Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2446c

Experiment: 7H9 with Phenazine ethosulfate 0.0137 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2444c and Rv2445c are separated by 329 nucleotidesRv2445c and Rv2446c are separated by 42 nucleotidesRv2446c and Rv2447c overlap by 4 nucleotides Rv2444c: Rv2444c - Possible ribonuclease E Rne, at 2,742,123 to 2,744,984 Rv2444c Rv2445c: Rv2445c - Probable nucleoside diphosphate kinase NdkA (NDK) (NDP kinase) (nucleoside-2-P kinase), at 2,745,314 to 2,745,724 Rv2445c Rv2446c: Rv2446c - Probable conserved integral membrane protein, at 2,745,767 to 2,746,138 Rv2446c Rv2447c: Rv2447c - Probable folylpolyglutamate synthase protein FolC (folylpoly-gamma-glutamate synthetase) (FPGS), at 2,746,135 to 2,747,598 Rv2447c Position (kb) 2745 2746 2747Strain fitness (log2 ratio) -2 -1 0 1at 2744.980 kb on - strandat 2744.980 kb on - strandat 2745.044 kb on - strandat 2745.737 kb on + strandat 2745.737 kb on + strandat 2745.737 kb on + strandat 2745.737 kb on + strandat 2745.737 kb on + strandat 2745.737 kb on + strandat 2745.738 kb on - strandat 2745.738 kb on - strandat 2745.753 kb on + strandat 2745.753 kb on + strandat 2745.753 kb on + strandat 2745.753 kb on + strandat 2745.754 kb on - strandat 2745.754 kb on - strandat 2745.754 kb on - strandat 2745.816 kb on + strand, within Rv2446cat 2745.816 kb on + strand, within Rv2446cat 2745.817 kb on - strand, within Rv2446cat 2745.960 kb on - strand, within Rv2446cat 2745.960 kb on - strand, within Rv2446cat 2745.960 kb on - strand, within Rv2446cat 2745.988 kb on - strand, within Rv2446c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Phenazine ethosulfate 0.0137 mM
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2,744,980 - -1.0
2,744,980 - -1.5
2,745,044 - -0.3
2,745,737 + -0.9
2,745,737 + +0.0
2,745,737 + -0.9
2,745,737 + +0.2
2,745,737 + -0.2
2,745,737 + -1.8
2,745,738 - +1.0
2,745,738 - -0.0
2,745,753 + -0.6
2,745,753 + -0.6
2,745,753 + -0.5
2,745,753 + +1.7
2,745,754 - -0.2
2,745,754 - +1.2
2,745,754 - +0.2
2,745,816 + Rv2446c 0.13 -1.5
2,745,816 + Rv2446c 0.13 -0.2
2,745,817 - Rv2446c 0.13 -1.2
2,745,960 - Rv2446c 0.52 +0.7
2,745,960 - Rv2446c 0.52 -0.3
2,745,960 - Rv2446c 0.52 -1.6
2,745,988 - Rv2446c 0.59 -0.9

Or see this region's nucleotide sequence