Experiment: 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0081 and SO0082 are separated by 3 nucleotides SO0082 and SO0083 are separated by 187 nucleotides SO0083 and SO0084 are separated by 57 nucleotides SO0084 and SO0085 are separated by 375 nucleotides
SO0081: SO0081 - conserved hypothetical protein (NCBI ptt file), at 93,827 to 94,156
SO0081
SO0082: SO0082 - transcriptional regulator, MerR family (NCBI ptt file), at 94,160 to 94,516
SO0082
SO0083: SO0083 - carboxymuconolactone decarboxylase family protein (NCBI ptt file), at 94,704 to 95,090
SO0083
SO0084: SO0084 - HAD-superfamily hydrolase, subfamily IA, variant 1 family protein (NCBI ptt file), at 95,148 to 95,828
SO0084
SO0085: SO0085 - hypothetical protein (NCBI ptt file), at 96,204 to 96,626
SO0085
Position (kb) 95
96 Strain fitness (log2 ratio) -2
-1
0
1 at 94.186 kb on + strand at 94.304 kb on - strand, within SO0082 at 94.364 kb on - strand, within SO0082 at 94.377 kb on - strand, within SO0082 at 94.434 kb on - strand, within SO0082 at 94.451 kb on + strand, within SO0082 at 94.597 kb on + strand at 94.605 kb on - strand at 94.654 kb on + strand at 94.663 kb on - strand at 94.669 kb on - strand at 94.669 kb on - strand at 94.670 kb on - strand at 94.722 kb on + strand at 94.740 kb on - strand at 94.811 kb on - strand, within SO0083 at 94.839 kb on - strand, within SO0083 at 94.891 kb on - strand, within SO0083 at 94.934 kb on - strand, within SO0083 at 94.938 kb on + strand, within SO0083 at 95.316 kb on + strand, within SO0084 at 95.471 kb on + strand, within SO0084 at 95.517 kb on + strand, within SO0084 at 95.541 kb on + strand, within SO0084 at 95.549 kb on - strand, within SO0084 at 95.575 kb on + strand, within SO0084 at 95.575 kb on + strand, within SO0084 at 95.583 kb on - strand, within SO0084 at 95.583 kb on - strand, within SO0084 at 95.583 kb on - strand, within SO0084 at 95.583 kb on - strand, within SO0084 at 95.583 kb on - strand, within SO0084 at 95.606 kb on - strand, within SO0084 at 95.637 kb on + strand, within SO0084 at 95.642 kb on + strand, within SO0084 at 95.645 kb on - strand, within SO0084 at 95.782 kb on - strand at 95.802 kb on + strand at 95.802 kb on + strand at 95.810 kb on - strand at 95.832 kb on - strand at 95.870 kb on + strand at 95.914 kb on + strand at 95.926 kb on + strand at 95.934 kb on - strand at 95.935 kb on - strand at 95.942 kb on + strand at 96.051 kb on - strand at 96.066 kb on - strand at 96.085 kb on - strand at 96.150 kb on + strand at 96.171 kb on + strand at 96.179 kb on - strand at 96.186 kb on + strand at 96.186 kb on + strand at 96.187 kb on - strand at 96.275 kb on + strand, within SO0085 at 96.280 kb on - strand, within SO0085 at 96.285 kb on + strand, within SO0085 at 96.428 kb on + strand, within SO0085 at 96.442 kb on + strand, within SO0085 at 96.451 kb on - strand, within SO0085 at 96.451 kb on - strand, within SO0085 at 96.451 kb on - strand, within SO0085 at 96.493 kb on - strand, within SO0085 at 96.533 kb on + strand, within SO0085 at 96.534 kb on + strand, within SO0085 at 96.549 kb on - strand, within SO0085 at 96.556 kb on + strand, within SO0085 at 96.603 kb on + strand at 96.604 kb on + strand at 96.604 kb on + strand at 96.604 kb on + strand at 96.612 kb on - strand at 96.612 kb on - strand at 96.614 kb on + strand at 96.624 kb on - strand at 96.657 kb on + strand at 96.657 kb on + strand at 96.657 kb on + strand at 96.665 kb on - strand at 96.665 kb on - strand at 96.665 kb on - strand at 96.756 kb on - strand at 96.758 kb on - strand at 96.768 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 20 mM remove 94,186 + +1.1 94,304 - SO0082 0.40 -0.2 94,364 - SO0082 0.57 -0.3 94,377 - SO0082 0.61 +0.5 94,434 - SO0082 0.77 +0.8 94,451 + SO0082 0.82 +0.4 94,597 + -0.1 94,605 - +0.9 94,654 + -0.8 94,663 - +0.3 94,669 - +0.0 94,669 - +0.1 94,670 - +0.4 94,722 + +0.2 94,740 - -0.4 94,811 - SO0083 0.28 +0.2 94,839 - SO0083 0.35 +0.6 94,891 - SO0083 0.48 -0.5 94,934 - SO0083 0.59 -0.4 94,938 + SO0083 0.60 -0.0 95,316 + SO0084 0.25 -1.1 95,471 + SO0084 0.47 -2.0 95,517 + SO0084 0.54 -2.2 95,541 + SO0084 0.58 -0.3 95,549 - SO0084 0.59 -1.0 95,575 + SO0084 0.63 -0.5 95,575 + SO0084 0.63 -1.9 95,583 - SO0084 0.64 -1.4 95,583 - SO0084 0.64 -1.7 95,583 - SO0084 0.64 -1.5 95,583 - SO0084 0.64 +0.8 95,583 - SO0084 0.64 -1.8 95,606 - SO0084 0.67 -1.2 95,637 + SO0084 0.72 -1.6 95,642 + SO0084 0.73 -1.0 95,645 - SO0084 0.73 -1.2 95,782 - +0.1 95,802 + -1.8 95,802 + -1.4 95,810 - -1.0 95,832 - +0.3 95,870 + -1.8 95,914 + +0.2 95,926 + -0.5 95,934 - +0.7 95,935 - -0.2 95,942 + -0.1 96,051 - -0.5 96,066 - +0.7 96,085 - -0.1 96,150 + -0.2 96,171 + +0.5 96,179 - -0.1 96,186 + -0.4 96,186 + -1.0 96,187 - +0.1 96,275 + SO0085 0.17 -1.6 96,280 - SO0085 0.18 -0.5 96,285 + SO0085 0.19 -0.0 96,428 + SO0085 0.53 +0.0 96,442 + SO0085 0.56 -0.9 96,451 - SO0085 0.58 -0.7 96,451 - SO0085 0.58 -0.7 96,451 - SO0085 0.58 -0.5 96,493 - SO0085 0.68 +0.4 96,533 + SO0085 0.78 +0.2 96,534 + SO0085 0.78 +0.8 96,549 - SO0085 0.82 +0.5 96,556 + SO0085 0.83 -0.3 96,603 + -1.0 96,604 + +0.7 96,604 + +0.3 96,604 + -1.3 96,612 - +0.6 96,612 - +0.5 96,614 + -0.3 96,624 - +0.7 96,657 + +0.4 96,657 + -0.2 96,657 + +0.3 96,665 - +0.7 96,665 - +0.3 96,665 - +0.3 96,756 - +0.6 96,758 - +0.5 96,768 + -1.4
Or see this region's nucleotide sequence