Experiment: 7H9 with Phenazine ethosulfate 0.0137 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv2237 and Rv2237A are separated by 94 nucleotides Rv2237A and GFF2321 are separated by 10 nucleotides GFF2321 and Rv2238c are separated by 45 nucleotides Rv2238c and Rv2239c overlap by 1 nucleotides Rv2239c and Rv2240c are separated by 37 nucleotides Rv2240c and Rv2241 are separated by 258 nucleotides
Rv2237: Rv2237 - Conserved protein, at 2,509,489 to 2,510,256
Rv2237
Rv2237A: Rv2237A - Conserved protein, at 2,510,351 to 2,510,587
Rv2237A
GFF2321 - tRNA-Val, at 2,510,598 to 2,510,669
GFF2321
Rv2238c: Rv2238c - Probable peroxiredoxin AhpE, at 2,510,715 to 2,511,176
Rv2238c
Rv2239c: Rv2239c - Conserved hypothetical protein, at 2,511,176 to 2,511,652
Rv2239c
Rv2240c: Rv2240c - Unknown protein, at 2,511,690 to 2,512,280
Rv2240c
Rv2241: Rv2241 - Pyruvate dehydrogenase E1 component AceE (pyruvate decarboxylase) (pyruvate dehydrogenase) (pyruvic dehydrogenase), at 2,512,539 to 2,515,244
Rv2241
Position (kb)
2511
2512 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2510.207 kb on - strand at 2510.437 kb on - strand, within Rv2237A at 2510.437 kb on - strand, within Rv2237A at 2510.592 kb on - strand at 2510.724 kb on - strand at 2511.820 kb on + strand, within Rv2240c at 2511.820 kb on + strand, within Rv2240c at 2511.821 kb on - strand, within Rv2240c at 2512.407 kb on + strand at 2512.407 kb on + strand at 2512.407 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Phenazine ethosulfate 0.0137 mM remove 2,510,207 - -1.5 2,510,437 - Rv2237A 0.36 -0.4 2,510,437 - Rv2237A 0.36 +2.0 2,510,592 - +0.0 2,510,724 - -0.6 2,511,820 + Rv2240c 0.22 +2.0 2,511,820 + Rv2240c 0.22 -3.0 2,511,821 - Rv2240c 0.22 -1.9 2,512,407 + +0.4 2,512,407 + -0.1 2,512,407 + -0.8
Or see this region's nucleotide sequence