Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2750

Experiment: 7H9 with Chloramphenicol 0.01 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2748c and Rv2749 overlap by 2 nucleotidesRv2749 and Rv2750 overlap by 4 nucleotidesRv2750 and Rv2751 are separated by 3 nucleotidesRv2751 and Rv2752c overlap by 14 nucleotides Rv2748c: Rv2748c - Possible cell division transmembrane protein FtsK, at 3,059,855 to 3,062,506 Rv2748c Rv2749: Rv2749 - Conserved protein, at 3,062,505 to 3,062,819 Rv2749 Rv2750: Rv2750 - Probable dehydrogenase, at 3,062,816 to 3,063,634 Rv2750 Rv2751: Rv2751 - Conserved protein, at 3,063,638 to 3,064,528 Rv2751 Rv2752c: Rv2752c - Conserved hypothetical protein, at 3,064,515 to 3,066,191 Rv2752c Position (kb) 3062 3063 3064Strain fitness (log2 ratio) -1 0 1at 3062.370 kb on - strandat 3062.381 kb on + strandat 3062.382 kb on - strandat 3062.620 kb on - strand, within Rv2749at 3062.693 kb on + strand, within Rv2749at 3063.211 kb on - strand, within Rv2750at 3063.211 kb on - strand, within Rv2750at 3063.427 kb on + strand, within Rv2750at 3063.632 kb on + strandat 3063.810 kb on + strand, within Rv2751at 3063.811 kb on - strand, within Rv2751at 3064.248 kb on + strand, within Rv2751at 3064.249 kb on - strand, within Rv2751at 3064.257 kb on - strand, within Rv2751at 3064.311 kb on - strand, within Rv2751at 3064.369 kb on - strand, within Rv2751at 3064.517 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Chloramphenicol 0.01 mM
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3,062,370 - +1.1
3,062,381 + -1.3
3,062,382 - -0.6
3,062,620 - Rv2749 0.37 -0.7
3,062,693 + Rv2749 0.60 -0.2
3,063,211 - Rv2750 0.48 +1.7
3,063,211 - Rv2750 0.48 +0.6
3,063,427 + Rv2750 0.75 -0.0
3,063,632 + +1.5
3,063,810 + Rv2751 0.19 +0.4
3,063,811 - Rv2751 0.19 -0.6
3,064,248 + Rv2751 0.68 +0.0
3,064,249 - Rv2751 0.69 -0.1
3,064,257 - Rv2751 0.69 -0.7
3,064,311 - Rv2751 0.76 +0.2
3,064,369 - Rv2751 0.82 +0.5
3,064,517 - +0.7

Or see this region's nucleotide sequence