Experiment: 7H9 with Chloramphenicol 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3222c and Rv3223c overlap by 4 nucleotides Rv3223c and Rv3224 are separated by 299 nucleotides Rv3224 and Rv3224A overlap by 65 nucleotides Rv3224A and Rv3224B overlap by 23 nucleotides Rv3224B and Rv3225c overlap by 4 nucleotides
Rv3222c: Rv3222c - Conserved hypothetical protein, at 3,598,353 to 3,598,904
Rv3222c
Rv3223c: Rv3223c - Alternative RNA polymerase sigma-E factor (sigma-24) SigH (RPOE), at 3,598,901 to 3,599,551
Rv3223c
Rv3224: Rv3224 - Possible iron-regulated short-chain dehydrogenase/reductase, at 3,599,851 to 3,600,699
Rv3224
Rv3224A: Rv3224A - Conserved hypothetical protein, at 3,600,635 to 3,600,823
Rv3224A
Rv3224B: Rv3224B - Conserved hypothetical protein, at 3,600,801 to 3,601,019
Rv3224B
Rv3225c: Rv3225c - GCN5-related N-acetyltransferase, phosphorylase, at 3,601,016 to 3,602,440
Rv3225c
Position (kb)
3599
3600
3601 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3599.082 kb on + strand, within Rv3223c at 3599.083 kb on - strand, within Rv3223c at 3599.153 kb on + strand, within Rv3223c at 3599.271 kb on + strand, within Rv3223c at 3599.283 kb on + strand, within Rv3223c at 3599.284 kb on - strand, within Rv3223c at 3599.284 kb on - strand, within Rv3223c at 3599.356 kb on - strand, within Rv3223c at 3599.356 kb on - strand, within Rv3223c at 3599.431 kb on - strand, within Rv3223c at 3599.431 kb on - strand, within Rv3223c at 3599.559 kb on - strand at 3599.559 kb on - strand at 3599.690 kb on + strand at 3599.690 kb on + strand at 3599.691 kb on - strand at 3599.691 kb on - strand at 3599.691 kb on - strand at 3599.778 kb on + strand at 3600.123 kb on + strand, within Rv3224 at 3600.124 kb on - strand, within Rv3224 at 3600.124 kb on - strand, within Rv3224 at 3600.124 kb on - strand, within Rv3224 at 3600.155 kb on + strand, within Rv3224 at 3600.155 kb on + strand, within Rv3224 at 3600.156 kb on - strand, within Rv3224 at 3600.223 kb on + strand, within Rv3224 at 3600.223 kb on + strand, within Rv3224 at 3600.224 kb on - strand, within Rv3224 at 3600.224 kb on - strand, within Rv3224 at 3600.224 kb on - strand, within Rv3224 at 3600.224 kb on - strand, within Rv3224 at 3600.230 kb on + strand, within Rv3224 at 3600.231 kb on - strand, within Rv3224 at 3600.252 kb on + strand, within Rv3224 at 3600.252 kb on + strand, within Rv3224 at 3600.253 kb on - strand, within Rv3224 at 3600.253 kb on - strand, within Rv3224 at 3600.334 kb on + strand, within Rv3224 at 3600.334 kb on + strand, within Rv3224 at 3600.335 kb on - strand, within Rv3224 at 3600.335 kb on - strand, within Rv3224 at 3600.335 kb on - strand, within Rv3224 at 3600.335 kb on - strand, within Rv3224 at 3600.349 kb on + strand, within