Experiment: 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hydB and SO3922 are separated by 13 nucleotides SO3922 and SO3923 are separated by 69 nucleotides SO3923 and SO3924 are separated by 0 nucleotides SO3924 and SO3925 overlap by 4 nucleotides
SO3921: hydB - periplasmic Fe hydrogenase, small subunit (NCBI ptt file), at 4,069,051 to 4,069,371
hydB
SO3922: SO3922 - formate dehydrogenase, putative (NCBI ptt file), at 4,069,385 to 4,070,068
SO3922
SO3923: SO3923 - thiH protein, putative (NCBI ptt file), at 4,070,138 to 4,071,577
SO3923
SO3924: SO3924 - hypothetical protein (NCBI ptt file), at 4,071,578 to 4,072,219
SO3924
SO3925: SO3925 - biotin synthase family protein (NCBI ptt file), at 4,072,216 to 4,073,295
SO3925
Position (kb)
4070
4071
4072 Strain fitness (log2 ratio)
-2
-1
0
1 at 4069.208 kb on + strand, within hydB at 4069.265 kb on - strand, within hydB at 4069.544 kb on + strand, within SO3922 at 4069.544 kb on - strand, within SO3922 at 4069.544 kb on - strand, within SO3922 at 4069.790 kb on + strand, within SO3922 at 4069.806 kb on + strand, within SO3922 at 4069.814 kb on - strand, within SO3922 at 4069.862 kb on + strand, within SO3922 at 4069.885 kb on - strand, within SO3922 at 4069.892 kb on - strand, within SO3922 at 4069.953 kb on - strand, within SO3922 at 4069.973 kb on - strand, within SO3922 at 4069.976 kb on - strand, within SO3922 at 4069.994 kb on + strand, within SO3922 at 4070.001 kb on + strand at 4070.001 kb on + strand at 4070.001 kb on + strand at 4070.002 kb on - strand at 4070.068 kb on + strand at 4070.076 kb on - strand at 4070.076 kb on - strand at 4070.089 kb on + strand at 4070.153 kb on - strand at 4070.160 kb on + strand at 4070.231 kb on - strand at 4070.231 kb on - strand at 4070.240 kb on + strand at 4070.248 kb on - strand at 4070.275 kb on - strand at 4070.297 kb on - strand, within SO3923 at 4070.302 kb on - strand, within SO3923 at 4070.302 kb on - strand, within SO3923 at 4070.309 kb on - strand, within SO3923 at 4070.588 kb on - strand, within SO3923 at 4070.637 kb on + strand, within SO3923 at 4070.637 kb on + strand, within SO3923 at 4070.637 kb on + strand, within SO3923 at 4070.639 kb on - strand, within SO3923 at 4070.645 kb on - strand, within SO3923 at 4070.645 kb on - strand, within SO3923 at 4070.683 kb on - strand, within SO3923 at 4070.716 kb on + strand, within SO3923 at 4070.775 kb on + strand, within SO3923 at 4070.836 kb on + strand, within SO3923 at 4070.883 kb on + strand, within SO3923 at 4070.891 kb on - strand, within SO3923 at 4070.891 kb on - strand, within SO3923 at 4070.891 kb on - strand, within SO3923 at 4070.997 kb on - strand, within SO3923 at 4071.059 kb on - strand, within SO3923 at 4071.088 kb on + strand, within SO3923 at 4071.088 kb on + strand, within SO3923 at 4071.088 kb on + strand, within SO3923 at 4071.096 kb on - strand, within SO3923 at 4071.096 kb on - strand, within SO3923 at 4071.096 kb on - strand, within SO3923 at 4071.098 kb on + strand, within SO3923 at 4071.098 kb on + strand, within SO3923 at 4071.099 