Experiment: 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purL and SO3288 are separated by 343 nucleotides SO3288 and SO3291 are separated by 459 nucleotides
SO3287: purL - phosphoribosylformylglycinamidine synthase (NCBI ptt file), at 3,428,937 to 3,432,818
purL
SO3288: SO3288 - transglycosylase, Slt family (NCBI ptt file), at 3,433,162 to 3,434,535
SO3288
SO3291: SO3291 - cytidine/deoxycytidylate deaminase family protein (NCBI ptt file), at 3,434,995 to 3,435,579
SO3291
Position (kb)
3433
3434
3435 Strain fitness (log2 ratio)
-1
0
1
2 at 3432.189 kb on - strand, within purL at 3432.307 kb on - strand, within purL at 3432.335 kb on + strand, within purL at 3432.335 kb on + strand, within purL at 3432.343 kb on - strand, within purL at 3432.345 kb on + strand, within purL at 3432.367 kb on + strand, within purL at 3432.487 kb on - strand at 3432.708 kb on + strand at 3432.721 kb on - strand at 3432.799 kb on - strand at 3432.861 kb on - strand at 3432.861 kb on - strand at 3432.861 kb on - strand at 3432.861 kb on - strand at 3432.861 kb on - strand at 3432.905 kb on - strand at 3432.920 kb on + strand at 3433.005 kb on + strand at 3433.015 kb on + strand at 3433.160 kb on - strand at 3433.241 kb on + strand at 3433.249 kb on - strand at 3433.292 kb on - strand at 3433.344 kb on + strand, within SO3288 at 3433.390 kb on + strand, within SO3288 at 3433.544 kb on - strand, within SO3288 at 3433.591 kb on - strand, within SO3288 at 3433.606 kb on + strand, within SO3288 at 3433.654 kb on + strand, within SO3288 at 3433.677 kb on - strand, within SO3288 at 3433.679 kb on - strand, within SO3288 at 3433.788 kb on - strand, within SO3288 at 3434.075 kb on - strand, within SO3288 at 3434.240 kb on - strand, within SO3288 at 3434.268 kb on + strand, within SO3288 at 3434.508 kb on - strand at 3434.510 kb on + strand at 3434.510 kb on + strand at 3434.510 kb on + strand at 3434.510 kb on + strand at 3434.809 kb on - strand at 3434.809 kb on - strand at 3434.809 kb on - strand at 3434.950 kb on + strand at 3435.011 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 20 mM remove 3,432,189 - purL SO3287 0.84 +2.0 3,432,307 - purL SO3287 0.87 +1.7 3,432,335 + purL SO3287 0.88 -0.1 3,432,335 + purL SO3287 0.88 -0.0 3,432,343 - purL SO3287 0.88 +0.9 3,432,345 + purL SO3287 0.88 +0.2 3,432,367 + purL SO3287 0.88 -0.2 3,432,487 - -0.4 3,432,708 + +0.2 3,432,721 - +0.4 3,432,799 - +0.2 3,432,861 - -0.5 3,432,861 - -0.9 3,432,861 - +1.0 3,432,861 - +0.5 3,432,861 - +0.2 3,432,905 - +0.8 3,432,920 + +0.1 3,433,005 + -1.0 3,433,015 + +0.6 3,433,160 - +1.7 3,433,241 + +1.6 3,433,249 - +2.3 3,433,292 - +2.4 3,433,344 + SO3288 0.13 +2.0 3,433,390 + SO3288 0.17 +2.4 3,433,544 - SO3288 0.28 +1.2 3,433,591 - SO3288 0.31 +0.7 3,433,606 + SO3288 0.32 +1.2 3,433,654 + SO3288 0.36 +1.7 3,433,677 - SO3288 0.37 +1.6 3,433,679 - SO3288 0.38 +2.2 3,433,788 - SO3288 0.46 +2.1 3,434,075 - SO3288 0.66 +2.0 3,434,240 - SO3288 0.78 +1.7 3,434,268 + SO3288 0.80 +2.1 3,434,508 - +1.7 3,434,510 + +0.9 3,434,510 + -1.4 3,434,510 + +1.3 3,434,510 + -0.4 3,434,809 - -0.4 3,434,809 - +0.5 3,434,809 - -0.1 3,434,950 + +0.9 3,435,011 + -0.0
Or see this region's nucleotide sequence