Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2755c

Experiment: 7H9 with Chloramphenicol 0.01 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2754c and Rv2755c are separated by 243 nucleotidesRv2755c and Rv2756c overlap by 4 nucleotides Rv2754c: Rv2754c - Probable thymidylate synthase ThyX (ts) (TSase), at 3,067,193 to 3,067,945 Rv2754c Rv2755c: Rv2755c - Possible type I restriction/modification system specificity determinant (fragment) HsdS.1 (S protein), at 3,068,189 to 3,068,464 Rv2755c Rv2756c: Rv2756c - Possible type I restriction/modification system DNA methylase HsdM (M protein) (DNA methyltransferase), at 3,068,461 to 3,070,083 Rv2756c Position (kb) 3068 3069Strain fitness (log2 ratio) -1 0 1at 3067.973 kb on + strandat 3068.081 kb on + strandat 3068.226 kb on - strand, within Rv2755cat 3068.320 kb on - strand, within Rv2755cat 3068.320 kb on - strand, within Rv2755cat 3068.338 kb on + strand, within Rv2755cat 3068.369 kb on + strand, within Rv2755cat 3068.467 kb on - strandat 3068.689 kb on - strand, within Rv2756cat 3069.059 kb on + strand, within Rv2756cat 3069.321 kb on - strand, within Rv2756c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Chloramphenicol 0.01 mM
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3,067,973 + +0.3
3,068,081 + +0.4
3,068,226 - Rv2755c 0.13 -0.3
3,068,320 - Rv2755c 0.47 +1.1
3,068,320 - Rv2755c 0.47 +0.4
3,068,338 + Rv2755c 0.54 +0.4
3,068,369 + Rv2755c 0.65 -0.1
3,068,467 - +0.6
3,068,689 - Rv2756c 0.14 +0.1
3,069,059 + Rv2756c 0.37 -0.4
3,069,321 - Rv2756c 0.53 -0.5

Or see this region's nucleotide sequence