Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2613c

Experiment: 7H9 with Chloramphenicol 0.01 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2611c and Rv2612c overlap by 4 nucleotidesRv2612c and Rv2613c overlap by 4 nucleotidesRv2613c and Rv2614c overlap by 8 nucleotides Rv2611c: Rv2611c - Probable acyltransferase, at 2,939,012 to 2,939,962 Rv2611c Rv2612c: Rv2612c - PI synthase PgsA1 (phosphatidylinositol synthase) (CDP-diacylglycerol--inositol-3-phosphatidyltransferase), at 2,939,959 to 2,940,612 Rv2612c Rv2613c: Rv2613c - Conserved protein, at 2,940,609 to 2,941,196 Rv2613c Rv2614c: Rv2614c - Probable threonyl-tRNA synthetase ThrS (threonine-tRNA synthetase)(ThrRS) (threonine-tRNA ligase), at 2,941,189 to 2,943,267 Rv2614c Position (kb) 2940 2941 2942Strain fitness (log2 ratio) -1 0 1at 2939.948 kb on - strandat 2940.778 kb on - strand, within Rv2613cat 2940.778 kb on - strand, within Rv2613cat 2940.938 kb on - strand, within Rv2613cat 2941.085 kb on - strand, within Rv2613cat 2941.085 kb on - strand, within Rv2613cat 2941.225 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Chloramphenicol 0.01 mM
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2,939,948 - -0.8
2,940,778 - Rv2613c 0.29 +0.3
2,940,778 - Rv2613c 0.29 -0.7
2,940,938 - Rv2613c 0.56 -1.2
2,941,085 - Rv2613c 0.81 -1.4
2,941,085 - Rv2613c 0.81 -0.4
2,941,225 - -1.3

Or see this region's nucleotide sequence