Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2197c

Experiment: 7H9 with Novobiocin 0.004 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2196 and Rv2197c are separated by 176 nucleotidesRv2197c and Rv2198c overlap by 1 nucleotides Rv2196: Rv2196 - Probable ubiquinol-cytochrome C reductase QcrB (cytochrome B subunit), at 2,459,678 to 2,461,327 Rv2196 Rv2197c: Rv2197c - Probable conserved transmembrane protein, at 2,461,504 to 2,462,148 Rv2197c Rv2198c: Rv2198c - Probable conserved membrane protein MmpS3, at 2,462,148 to 2,463,047 Rv2198c Position (kb) 2461 2462 2463Strain fitness (log2 ratio) -2 -1 0 1 2at 2461.326 kb on - strandat 2461.547 kb on + strandat 2461.549 kb on + strandat 2461.549 kb on + strandat 2461.550 kb on - strandat 2461.599 kb on - strand, within Rv2197cat 2461.599 kb on - strand, within Rv2197cat 2461.641 kb on - strand, within Rv2197cat 2461.641 kb on - strand, within Rv2197cat 2461.641 kb on - strand, within Rv2197cat 2461.641 kb on - strand, within Rv2197cat 2461.943 kb on - strand, within Rv2197cat 2461.949 kb on + strand, within Rv2197cat 2462.107 kb on + strandat 2462.108 kb on - strandat 2462.136 kb on - strandat 2462.728 kb on - strand, within Rv2198cat 2463.056 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Novobiocin 0.004 mM
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2,461,326 - +0.4
2,461,547 + -0.1
2,461,549 + -1.2
2,461,549 + -1.1
2,461,550 - -0.3
2,461,599 - Rv2197c 0.15 -0.7
2,461,599 - Rv2197c 0.15 -0.5
2,461,641 - Rv2197c 0.21 -1.2
2,461,641 - Rv2197c 0.21 +1.9
2,461,641 - Rv2197c 0.21 +0.0
2,461,641 - Rv2197c 0.21 -0.7
2,461,943 - Rv2197c 0.68 -1.1
2,461,949 + Rv2197c 0.69 -1.9
2,462,107 + +0.1
2,462,108 - +0.1
2,462,136 - -2.0
2,462,728 - Rv2198c 0.64 -0.6
2,463,056 - -1.3

Or see this region's nucleotide sequence