Experiment: 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dfp and dut are separated by 0 nucleotides dut and SO4251 are separated by 31 nucleotides SO4251 and SO4252 are separated by 134 nucleotides
SO4249: dfp - DNA/pantothenate metabolism flavoprotein (NCBI ptt file), at 4,426,076 to 4,427,302
dfp
SO4250: dut - deoxyuridine 5'-triphosphate nucleotidohydrolase (NCBI ptt file), at 4,427,303 to 4,427,761
dut
SO4251: SO4251 - transcriptional regulator, TetR family (NCBI ptt file), at 4,427,793 to 4,428,386
SO4251
SO4252: SO4252 - prolyl oligopeptidase family protein (NCBI ptt file), at 4,428,521 to 4,430,509
SO4252
Position (kb)
4427
4428
4429 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4427.375 kb on + strand, within dut at 4427.514 kb on + strand, within dut at 4427.514 kb on + strand, within dut at 4427.514 kb on + strand, within dut at 4427.514 kb on + strand, within dut at 4427.514 kb on + strand, within dut at 4427.519 kb on + strand, within dut at 4427.522 kb on - strand, within dut at 4427.522 kb on - strand, within dut at 4427.522 kb on - strand, within dut at 4427.546 kb on - strand, within dut at 4427.622 kb on + strand, within dut at 4427.665 kb on - strand, within dut at 4427.791 kb on - strand at 4427.795 kb on + strand at 4427.840 kb on - strand at 4427.840 kb on - strand at 4427.863 kb on + strand, within SO4251 at 4427.908 kb on + strand, within SO4251 at 4427.993 kb on + strand, within SO4251 at 4428.036 kb on + strand, within SO4251 at 4428.057 kb on + strand, within SO4251 at 4428.232 kb on - strand, within SO4251 at 4428.252 kb on + strand, within SO4251 at 4428.338 kb on - strand at 4428.655 kb on - strand at 4428.777 kb on + strand, within SO4252 at 4428.795 kb on + strand, within SO4252 at 4428.803 kb on - strand, within SO4252 at 4428.805 kb on + strand, within SO4252 at 4428.837 kb on - strand, within SO4252 at 4428.871 kb on - strand, within SO4252 at 4428.985 kb on + strand, within SO4252 at 4429.036 kb on + strand, within SO4252 at 4429.036 kb on + strand, within SO4252 at 4429.039 kb on - strand, within SO4252 at 4429.044 kb on - strand, within SO4252 at 4429.044 kb on - strand, within SO4252 at 4429.104 kb on - strand, within SO4252 at 4429.151 kb on + strand, within SO4252 at 4429.189 kb on - strand, within SO4252 at 4429.200 kb on - strand, within SO4252 at 4429.229 kb on + strand, within SO4252 at 4429.255 kb on + strand, within SO4252 at 4429.313 kb on - strand, within SO4252 at 4429.342 kb on - strand, within SO4252
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 20 mM remove 4,427,375 + dut SO4250 0.16 +0.7 4,427,514 + dut SO4250 0.46 +1.7 4,427,514 + dut SO4250 0.46 +0.1 4,427,514 + dut SO4250 0.46 +0.6 4,427,514 + dut SO4250 0.46 +0.2 4,427,514 + dut SO4250 0.46 +1.2 4,427,519 + dut SO4250 0.47 +0.4 4,427,522 - dut SO4250 0.48 +2.1 4,427,522 - dut SO4250 0.48 +1.4 4,427,522 - dut SO4250 0.48 +0.6 4,427,546 - dut SO4250 0.53 +2.0 4,427,622 + dut SO4250 0.69 +1.1 4,427,665 - dut SO4250 0.79 +1.6 4,427,791 - +2.0 4,427,795 + +2.4 4,427,840 - +1.8 4,427,840 - +1.1 4,427,863 + SO4251 0.12 +1.2 4,427,908 + SO4251 0.19 +1.4 4,427,993 + SO4251 0.34 +1.1 4,428,036 + SO4251 0.41 +3.0 4,428,057 + SO4251 0.44 +1.6 4,428,232 - SO4251 0.74 +1.6 4,428,252 + SO4251 0.77 +1.7 4,428,338 - +1.6 4,428,655 - -0.1 4,428,777 + SO4252 0.13 -0.3 4,428,795 + SO4252 0.14 +0.5 4,428,803 - SO4252 0.14 -0.5 4,428,805 + SO4252 0.14 -0.1 4,428,837 - SO4252 0.16 -0.2 4,428,871 - SO4252 0.18 +0.0 4,428,985 + SO4252 0.23 -2.3 4,429,036 + SO4252 0.26 +0.3 4,429,036 + SO4252 0.26 +0.4 4,429,039 - SO4252 0.26 +0.8 4,429,044 - SO4252 0.26 +0.4 4,429,044 - SO4252 0.26 +0.6 4,429,104 - SO4252 0.29 -2.4 4,429,151 + SO4252 0.32 +0.3 4,429,189 - SO4252 0.34 -2.3 4,429,200 - SO4252 0.34 +0.1 4,429,229 + SO4252 0.36 +0.4 4,429,255 + SO4252 0.37 -0.8 4,429,313 - SO4252 0.40 -0.1 4,429,342 - SO4252 0.41 +0.2
Or see this region's nucleotide sequence