Experiment: 7H9 with 2,4-Dinitrophenol 0.1503 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0050 and Rv0051 overlap by 4 nucleotides Rv0051 and Rv0052 are separated by 31 nucleotides Rv0052 and Rv0053 are separated by 218 nucleotides
Rv0050: Rv0050 - Probable bifunctional penicillin-binding protein 1A/1B PonA1 (murein polymerase) (PBP1): penicillin-insensitive transglycosylase (peptidoglycan TGASE) + penicillin-sensitive transpeptidase (DD-transpeptidase), at 53,663 to 55,699
Rv0050
Rv0051: Rv0051 - Probable conserved transmembrane protein, at 55,696 to 57,378
Rv0051
Rv0052: Rv0052 - Conserved protein, at 57,410 to 57,973
Rv0052
Rv0053: Rv0053 - 30S ribosomal protein S6 RpsF, at 58,192 to 58,482
Rv0053
Position (kb) 55
56
57
58 Strain fitness (log2 ratio) -2
-1
0
1 at 54.759 kb on + strand, within Rv0050 at 54.870 kb on - strand, within Rv0050 at 55.086 kb on - strand, within Rv0050 at 55.252 kb on - strand, within Rv0050 at 55.560 kb on + strand at 55.796 kb on + strand at 56.008 kb on + strand, within Rv0051 at 56.155 kb on + strand, within Rv0051 at 56.157 kb on + strand, within Rv0051 at 56.186 kb on - strand, within Rv0051 at 56.229 kb on - strand, within Rv0051 at 56.521 kb on + strand, within Rv0051 at 56.540 kb on - strand, within Rv0051 at 57.046 kb on + strand, within Rv0051 at 57.047 kb on - strand, within Rv0051 at 57.050 kb on + strand, within Rv0051 at 57.051 kb on - strand, within Rv0051 at 57.052 kb on + strand, within Rv0051 at 57.510 kb on + strand, within Rv0052 at 57.510 kb on + strand, within Rv0052 at 57.702 kb on + strand, within Rv0052 at 57.879 kb on - strand, within Rv0052 at 57.957 kb on - strand at 58.105 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with 2,4-Dinitrophenol 0.1503 mM remove 54,759 + Rv0050 0.54 -0.0 54,870 - Rv0050 0.59 +0.8 55,086 - Rv0050 0.70 -0.8 55,252 - Rv0050 0.78 -1.8 55,560 + -0.4 55,796 + -1.3 56,008 + Rv0051 0.19 -0.6 56,155 + Rv0051 0.27 -1.8 56,157 + Rv0051 0.27 -0.2 56,186 - Rv0051 0.29 -1.0 56,229 - Rv0051 0.32 -0.8 56,521 + Rv0051 0.49 -0.0 56,540 - Rv0051 0.50 -0.2 57,046 + Rv0051 0.80 -1.9 57,047 - Rv0051 0.80 -0.9 57,050 + Rv0051 0.80 -1.3 57,051 - Rv0051 0.81 +0.5 57,052 + Rv0051 0.81 -0.2 57,510 + Rv0052 0.18 -0.6 57,510 + Rv0052 0.18 +0.3 57,702 + Rv0052 0.52 -0.1 57,879 - Rv0052 0.83 -1.7 57,957 - +0.0 58,105 + -0.2
Or see this region's nucleotide sequence