Experiment: 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1183 and pth are separated by 168 nucleotides pth and SO1185 are separated by 154 nucleotides SO1185 and tRNA-Met-8 are separated by 207 nucleotides tRNA-Met-8 and tRNA-Leu-9 are separated by 54 nucleotides tRNA-Leu-9 and tRNA-Gln-3 are separated by 46 nucleotides tRNA-Gln-3 and tRNA-Gln-2 are separated by 21 nucleotides tRNA-Gln-2 and tRNA-Met-7 are separated by 1 nucleotides tRNA-Met-7 and tRNA-Leu-8 are separated by 81 nucleotides tRNA-Leu-8 and tRNA-Gln-1 are separated by 36 nucleotides
SO1183: SO1183 - oxidoreductase, FAD-binding (NCBI ptt file), at 1,226,116 to 1,227,330
SO1183
SO1184: pth - peptidyl-tRNA hydrolase (NCBI ptt file), at 1,227,499 to 1,228,086
pth
SO1185: SO1185 - conserved hypothetical protein TIGR00092 (NCBI ptt file), at 1,228,241 to 1,229,332
SO1185
SO_t014: tRNA-Met-8 - tRNA-Met (RefSeq), at 1,229,540 to 1,229,616
tRNA-Met-8
SO_t015: tRNA-Leu-9 - tRNA-Leu (RefSeq), at 1,229,671 to 1,229,755
tRNA-Leu-9
SO_t016: tRNA-Gln-3 - tRNA-Gln (RefSeq), at 1,229,802 to 1,229,876
tRNA-Gln-3
SO_t017: tRNA-Gln-2 - tRNA-Gln (RefSeq), at 1,229,898 to 1,229,972
tRNA-Gln-2
SO_t018: tRNA-Met-7 - tRNA-Met (RefSeq), at 1,229,974 to 1,230,050
tRNA-Met-7
SO_t019: tRNA-Leu-8 - tRNA-Leu (RefSeq), at 1,230,132 to 1,230,216
tRNA-Leu-8
SO_t020: tRNA-Gln-1 - tRNA-Gln (RefSeq), at 1,230,253 to 1,230,327
tRNA-Gln-1
Position (kb)
1228
1229
1230 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1227.398 kb on + strand at 1227.474 kb on + strand at 1227.474 kb on + strand at 1228.070 kb on + strand at 1228.080 kb on + strand at 1228.102 kb on + strand at 1228.120 kb on - strand at 1228.167 kb on + strand at 1228.167 kb on + strand at 1228.167 kb on + strand at 1228.175 kb on - strand at 1228.178 kb on + strand at 1228.268 kb on + strand at 1228.292 kb on + strand at 1228.306 kb on + strand at 1228.432 kb on + strand, within SO1185 at 1228.565 kb on - strand, within SO1185 at 1228.663 kb on - strand, within SO1185 at 1228.725 kb on - strand, within SO1185 at 1228.841 kb on - strand, within SO1185 at 1228.894 kb on + strand, within SO1185 at 1228.906 kb on - strand, within SO1185 at 1228.988 kb on + strand, within SO1185 at 1229.019 kb on + strand, within SO1185 at 1229.033 kb on + strand, within SO1185 at 1229.033 kb on + strand, within SO1185 at 1229.033 kb on + strand, within SO1185 at 1229.041 kb on - strand, within SO1185 at 1229.041 kb on - strand, within SO1185 at 1229.041 kb on - strand, within SO1185 at 1229.041 kb on - strand, within SO1185 at 1229.057 kb on + strand, within SO1185 at 1229.123 kb on + strand, within SO1185 at 1229.131 kb on - strand, within SO1185 at 1229.131 kb on - strand, within SO1185 at 1229.131 kb on - strand, within SO1185 at 1229.281 kb on + strand at 1229.306 kb on + strand at 1229.346 kb on + strand at 1229.413 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 20 mM remove 1,227,398 + -0.1 1,227,474 + +0.1 1,227,474 + -0.9 1,228,070 + -0.8 1,228,080 + +0.5 1,228,102 + +0.1 1,228,120 - -1.6 1,228,167 + +0.1 1,228,167 + -1.3 1,228,167 + +1.0 1,228,175 - -1.6 1,228,178 + -0.1 1,228,268 + -2.3 1,228,292 + -3.4 1,228,306 + -1.5 1,228,432 + SO1185 0.17 -1.6 1,228,565 - SO1185 0.30 -1.2 1,228,663 - SO1185 0.39 -1.1 1,228,725 - SO1185 0.44 +0.2 1,228,841 - SO1185 0.55 -1.3 1,228,894 + SO1185 0.60 -1.5 1,228,906 - SO1185 0.61 -2.3 1,228,988 + SO1185 0.68 -2.8 1,229,019 + SO1185 0.71 -3.0 1,229,033 + SO1185 0.73 -1.4 1,229,033 + SO1185 0.73 -2.2 1,229,033 + SO1185 0.73 -0.7 1,229,041 - SO1185 0.73 -2.4 1,229,041 - SO1185 0.73 -1.4 1,229,041 - SO1185 0.73 -2.0 1,229,041 - SO1185 0.73 -0.8 1,229,057 + SO1185 0.75 -0.6 1,229,123 + SO1185 0.81 -0.2 1,229,131 - SO1185 0.82 -4.3 1,229,131 - SO1185 0.82 -3.9 1,229,131 - SO1185 0.82 -0.7 1,229,281 + -2.2 1,229,306 + -2.4 1,229,346 + +0.1 1,229,413 + +0.6
Or see this region's nucleotide sequence