Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2535c

Experiment: 7H9 with Dicloxacillin 0.0069 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2533c and Rv2534c are separated by 2 nucleotidesRv2534c and Rv2535c are separated by 9 nucleotidesRv2535c and Rv2536 are separated by 33 nucleotidesRv2536 and Rv2537c are separated by 3 nucleotides Rv2533c: Rv2533c - N utilization substance protein NusB (NusB protein), at 2,858,254 to 2,858,724 Rv2533c Rv2534c: Rv2534c - Probable elongation factor P Efp, at 2,858,727 to 2,859,290 Rv2534c Rv2535c: Rv2535c - Probable cytoplasmic peptidase PepQ, at 2,859,300 to 2,860,418 Rv2535c Rv2536: Rv2536 - Probable conserved transmembrane protein, at 2,860,452 to 2,861,144 Rv2536 Rv2537c: Rv2537c - 3-dehydroquinate dehydratase AroD (AROQ) (3-dehydroquinase) (type II dhqase), at 2,861,148 to 2,861,591 Rv2537c Position (kb) 2859 2860 2861Strain fitness (log2 ratio) -2 -1 0 1 2at 2860.324 kb on + strandat 2860.432 kb on - strandat 2860.457 kb on + strandat 2860.458 kb on - strandat 2860.570 kb on + strand, within Rv2536at 2860.571 kb on - strand, within Rv2536at 2860.571 kb on - strand, within Rv2536at 2860.571 kb on - strand, within Rv2536at 2860.571 kb on - strand, within Rv2536at 2860.755 kb on + strand, within Rv2536at 2860.755 kb on + strand, within Rv2536at 2860.936 kb on + strand, within Rv2536at 2860.937 kb on - strand, within Rv2536at 2861.069 kb on + strand, within Rv2536at 2861.085 kb on - strandat 2861.142 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Dicloxacillin 0.0069 mM
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2,860,324 + +0.3
2,860,432 - +0.3
2,860,457 + -1.3
2,860,458 - -1.2
2,860,570 + Rv2536 0.17 -1.1
2,860,571 - Rv2536 0.17 -0.8
2,860,571 - Rv2536 0.17 -1.8
2,860,571 - Rv2536 0.17 -1.0
2,860,571 - Rv2536 0.17 -0.1
2,860,755 + Rv2536 0.44 -0.6
2,860,755 + Rv2536 0.44 +2.0
2,860,936 + Rv2536 0.70 -0.4
2,860,937 - Rv2536 0.70 +0.2
2,861,069 + Rv2536 0.89 -0.1
2,861,085 - -0.6
2,861,142 + +0.5

Or see this region's nucleotide sequence