Experiment: 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rph and SO4257 are separated by 209 nucleotides SO4257 and SO4258 are separated by 182 nucleotides
SO4256: rph - ribonuclease PH (NCBI ptt file), at 4,432,337 to 4,433,050
rph
SO4257: SO4257 - conserved hypothetical protein TIGR00255 (NCBI ptt file), at 4,433,260 to 4,434,123
SO4257
SO4258: SO4258 - site-specific recombinase, phage integrase family (NCBI ptt file), at 4,434,306 to 4,435,187
SO4258
Position (kb)
4433
4434
4435 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4432.330 kb on + strand at 4432.503 kb on + strand, within rph at 4432.519 kb on - strand, within rph at 4432.553 kb on - strand, within rph at 4432.645 kb on - strand, within rph at 4432.661 kb on + strand, within rph at 4432.835 kb on + strand, within rph at 4432.836 kb on - strand, within rph at 4432.843 kb on - strand, within rph at 4432.843 kb on - strand, within rph at 4432.843 kb on - strand, within rph at 4432.843 kb on - strand, within rph at 4432.843 kb on - strand, within rph at 4432.886 kb on + strand, within rph at 4432.939 kb on + strand, within rph at 4432.974 kb on - strand, within rph at 4432.974 kb on - strand, within rph at 4432.982 kb on - strand at 4433.031 kb on + strand at 4433.067 kb on - strand at 4433.133 kb on - strand at 4433.169 kb on - strand at 4433.192 kb on + strand at 4433.199 kb on - strand at 4433.215 kb on + strand at 4433.251 kb on + strand at 4433.278 kb on + strand at 4433.354 kb on - strand, within SO4257 at 4433.409 kb on - strand, within SO4257 at 4433.570 kb on - strand, within SO4257 at 4433.571 kb on - strand, within SO4257 at 4433.594 kb on + strand, within SO4257 at 4433.597 kb on + strand, within SO4257 at 4433.602 kb on - strand, within SO4257 at 4433.602 kb on - strand, within SO4257 at 4433.607 kb on - strand, within SO4257 at 4433.607 kb on - strand, within SO4257 at 4433.607 kb on - strand, within SO4257 at 4433.615 kb on - strand, within SO4257 at 4433.617 kb on + strand, within SO4257 at 4433.697 kb on - strand, within SO4257 at 4433.697 kb on - strand, within SO4257 at 4433.718 kb on - strand, within SO4257 at 4433.733 kb on - strand, within SO4257 at 4433.744 kb on + strand, within SO4257 at 4433.802 kb on - strand, within SO4257 at 4433.832 kb on + strand, within SO4257 at 4433.838 kb on + strand, within SO4257 at 4433.846 kb on - strand, within SO4257 at 4433.849 kb on + strand, within SO4257 at 4433.920 kb on + strand, within SO4257 at 4433.931 kb on + strand, within SO4257 at 4434.023 kb on + strand, within SO4257 at 4434.031 kb on - strand, within SO4257 at 4434.105 kb on + strand at 4434.145 kb on + strand at 4434.229 kb on + strand at 4434.244 kb on - strand at 4434.244 kb on - strand at 4434.259 kb on - strand at 4434.340 kb on + strand at 4434.360 kb on - strand at 4434.361 kb on - strand at 4434.375 kb on + strand at 4434.387 kb on + strand at 4434.407 kb on - strand, within SO4258 at 4434.494 kb on + strand, within SO4258 at 4434.494 kb on + strand, within SO4258 at 4434.494 kb on + strand, within SO4258 at 4434.494 kb on + strand, within SO4258 at 4434.613 kb on + strand, within SO4258 at 4434.613 kb on + strand, within SO4258 at 4434.613 kb on + strand, within SO4258 at 4434.613 kb on + strand, within SO4258 at 4434.613 kb on + strand, within SO4258 at 4434.621 kb on - strand, within SO4258 at 4434.684 kb on + strand, within SO4258 at 4434.785 kb on - strand, within SO4258 at 4434.787 kb on + strand, within SO4258 at 4434.787 kb on + strand, within SO4258 at 4434.813 kb on + strand, within SO4258 at 4434.828 kb on - strand, within SO4258 at 4434.832 kb on + strand, within SO4258 at 4434.840 kb on - strand, within SO4258 at 4434.840 kb on - strand, within