Experiment: 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0701 and SO0702 are separated by 3 nucleotides SO0702 and groES are separated by 227 nucleotides groES and groEL are separated by 58 nucleotides
SO0701: SO0701 - transcriptional regulator, LysR family (NCBI ptt file), at 718,091 to 719,080
SO0701
SO0702: SO0702 - MATE efflux family protein (NCBI ptt file), at 719,084 to 720,442
SO0702
SO0703: groES - chaperonin GroES (NCBI ptt file), at 720,670 to 720,960
groES
SO0704: groEL - chaperonin GroEL (NCBI ptt file), at 721,019 to 722,656
groEL
Position (kb)
719
720
721 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 718.216 kb on + strand, within SO0701 at 718.310 kb on - strand, within SO0701 at 718.357 kb on - strand, within SO0701 at 718.403 kb on - strand, within SO0701 at 718.403 kb on - strand, within SO0701 at 718.433 kb on + strand, within SO0701 at 718.441 kb on - strand, within SO0701 at 718.452 kb on + strand, within SO0701 at 718.470 kb on - strand, within SO0701 at 718.696 kb on + strand, within SO0701 at 718.823 kb on + strand, within SO0701 at 719.008 kb on - strand at 719.014 kb on + strand at 719.031 kb on + strand at 719.032 kb on + strand at 719.177 kb on - strand at 719.177 kb on - strand at 719.240 kb on + strand, within SO0702 at 719.260 kb on + strand, within SO0702 at 719.276 kb on + strand, within SO0702 at 719.386 kb on - strand, within SO0702 at 719.410 kb on - strand, within SO0702 at 719.853 kb on + strand, within SO0702 at 719.934 kb on + strand, within SO0702 at 720.000 kb on - strand, within SO0702 at 720.050 kb on - strand, within SO0702 at 720.136 kb on + strand, within SO0702 at 720.208 kb on + strand, within SO0702 at 720.216 kb on - strand, within SO0702 at 720.307 kb on - strand at 720.348 kb on - strand at 720.382 kb on + strand at 720.387 kb on + strand at 720.390 kb on - strand at 720.392 kb on + strand at 720.395 kb on - strand at 720.397 kb on + strand at 720.419 kb on + strand at 720.432 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 20 mM remove 718,216 + SO0701 0.13 +3.7 718,310 - SO0701 0.22 +3.3 718,357 - SO0701 0.27 +2.8 718,403 - SO0701 0.32 +2.3 718,403 - SO0701 0.32 +2.6 718,433 + SO0701 0.35 +3.6 718,441 - SO0701 0.35 +2.4 718,452 + SO0701 0.36 +2.6 718,470 - SO0701 0.38 +2.4 718,696 + SO0701 0.61 +2.7 718,823 + SO0701 0.74 +1.6 719,008 - +2.3 719,014 + +0.7 719,031 + -0.2 719,032 + +1.3 719,177 - -2.6 719,177 - -0.6 719,240 + SO0702 0.11 -0.6 719,260 + SO0702 0.13 -1.8 719,276 + SO0702 0.14 -2.0 719,386 - SO0702 0.22 -2.1 719,410 - SO0702 0.24 -2.7 719,853 + SO0702 0.57 -1.0 719,934 + SO0702 0.63 -0.5 720,000 - SO0702 0.67 -3.8 720,050 - SO0702 0.71 -1.9 720,136 + SO0702 0.77 -1.2 720,208 + SO0702 0.83 -2.3 720,216 - SO0702 0.83 -2.2 720,307 - -2.3 720,348 - -3.4 720,382 + -0.7 720,387 + -2.9 720,390 - -1.7 720,392 + -1.7 720,395 - -3.0 720,397 + -2.2 720,419 + -3.1 720,432 - -2.9
Or see this region's nucleotide sequence