Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3686c

Experiment: 7H9 with Chloramphenicol 0.01 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3685c and Rv3686c are separated by 25 nucleotidesRv3686c and Rv3687c are separated by 239 nucleotidesRv3687c and Rv3688c are separated by 201 nucleotides Rv3685c: Rv3685c - Probable cytochrome P450 137 Cyp137, at 4,127,295 to 4,128,725 Rv3685c Rv3686c: Rv3686c - Conserved hypothetical protein, at 4,128,751 to 4,129,083 Rv3686c Rv3687c: Rv3687c - Anti-anti-sigma factor RsfB (anti-sigma factor antagonist) (regulator of sigma F B), at 4,129,323 to 4,129,691 Rv3687c Rv3688c: Rv3688c - Conserved protein, at 4,129,893 to 4,130,357 Rv3688c Position (kb) 4128 4129 4130Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4128.046 kb on + strand, within Rv3685cat 4128.218 kb on + strand, within Rv3685cat 4128.219 kb on - strand, within Rv3685cat 4128.219 kb on - strand, within Rv3685cat 4128.219 kb on - strand, within Rv3685cat 4128.219 kb on - strand, within Rv3685cat 4128.260 kb on - strand, within Rv3685cat 4128.282 kb on + strand, within Rv3685cat 4128.283 kb on - strand, within Rv3685cat 4128.283 kb on - strand, within Rv3685cat 4128.283 kb on - strand, within Rv3685cat 4128.355 kb on + strand, within Rv3685cat 4128.356 kb on - strand, within Rv3685cat 4128.553 kb on + strand, within Rv3685cat 4128.661 kb on - strandat 4128.844 kb on - strand, within Rv3686cat 4128.844 kb on - strand, within Rv3686cat 4128.844 kb on - strand, within Rv3686cat 4128.844 kb on - strand, within Rv3686cat 4128.861 kb on + strand, within Rv3686cat 4128.863 kb on + strand, within Rv3686cat 4128.863 kb on + strand, within Rv3686cat 4128.863 kb on + strand, within Rv3686cat 4128.864 kb on - strand, within Rv3686cat 4128.946 kb on + strand, within Rv3686cat 4128.947 kb on - strand, within Rv3686cat 4129.076 kb on + strandat 4129.077 kb on - strandat 4129.126 kb on + strandat 4129.127 kb on - strandat 4129.277 kb on + strandat 4129.278 kb on - strandat 4129.278 kb on - strandat 4129.278 kb on - strandat 4129.316 kb on + strandat 4129.347 kb on + strandat 4129.649 kb on + strand, within Rv3687cat 4129.846 kb on - strandat 4129.871 kb on + strandat 4129.872 kb on - strandat 4129.873 kb on + strandat 4129.873 kb on + strandat 4129.873 kb on + strandat 4129.873 kb on + strandat 4129.873 kb on + strandat 4129.874 kb on - strandat 4129.874 kb on - strandat 4129.894 kb on + strandat 4129.895 kb on - strandat 4129.895 kb on - strandat 4129.896 kb on + strandat 4130.083 kb on + strand, within Rv3688cat 4130.083 kb on + strand, within Rv3688cat 4130.083 kb on + strand, within Rv3688c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Chloramphenicol 0.01 mM
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4,128,046 + Rv3685c 0.52 -0.1
4,128,218 + Rv3685c 0.65 -0.0
4,128,219 - Rv3685c 0.65 -0.4
4,128,219 - Rv3685c 0.65 -0.6
4,128,219 - Rv3685c 0.65 -0.4
4,128,219 - Rv3685c 0.65 -1.0
4,128,260 - Rv3685c 0.67 +0.4
4,128,282 + Rv3685c 0.69 -0.5
4,128,283 - Rv3685c 0.69 -0.6
4,128,283 - Rv3685c 0.69 +0.8
4,128,283 - Rv3685c 0.69 -0.6
4,128,355 + Rv3685c 0.74 -0.4
4,128,356 - Rv3685c 0.74 +0.6
4,128,553 + Rv3685c 0.88 -1.6
4,128,661 - -0.1
4,128,844 - Rv3686c 0.28 +0.1
4,128,844 - Rv3686c 0.28 -0.4
4,128,844 - Rv3686c 0.28 +0.6
4,128,844 - Rv3686c 0.28 -0.3
4,128,861 + Rv3686c 0.33 +0.7
4,128,863 + Rv3686c 0.34 +0.1
4,128,863 + Rv3686c 0.34 +0.0
4,128,863 + Rv3686c 0.34 +2.8
4,128,864 - Rv3686c 0.34 +0.1
4,128,946 + Rv3686c 0.59 +0.1
4,128,947 - Rv3686c 0.59 -0.3
4,129,076 + -0.4
4,129,077 - -0.1
4,129,126 + -0.8
4,129,127 - -0.4
4,129,277 + -2.7
4,129,278 - -1.5
4,129,278 - -0.6
4,129,278 - +0.0
4,129,316 + -0.2
4,129,347 + -0.2
4,129,649 + Rv3687c 0.88 +0.8
4,129,846 - -2.3
4,129,871 + -0.8
4,129,872 - -0.4
4,129,873 + -0.6
4,129,873 + +1.0
4,129,873 + -0.1
4,129,873 + +0.2
4,129,873 + +0.8
4,129,874 - -0.8
4,129,874 - -0.1
4,129,894 + +1.2
4,129,895 - -0.2
4,129,895 - +0.3
4,129,896 + -0.4
4,130,083 + Rv3688c 0.41 -0.3
4,130,083 + Rv3688c 0.41 -0.3
4,130,083 + Rv3688c 0.41 -0.3

Or see this region's nucleotide sequence