Rv3224 at 3600.349 kb on + strand, within Rv3224 at 3600.349 kb on + strand, within Rv3224 at 3600.507 kb on - strand, within Rv3224 at 3600.507 kb on - strand, within Rv3224 at 3600.508 kb on + strand, within Rv3224 at 3600.508 kb on + strand, within Rv3224 at 3600.508 kb on + strand, within Rv3224 at 3600.509 kb on - strand, within Rv3224 at 3600.523 kb on + strand, within Rv3224 at 3600.523 kb on + strand, within Rv3224 at 3600.523 kb on + strand, within Rv3224 at 3600.524 kb on - strand, within Rv3224 at 3600.554 kb on - strand, within Rv3224 at 3600.619 kb on + strand at 3600.822 kb on - strand at 3601.017 kb on + strand at 3601.030 kb on + strand at 3601.111 kb on - strand at 3601.114 kb on - strand at 3601.379 kb on + strand, within Rv3225c at 3601.451 kb on + strand, within Rv3225c at 3601.685 kb on + strand, within Rv3225c
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Chloramphenicol 0.01 mM remove 3,599,082 + Rv3223c 0.28 +0.3 3,599,083 - Rv3223c 0.28 +2.5 3,599,153 + Rv3223c 0.39 +1.0 3,599,271 + Rv3223c 0.57 -1.1 3,599,283 + Rv3223c 0.59 +0.1 3,599,284 - Rv3223c 0.59 -1.2 3,599,284 - Rv3223c 0.59 -1.1 3,599,356 - Rv3223c 0.70 +2.5 3,599,356 - Rv3223c 0.70 -0.1 3,599,431 - Rv3223c 0.81 +1.6 3,599,431 - Rv3223c 0.81 +0.7 3,599,559 - +0.7 3,599,559 - +0.6 3,599,690 + +0.5 3,599,690 + +0.7 3,599,691 - -1.4 3,599,691 - -1.6 3,599,691 - +0.3 3,599,778 + -0.0 3,600,123 + Rv3224 0.32 -0.5 3,600,124 - Rv3224 0.32 -0.3 3,600,124 - Rv3224 0.32 -0.6 3,600,124 - Rv3224 0.32 -1.2 3,600,155 + Rv3224 0.36 -1.5 3,600,155 + Rv3224 0.36 -0.6 3,600,156 - Rv3224 0.36 -0.1 3,600,223 + Rv3224 0.44 -0.6 3,600,223 + Rv3224 0.44 +0.4 3,600,224 - Rv3224 0.44 -0.2 3,600,224 - Rv3224 0.44 +0.6 3,600,224 - Rv3224 0.44 +0.3 3,600,224 - Rv3224 0.44 -0.5 3,600,230 + Rv3224 0.45 -1.3 3,600,231 - Rv3224 0.45 -0.0 3,600,252 + Rv3224 0.47 -1.4 3,600,252 + Rv3224 0.47 -2.4 3,600,253 - Rv3224 0.47 +0.5 3,600,253 - Rv3224 0.47 -0.3 3,600,334 + Rv3224 0.57 -0.4 3,600,334 + Rv3224 0.57 -0.5 3,600,335 - Rv3224 0.57 -0.9 3,600,335 - Rv3224 0.57 -1.0 3,600,335 - Rv3224 0.57 +0.6 3,600,335 - Rv3224 0.57 +0.1 3,600,349 + Rv3224 0.59 -0.6 3,600,349 + Rv3224 0.59 -0.9 3,600,349 + Rv3224 0.59 -0.7 3,600,507 - Rv3224 0.77 -0.0 3,600,507 - Rv3224 0.77 -0.4 3,600,508 + Rv3224 0.77 -0.4 3,600,508 + Rv3224 0.77 -1.0 3,600,508 + Rv3224 0.77 -0.8 3,600,509 - Rv3224 0.78 -1.8 3,600,523 + Rv3224 0.79 -1.0 3,600,523 + Rv3224 0.79 -0.1 3,600,523 + Rv3224 0.79 +1.1 3,600,524 - Rv3224 0.79 -0.6 3,600,554 - Rv3224 0.83 +0.6 3,600,619 + -0.4 3,600,822 - +0.9 3,601,017 + +1.1 3,601,030 + +0.1 3,601,111 - +0.1 3,601,114 - +0.2 3,601,379 + Rv3225c 0.25 -0.8 3,601,451 + Rv3225c 0.31 -0.1 3,601,685 + Rv3225c 0.47 +0.0
Or see this region's nucleotide sequence