Strain Fitness in Shewanella oneidensis MR-1 around SO1185

Experiment: 1-ethyl-3-methylimidazolium acetate 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO1183 and pth are separated by 168 nucleotidespth and SO1185 are separated by 154 nucleotidesSO1185 and tRNA-Met-8 are separated by 207 nucleotidestRNA-Met-8 and tRNA-Leu-9 are separated by 54 nucleotidestRNA-Leu-9 and tRNA-Gln-3 are separated by 46 nucleotidestRNA-Gln-3 and tRNA-Gln-2 are separated by 21 nucleotidestRNA-Gln-2 and tRNA-Met-7 are separated by 1 nucleotidestRNA-Met-7 and tRNA-Leu-8 are separated by 81 nucleotidestRNA-Leu-8 and tRNA-Gln-1 are separated by 36 nucleotides SO1183: SO1183 - oxidoreductase, FAD-binding (NCBI ptt file), at 1,226,116 to 1,227,330 SO1183 SO1184: pth - peptidyl-tRNA hydrolase (NCBI ptt file), at 1,227,499 to 1,228,086 pth SO1185: SO1185 - conserved hypothetical protein TIGR00092 (NCBI ptt file), at 1,228,241 to 1,229,332 SO1185 SO_t014: tRNA-Met-8 - tRNA-Met (RefSeq), at 1,229,540 to 1,229,616 tRNA-Met-8 SO_t015: tRNA-Leu-9 - tRNA-Leu (RefSeq), at 1,229,671 to 1,229,755 tRNA-Leu-9 SO_t016: tRNA-Gln-3 - tRNA-Gln (RefSeq), at 1,229,802 to 1,229,876 tRNA-Gln-3 SO_t017: tRNA-Gln-2 - tRNA-Gln (RefSeq), at 1,229,898 to 1,229,972 tRNA-Gln-2 SO_t018: tRNA-Met-7 - tRNA-Met (RefSeq), at 1,229,974 to 1,230,050 tRNA-Met-7 SO_t019: tRNA-Leu-8 - tRNA-Leu (RefSeq), at 1,230,132 to 1,230,216 tRNA-Leu-8 SO_t020: tRNA-Gln-1 - tRNA-Gln (RefSeq), at 1,230,253 to 1,230,327 tRNA-Gln-1 Position (kb) 1228 1229 1230Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1227.398 kb on + strandat 1227.474 kb on + strandat 1227.474 kb on + strandat 1228.070 kb on + strandat 1228.080 kb on + strandat 1228.102 kb on + strandat 1228.120 kb on - strandat 1228.167 kb on + strandat 1228.167 kb on + strandat 1228.167 kb on + strandat 1228.175 kb on - strandat 1228.178 kb on + strandat 1228.268 kb on + strandat 1228.292 kb on + strandat 1228.306 kb on + strandat 1228.432 kb on + strand, within SO1185at 1228.565 kb on - strand, within SO1185at 1228.663 kb on - strand, within SO1185at 1228.725 kb on - strand, within SO1185at 1228.841 kb on - strand, within SO1185at 1228.894 kb on + strand, within SO1185at 1228.906 kb on - strand, within SO1185at 1228.988 kb on + strand, within SO1185at 1229.019 kb on + strand, within SO1185at 1229.033 kb on + strand, within SO1185at 1229.033 kb on + strand, within SO1185at 1229.033 kb on + strand, within SO1185at 1229.041 kb on - strand, within SO1185at 1229.041 kb on - strand, within SO1185at 1229.041 kb on - strand, within SO1185at 1229.041 kb on - strand, within SO1185at 1229.057 kb on + strand, within SO1185at 1229.123 kb on + strand, within SO1185at 1229.131 kb on - strand, within SO1185at 1229.131 kb on - strand, within SO1185at 1229.131 kb on - strand, within SO1185at 1229.281 kb on + strandat 1229.306 kb on + strandat 1229.346 kb on + strandat 1229.413 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 20 mM
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1,227,398 + -0.1
1,227,474 + +0.1
1,227,474 + -0.9
1,228,070 + -0.8
1,228,080 + +0.5
1,228,102 + +0.1
1,228,120 - -1.6
1,228,167 + +0.1
1,228,167 + -1.3
1,228,167 + +1.0
1,228,175 - -1.6
1,228,178 + -0.1
1,228,268 + -2.3
1,228,292 + -3.4
1,228,306 + -1.5
1,228,432 + SO1185 0.17 -1.6
1,228,565 - SO1185 0.30 -1.2
1,228,663 - SO1185 0.39 -1.1
1,228,725 - SO1185 0.44 +0.2
1,228,841 - SO1185 0.55 -1.3
1,228,894 + SO1185 0.60 -1.5
1,228,906 - SO1185 0.61 -2.3
1,228,988 + SO1185 0.68 -2.8
1,229,019 + SO1185 0.71 -3.0
1,229,033 + SO1185 0.73 -1.4
1,229,033 + SO1185 0.73 -2.2
1,229,033 + SO1185 0.73 -0.7
1,229,041 - SO1185 0.73 -2.4
1,229,041 - SO1185 0.73 -1.4
1,229,041 - SO1185 0.73 -2.0
1,229,041 - SO1185 0.73 -0.8
1,229,057 + SO1185 0.75 -0.6
1,229,123 + SO1185 0.81 -0.2
1,229,131 - SO1185 0.82 -4.3
1,229,131 - SO1185 0.82 -3.9
1,229,131 - SO1185 0.82 -0.7
1,229,281 + -2.2
1,229,306 + -2.4
1,229,346 + +0.1
1,229,413 + +0.6

Or see this region's nucleotide sequence