Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0186

Experiment: 7H9 with Phenazine ethosulfate 0.0137 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0184 and Rv0185 overlap by 4 nucleotidesRv0185 and Rv0186 are separated by 44 nucleotidesRv0186 and Rv0186A are separated by 45 nucleotidesRv0186A and Rv0187 are separated by 153 nucleotides Rv0184: Rv0184 - Conserved hypothetical protein, at 214,969 to 215,718 Rv0184 Rv0185: Rv0185 - Conserved hypothetical protein, at 215,715 to 216,224 Rv0185 Rv0186: Rv0186 - Probable beta-glucosidase BglS (gentiobiase) (cellobiase) (beta-D-glucoside glucohydrolase), at 216,269 to 218,344 Rv0186 Rv0186A: Rv0186A - Metallothionein, MymT, at 218,390 to 218,551 Rv0186A Rv0187: Rv0187 - Probable O-methyltransferase, at 218,705 to 219,367 Rv0187 Position (kb) 216 217 218 219Strain fitness (log2 ratio) -2 -1 0 1 2 3at 215.318 kb on + strand, within Rv0184at 215.319 kb on - strand, within Rv0184at 215.319 kb on - strand, within Rv0184at 215.320 kb on + strand, within Rv0184at 215.761 kb on - strandat 215.895 kb on + strand, within Rv0185at 215.895 kb on + strand, within Rv0185at 215.896 kb on - strand, within Rv0185at 216.020 kb on + strand, within Rv0185at 216.021 kb on - strand, within Rv0185at 216.076 kb on + strand, within Rv0185at 216.076 kb on + strand, within Rv0185at 216.202 kb on - strandat 216.378 kb on - strandat 216.378 kb on - strandat 216.869 kb on + strand, within Rv0186at 217.101 kb on + strand, within Rv0186at 217.298 kb on - strand, within Rv0186at 217.343 kb on + strand, within Rv0186at 217.988 kb on + strand, within Rv0186at 217.989 kb on - strand, within Rv0186at 218.099 kb on + strand, within Rv0186at 218.100 kb on - strand, within Rv0186at 218.100 kb on - strand, within Rv0186at 218.100 kb on - strand, within Rv0186at 218.100 kb on - strand, within Rv0186at 218.102 kb on - strand, within Rv0186at 218.385 kb on + strandat 218.385 kb on + strandat 218.386 kb on - strandat 218.386 kb on - strandat 218.386 kb on - strandat 218.596 kb on + strandat 218.610 kb on + strandat 218.614 kb on + strandat 218.615 kb on - strandat 218.820 kb on + strand, within Rv0187at 218.981 kb on + strand, within Rv0187at 218.981 kb on + strand, within Rv0187at 218.982 kb on - strand, within Rv0187at 219.148 kb on + strand, within Rv0187at 219.205 kb on + strand, within Rv0187at 219.206 kb on - strand, within Rv0187at 219.298 kb on - strand, within Rv0187

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Phenazine ethosulfate 0.0137 mM
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215,318 + Rv0184 0.47 -0.6
215,319 - Rv0184 0.47 +2.8
215,319 - Rv0184 0.47 -1.2
215,320 + Rv0184 0.47 -0.8
215,761 - -0.1
215,895 + Rv0185 0.35 -0.6
215,895 + Rv0185 0.35 +0.2
215,896 - Rv0185 0.35 -0.4
216,020 + Rv0185 0.60 -0.1
216,021 - Rv0185 0.60 -0.7
216,076 + Rv0185 0.71 -0.4
216,076 + Rv0185 0.71 -0.0
216,202 - +0.6
216,378 - -0.2
216,378 - +0.2
216,869 + Rv0186 0.29 -1.6
217,101 + Rv0186 0.40 -0.7
217,298 - Rv0186 0.50 -2.3
217,343 + Rv0186 0.52 -1.1
217,988 + Rv0186 0.83 -1.2
217,989 - Rv0186 0.83 -0.1
218,099 + Rv0186 0.88 -0.1
218,100 - Rv0186 0.88 +0.2
218,100 - Rv0186 0.88 -0.3
218,100 - Rv0186 0.88 -0.0
218,100 - Rv0186 0.88 +0.6
218,102 - Rv0186 0.88 -2.0
218,385 + -0.4
218,385 + -0.9
218,386 - +1.4
218,386 - -0.7
218,386 - -2.2
218,596 + +1.6
218,610 + +0.4
218,614 + -0.1
218,615 - -0.4
218,820 + Rv0187 0.17 -0.8
218,981 + Rv0187 0.42 -1.9
218,981 + Rv0187 0.42 +3.3
218,982 - Rv0187 0.42 +0.2
219,148 + Rv0187 0.67 +1.8
219,205 + Rv0187 0.75 +1.8
219,206 - Rv0187 0.76 -0.2
219,298 - Rv0187 0.89 +0.0

Or see this region's nucleotide sequence