Experiment: acetate 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt secF-1 and SO1195 are separated by 56 nucleotides SO1195 and rrmJ are separated by 90 nucleotides rrmJ and ftsH are separated by 53 nucleotides
SO1194: secF-1 - protein-export membrane protein SecF (NCBI ptt file), at 1,237,851 to 1,238,735
secF-1
SO1195: SO1195 - conserved hypothetical protein TIGR00253 (NCBI ptt file), at 1,238,792 to 1,239,091
SO1195
SO1196: rrmJ - ribosomal RNA large subunit methyltransferase J (NCBI ptt file), at 1,239,182 to 1,239,811
rrmJ
SO1197: ftsH - cell division protein FtsH (NCBI ptt file), at 1,239,865 to 1,241,814
ftsH
Position (kb)
1239
1240 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1238.199 kb on - strand, within secF-1 at 1238.227 kb on + strand, within secF-1 at 1238.237 kb on - strand, within secF-1 at 1238.245 kb on - strand, within secF-1 at 1238.402 kb on - strand, within secF-1 at 1238.550 kb on + strand, within secF-1 at 1238.603 kb on - strand, within secF-1 at 1238.611 kb on - strand, within secF-1 at 1238.976 kb on + strand, within SO1195 at 1239.139 kb on - strand at 1239.139 kb on - strand at 1239.141 kb on + strand at 1239.343 kb on - strand, within rrmJ at 1239.660 kb on + strand, within rrmJ at 1239.771 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction acetate 50 mM remove 1,238,199 - secF-1 SO1194 0.39 +0.0 1,238,227 + secF-1 SO1194 0.42 -0.2 1,238,237 - secF-1 SO1194 0.44 -0.2 1,238,245 - secF-1 SO1194 0.45 +0.2 1,238,402 - secF-1 SO1194 0.62 +0.2 1,238,550 + secF-1 SO1194 0.79 +0.1 1,238,603 - secF-1 SO1194 0.85 +0.1 1,238,611 - secF-1 SO1194 0.86 -0.4 1,238,976 + SO1195 0.61 -1.0 1,239,139 - -2.8 1,239,139 - +0.9 1,239,141 + -1.0 1,239,343 - rrmJ SO1196 0.26 -2.1 1,239,660 + rrmJ SO1196 0.76 -1.6 1,239,771 + -2.5
Or see this region's nucleotide sequence