Experiment: 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dam and SO0290 are separated by 32 nucleotides SO0290 and SO0291 overlap by 4 nucleotides SO0291 and rpe are separated by 262 nucleotides rpe and gph overlap by 1 nucleotides
SO0289: dam - DNA adenine methylase (NCBI ptt file), at 296,921 to 297,760
dam
SO0290: SO0290 - hypothetical protein (NCBI ptt file), at 297,793 to 297,981
SO0290
SO0291: SO0291 - hypothetical protein (NCBI ptt file), at 297,978 to 298,343
SO0291
SO0292: rpe - ribulose-phosphate 3-epimerase (NCBI ptt file), at 298,606 to 299,283
rpe
SO0293: gph - phosphoglycolate phosphatase (NCBI ptt file), at 299,283 to 299,966
gph
Position (kb) 297
298
299 Strain fitness (log2 ratio) -2
-1
0
1
2 at 297.924 kb on + strand, within SO0290 at 297.957 kb on - strand, within SO0290 at 297.997 kb on - strand at 298.029 kb on - strand, within SO0291 at 298.044 kb on - strand, within SO0291 at 298.054 kb on - strand, within SO0291 at 298.178 kb on - strand, within SO0291 at 298.187 kb on - strand, within SO0291 at 298.232 kb on - strand, within SO0291 at 298.500 kb on + strand at 298.540 kb on + strand at 299.000 kb on + strand, within rpe
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 20 mM remove 297,924 + SO0290 0.69 +0.5 297,957 - SO0290 0.87 -1.6 297,997 - +0.4 298,029 - SO0291 0.14 -0.2 298,044 - SO0291 0.18 +2.4 298,054 - SO0291 0.21 +1.8 298,178 - SO0291 0.55 +2.2 298,187 - SO0291 0.57 +1.4 298,232 - SO0291 0.69 +1.5 298,500 + -2.4 298,540 + -1.3 299,000 + rpe SO0292 0.58 -2.5
Or see this region's nucleotide sequence