kb on + strand, within SO3923 at 4071.316 kb on - strand, within SO3923 at 4071.493 kb on + strand at 4071.772 kb on + strand, within SO3924 at 4071.780 kb on - strand, within SO3924 at 4071.854 kb on - strand, within SO3924 at 4071.929 kb on + strand, within SO3924 at 4071.929 kb on + strand, within SO3924 at 4071.944 kb on + strand, within SO3924 at 4072.031 kb on + strand, within SO3924 at 4072.076 kb on - strand, within SO3924 at 4072.142 kb on - strand, within SO3924 at 4072.223 kb on - strand at 4072.278 kb on + strand at 4072.286 kb on - strand at 4072.296 kb on - strand at 4072.367 kb on - strand, within SO3925 at 4072.506 kb on + strand, within SO3925 at 4072.518 kb on + strand, within SO3925 at 4072.526 kb on - strand, within SO3925 at 4072.542 kb on + strand, within SO3925 at 4072.545 kb on - strand, within SO3925
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 20 mM remove 4,069,208 + hydB SO3921 0.49 +0.4 4,069,265 - hydB SO3921 0.67 +0.4 4,069,544 + SO3922 0.23 +1.0 4,069,544 - SO3922 0.23 +0.3 4,069,544 - SO3922 0.23 -0.2 4,069,790 + SO3922 0.59 -0.1 4,069,806 + SO3922 0.62 -0.7 4,069,814 - SO3922 0.63 +0.4 4,069,862 + SO3922 0.70 -1.6 4,069,885 - SO3922 0.73 +0.6 4,069,892 - SO3922 0.74 -0.1 4,069,953 - SO3922 0.83 +0.1 4,069,973 - SO3922 0.86 +0.7 4,069,976 - SO3922 0.86 -0.6 4,069,994 + SO3922 0.89 -1.1 4,070,001 + +1.6 4,070,001 + +0.2 4,070,001 + -0.4 4,070,002 - -1.1 4,070,068 + +0.4 4,070,076 - -0.1 4,070,076 - +0.6 4,070,089 + +0.8 4,070,153 - -0.3 4,070,160 + -0.2 4,070,231 - +0.2 4,070,231 - -1.2 4,070,240 + -0.1 4,070,248 - -0.6 4,070,275 - +0.7 4,070,297 - SO3923 0.11 +0.6 4,070,302 - SO3923 0.11 -0.2 4,070,302 - SO3923 0.11 -1.0 4,070,309 - SO3923 0.12 -0.1 4,070,588 - SO3923 0.31 +0.0 4,070,637 + SO3923 0.35 +0.2 4,070,637 + SO3923 0.35 -1.0 4,070,637 + SO3923 0.35 -0.5 4,070,639 - SO3923 0.35 +0.5 4,070,645 - SO3923 0.35 -0.2 4,070,645 - SO3923 0.35 +0.5 4,070,683 - SO3923 0.38 +0.6 4,070,716 + SO3923 0.40 -1.0 4,070,775 + SO3923 0.44 +1.2 4,070,836 + SO3923 0.48 +0.2 4,070,883 + SO3923 0.52 +1.6 4,070,891 - SO3923 0.52 +0.0 4,070,891 - SO3923 0.52 +0.8 4,070,891 - SO3923 0.52 +0.1 4,070,997 - SO3923 0.60 -0.6 4,071,059 - SO3923 0.64 +0.8 4,071,088 + SO3923 0.66 +0.9 4,071,088 + SO3923 0.66 -0.5 4,071,088 + SO3923 0.66 +0.1 4,071,096 - SO3923 0.67 +0.4 4,071,096 - SO3923 0.67 +0.2 4,071,096 - SO3923 0.67 +0.3 4,071,098 + SO3923 0.67 -0.5 4,071,098 + SO3923 0.67 +0.4 4,071,099 + SO3923 0.67 +0.8 4,071,316 - SO3923 0.82 +0.2 4,071,493 + -1.2 4,071,772 + SO3924 0.30 -0.8 4,071,780 - SO3924 0.31 -1.6 4,071,854 - SO3924 0.43 +0.0 4,071,929 + SO3924 0.55 +0.2 4,071,929 + SO3924 0.55 +0.9 4,071,944 + SO3924 0.57 -0.3 4,072,031 + SO3924 0.71 -0.1 4,072,076 - SO3924 0.78 +0.4 4,072,142 - SO3924 0.88 -0.4 4,072,223 - -0.3 4,072,278 + -0.1 4,072,286 - +1.1 4,072,296 - -0.2 4,072,367 - SO3925 0.14 +0.6 4,072,506 + SO3925 0.27 -0.3 4,072,518 + SO3925 0.28 +0.8 4,072,526 - SO3925 0.29 -1.8 4,072,542 + SO3925 0.30 +0.3 4,072,545 - SO3925 0.30 +0.7
Or see this region's nucleotide sequence