 3,823,921 + +0.2 3,823,921 + -0.3 3,823,929 - -0.7 3,823,929 - -0.2 3,823,936 + +0.2 3,823,944 - -0.6 3,823,944 - +0.4 3,823,951 + tonB1 SO3670 0.10 -0.1 3,823,983 - tonB1 SO3670 0.14 -0.6 3,823,989 - tonB1 SO3670 0.15 -1.8 3,823,998 - tonB1 SO3670 0.16 -1.0 3,824,012 - tonB1 SO3670 0.18 -0.8 3,824,126 + tonB1 SO3670 0.32 -0.7 3,824,144 - tonB1 SO3670 0.34 -1.6 3,824,185 + tonB1 SO3670 0.39 -2.2 3,824,250 + tonB1 SO3670 0.47 -0.7 3,824,250 + tonB1 SO3670 0.47 -0.2 3,824,258 - tonB1 SO3670 0.48 -1.0 3,824,449 + tonB1 SO3670 0.71 +0.0 3,824,563 - tonB1 SO3670 0.85 +0.3 3,824,575 - tonB1 SO3670 0.86 -1.4 3,824,576 - tonB1 SO3670 0.86 +0.2 3,824,581 + tonB1 SO3670 0.87 +0.5
Or see this region's nucleotide sequence