Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3088

Experiment: 7H9 with Chloramphenicol 0.01 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3087 and Rv3088 overlap by 4 nucleotidesRv3088 and Rv3089 overlap by 4 nucleotides Rv3087: Rv3087 - Possible triacylglycerol synthase (diacylglycerol acyltransferase), at 3,452,925 to 3,454,343 Rv3087 Rv3088: Rv3088 - Putative triacylglycerol synthase (diacylglycerol acyltransferase) Tgs4, at 3,454,340 to 3,455,764 Rv3088 Rv3089: Rv3089 - Probable chain-fatty-acid-CoA ligase FadD13 (fatty-acyl-CoA synthetase), at 3,455,761 to 3,457,272 Rv3089 Position (kb) 3454 3455 3456Strain fitness (log2 ratio) -1 0 1at 3453.387 kb on + strand, within Rv3087at 3453.388 kb on - strand, within Rv3087at 3453.388 kb on - strand, within Rv3087at 3453.388 kb on - strand, within Rv3087at 3453.760 kb on - strand, within Rv3087at 3453.760 kb on - strand, within Rv3087at 3453.856 kb on - strand, within Rv3087at 3453.870 kb on + strand, within Rv3087at 3454.223 kb on - strandat 3454.479 kb on - strandat 3454.688 kb on + strand, within Rv3088at 3454.688 kb on + strand, within Rv3088at 3454.688 kb on + strand, within Rv3088at 3454.688 kb on + strand, within Rv3088at 3454.689 kb on - strand, within Rv3088at 3455.528 kb on + strand, within Rv3088at 3455.975 kb on - strand, within Rv3089at 3456.009 kb on + strand, within Rv3089at 3456.009 kb on + strand, within Rv3089at 3456.248 kb on - strand, within Rv3089at 3456.628 kb on + strand, within Rv3089

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Chloramphenicol 0.01 mM
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3,453,387 + Rv3087 0.33 +0.2
3,453,388 - Rv3087 0.33 -0.4
3,453,388 - Rv3087 0.33 -0.4
3,453,388 - Rv3087 0.33 +0.5
3,453,760 - Rv3087 0.59 +0.6
3,453,760 - Rv3087 0.59 -0.0
3,453,856 - Rv3087 0.66 -0.2
3,453,870 + Rv3087 0.67 +0.0
3,454,223 - +0.1
3,454,479 - -0.5
3,454,688 + Rv3088 0.24 +0.9
3,454,688 + Rv3088 0.24 +0.2
3,454,688 + Rv3088 0.24 -0.1
3,454,688 + Rv3088 0.24 +0.4
3,454,689 - Rv3088 0.24 +0.2
3,455,528 + Rv3088 0.83 +0.1
3,455,975 - Rv3089 0.14 +0.9
3,456,009 + Rv3089 0.16 +0.4
3,456,009 + Rv3089 0.16 +0.4
3,456,248 - Rv3089 0.32 -0.1
3,456,628 + Rv3089 0.57 -0.7

Or see this region's nucleotide sequence