SO4258 at 4434.859 kb on + strand, within SO4258 at 4434.859 kb on + strand, within SO4258 at 4434.859 kb on + strand, within SO4258 at 4434.860 kb on + strand, within SO4258 at 4434.860 kb on + strand, within SO4258 at 4434.860 kb on + strand, within SO4258 at 4434.860 kb on + strand, within SO4258 at 4434.860 kb on + strand, within SO4258 at 4434.860 kb on + strand, within SO4258 at 4434.860 kb on + strand, within SO4258 at 4434.860 kb on + strand, within SO4258 at 4434.860 kb on + strand, within SO4258 at 4434.868 kb on - strand, within SO4258 at 4434.868 kb on - strand, within SO4258 at 4434.975 kb on + strand, within SO4258 at 4434.975 kb on + strand, within SO4258 at 4434.975 kb on + strand, within SO4258 at 4434.993 kb on + strand, within SO4258 at 4435.048 kb on - strand, within SO4258
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 20 mM remove 4,432,330 + +1.1 4,432,503 + rph SO4256 0.23 +0.8 4,432,519 - rph SO4256 0.25 -0.5 4,432,553 - rph SO4256 0.30 -1.2 4,432,645 - rph SO4256 0.43 -0.9 4,432,661 + rph SO4256 0.45 +0.7 4,432,835 + rph SO4256 0.70 -0.2 4,432,836 - rph SO4256 0.70 +0.0 4,432,843 - rph SO4256 0.71 -0.0 4,432,843 - rph SO4256 0.71 +0.1 4,432,843 - rph SO4256 0.71 +1.1 4,432,843 - rph SO4256 0.71 -0.4 4,432,843 - rph SO4256 0.71 -1.3 4,432,886 + rph SO4256 0.77 -2.9 4,432,939 + rph SO4256 0.84 -0.8 4,432,974 - rph SO4256 0.89 +0.4 4,432,974 - rph SO4256 0.89 +0.9 4,432,982 - +0.4 4,433,031 + +1.0 4,433,067 - +0.6 4,433,133 - +0.3 4,433,169 - -0.3 4,433,192 + +0.3 4,433,199 - -0.0 4,433,215 + +0.2 4,433,251 + +2.2 4,433,278 + -0.3 4,433,354 - SO4257 0.11 +1.0 4,433,409 - SO4257 0.17 -0.4 4,433,570 - SO4257 0.36 +1.1 4,433,571 - SO4257 0.36 +0.9 4,433,594 + SO4257 0.39 -0.5 4,433,597 + SO4257 0.39 -0.0 4,433,602 - SO4257 0.40 +0.2 4,433,602 - SO4257 0.40 -0.0 4,433,607 - SO4257 0.40 +1.5 4,433,607 - SO4257 0.40 +1.1 4,433,607 - SO4257 0.40 -0.5 4,433,615 - SO4257 0.41 +0.9 4,433,617 + SO4257 0.41 +1.0 4,433,697 - SO4257 0.51 +1.1 4,433,697 - SO4257 0.51 +0.5 4,433,718 - SO4257 0.53 +1.7 4,433,733 - SO4257 0.55 +0.4 4,433,744 + SO4257 0.56 +0.9 4,433,802 - SO4257 0.63 -0.4 4,433,832 + SO4257 0.66 -0.5 4,433,838 + SO4257 0.67 +0.1 4,433,846 - SO4257 0.68 +0.8 4,433,849 + SO4257 0.68 +0.7 4,433,920 + SO4257 0.76 +0.0 4,433,931 + SO4257 0.78 +1.3 4,434,023 + SO4257 0.88 +1.0 4,434,031 - SO4257 0.89 +2.2 4,434,105 + -1.7 4,434,145 + +0.1 4,434,229 + -2.1 4,434,244 - -0.6 4,434,244 - -0.0 4,434,259 - +0.4 4,434,340 + +0.1 4,434,360 - +0.2 4,434,361 - -0.6 4,434,375 + -1.2 4,434,387 + -0.4 4,434,407 - SO4258 0.11 +0.0 4,434,494 + SO4258 0.21 +0.6 4,434,494 + SO4258 0.21 +0.5 4,434,494 + SO4258 0.21 +1.1 4,434,494 + SO4258 0.21 +0.6 4,434,613 + SO4258 0.35 -0.3 4,434,613 + SO4258 0.35 +0.0 4,434,613 + SO4258 0.35 +0.2 4,434,613 + SO4258 0.35 +0.3 4,434,613 + SO4258 0.35 +1.8 4,434,621 - SO4258 0.36 +0.1 4,434,684 + SO4258 0.43 -0.2 4,434,785 - SO4258 0.54 -0.5 4,434,787 + SO4258 0.55 -0.1 4,434,787 + SO4258 0.55 -0.2 4,434,813 + SO4258 0.57 -0.2 4,434,828 - SO4258 0.59 +0.3 4,434,832 + SO4258 0.60 -2.2 4,434,840 - SO4258 0.61 -0.5 4,434,840 - SO4258 0.61 +1.9 4,434,859 + SO4258 0.63 +0.6 4,434,859 + SO4258 0.63 +0.2 4,434,859 + SO4258 0.63 +0.9 4,434,860 + SO4258 0.63 +0.5 4,434,860 + SO4258 0.63 -0.8 4,434,860 + SO4258 0.63 -0.1 4,434,860 + SO4258 0.63 -0.5 4,434,860 + SO4258 0.63 +0.2 4,434,860 + SO4258 0.63 -0.4 4,434,860 + SO4258 0.63 +0.5 4,434,860 + SO4258 0.63 +0.2 4,434,860 + SO4258 0.63 +0.3 4,434,868 - SO4258 0.64 +0.4 4,434,868 - SO4258 0.64 +0.2 4,434,975 + SO4258 0.76 +0.0 4,434,975 + SO4258 0.76 +0.6 4,434,975 + SO4258 0.76 -0.2 4,434,993 + SO4258 0.78 -0.1 4,435,048 - SO4258 0.84 -0.2
Or see this region's nucleotide sequence