Experiment: 7H9 with Phenazine ethosulfate 0.0137 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0184 and Rv0185 overlap by 4 nucleotides Rv0185 and Rv0186 are separated by 44 nucleotides Rv0186 and Rv0186A are separated by 45 nucleotides Rv0186A and Rv0187 are separated by 153 nucleotides
Rv0184: Rv0184 - Conserved hypothetical protein, at 214,969 to 215,718
Rv0184
Rv0185: Rv0185 - Conserved hypothetical protein, at 215,715 to 216,224
Rv0185
Rv0186: Rv0186 - Probable beta-glucosidase BglS (gentiobiase) (cellobiase) (beta-D-glucoside glucohydrolase), at 216,269 to 218,344
Rv0186
Rv0186A: Rv0186A - Metallothionein, MymT, at 218,390 to 218,551
Rv0186A
Rv0187: Rv0187 - Probable O-methyltransferase, at 218,705 to 219,367
Rv0187
Position (kb)
216
217
218
219 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 215.318 kb on + strand, within Rv0184 at 215.319 kb on - strand, within Rv0184 at 215.319 kb on - strand, within Rv0184 at 215.320 kb on + strand, within Rv0184 at 215.761 kb on - strand at 215.895 kb on + strand, within Rv0185 at 215.895 kb on + strand, within Rv0185 at 215.896 kb on - strand, within Rv0185 at 216.020 kb on + strand, within Rv0185 at 216.021 kb on - strand, within Rv0185 at 216.076 kb on + strand, within Rv0185 at 216.076 kb on + strand, within Rv0185 at 216.202 kb on - strand at 216.378 kb on - strand at 216.378 kb on - strand at 216.869 kb on + strand, within Rv0186 at 217.101 kb on + strand, within Rv0186 at 217.298 kb on - strand, within Rv0186 at 217.343 kb on + strand, within Rv0186 at 217.988 kb on + strand, within Rv0186 at 217.989 kb on - strand, within Rv0186 at 218.099 kb on + strand, within Rv0186 at 218.100 kb on - strand, within Rv0186 at 218.100 kb on - strand, within Rv0186 at 218.100 kb on - strand, within Rv0186 at 218.100 kb on - strand, within Rv0186 at 218.102 kb on - strand, within Rv0186 at 218.385 kb on + strand at 218.385 kb on + strand at 218.386 kb on - strand at 218.386 kb on - strand at 218.386 kb on - strand at 218.596 kb on + strand at 218.610 kb on + strand at 218.614 kb on + strand at 218.615 kb on - strand at 218.820 kb on + strand, within Rv0187 at 218.981 kb on + strand, within Rv0187 at 218.981 kb on + strand, within Rv0187 at 218.982 kb on - strand, within Rv0187 at 219.148 kb on + strand, within Rv0187 at 219.205 kb on + strand, within Rv0187 at 219.206 kb on - strand, within Rv0187 at 219.298 kb on - strand, within Rv0187
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Phenazine ethosulfate 0.0137 mM remove 215,318 + Rv0184 0.47 -0.6 215,319 - Rv0184 0.47 +2.8 215,319 - Rv0184 0.47 -1.2 215,320 + Rv0184 0.47 -0.8 215,761 - -0.1 215,895 + Rv0185 0.35 -0.6 215,895 + Rv0185 0.35 +0.2 215,896 - Rv0185 0.35 -0.4 216,020 + Rv0185 0.60 -0.1 216,021 - Rv0185 0.60 -0.7 216,076 + Rv0185 0.71 -0.4 216,076 + Rv0185 0.71 -0.0 216,202 - +0.6 216,378 - -0.2 216,378 - +0.2 216,869 + Rv0186 0.29 -1.6 217,101 + Rv0186 0.40 -0.7 217,298 - Rv0186 0.50 -2.3 217,343 + Rv0186 0.52 -1.1 217,988 + Rv0186 0.83 -1.2 217,989 - Rv0186 0.83 -0.1 218,099 + Rv0186 0.88 -0.1 218,100 - Rv0186 0.88 +0.2 218,100 - Rv0186 0.88 -0.3 218,100 - Rv0186 0.88 -0.0 218,100 - Rv0186 0.88 +0.6 218,102 - Rv0186 0.88 -2.0 218,385 + -0.4 218,385 + -0.9 218,386 - +1.4 218,386 - -0.7 218,386 - -2.2 218,596 + +1.6 218,610 + +0.4 218,614 + -0.1 218,615 - -0.4 218,820 + Rv0187 0.17 -0.8 218,981 + Rv0187 0.42 -1.9 218,981 + Rv0187 0.42 +3.3 218,982 - Rv0187 0.42 +0.2 219,148 + Rv0187 0.67 +1.8 219,205 + Rv0187 0.75 +1.8 219,206 - Rv0187 0.76 -0.2 219,298 - Rv0187 0.89 +0.0
Or see this region's nucleotide sequence