Experiment: 7H9 with Chloramphenicol 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv1713 and Rv1714 are separated by 173 nucleotides Rv1714 and Rv1715 overlap by 7 nucleotides Rv1715 and Rv1716 are separated by 2 nucleotides Rv1716 and Rv1717 overlap by 1 nucleotides
Rv1713: Rv1713 - Probable GTP-binding protein EngA, at 1,940,288 to 1,941,679
Rv1713
Rv1714: Rv1714 - Probable oxidoreductase, at 1,941,853 to 1,942,665
Rv1714
Rv1715: Rv1715 - Probable 3-hydroxybutyryl-CoA dehydrogenase FadB3 (beta-hydroxybutyryl-CoA dehydrogenase) (BHBD), at 1,942,659 to 1,943,573
Rv1715
Rv1716: Rv1716 - Conserved hypothetical protein, at 1,943,576 to 1,944,406
Rv1716
Rv1717: Rv1717 - Conserved hypothetical protein, at 1,944,406 to 1,944,756
Rv1717
Position (kb)
1942
1943
1944 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1942.191 kb on + strand, within Rv1714 at 1942.192 kb on - strand, within Rv1714 at 1942.636 kb on + strand at 1942.859 kb on - strand, within Rv1715 at 1943.340 kb on + strand, within Rv1715 at 1943.340 kb on + strand, within Rv1715 at 1943.625 kb on - strand at 1943.670 kb on - strand, within Rv1716 at 1943.963 kb on + strand, within Rv1716 at 1943.964 kb on - strand, within Rv1716 at 1943.964 kb on - strand, within Rv1716 at 1943.981 kb on + strand, within Rv1716 at 1943.985 kb on - strand, within Rv1716 at 1943.990 kb on + strand, within Rv1716 at 1944.239 kb on + strand, within Rv1716 at 1944.239 kb on + strand, within Rv1716 at 1944.240 kb on - strand, within Rv1716 at 1944.440 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Chloramphenicol 0.01 mM remove 1,942,191 + Rv1714 0.42 -0.1 1,942,192 - Rv1714 0.42 -0.4 1,942,636 + +0.3 1,942,859 - Rv1715 0.22 +3.9 1,943,340 + Rv1715 0.74 +0.5 1,943,340 + Rv1715 0.74 -0.3 1,943,625 - -0.3 1,943,670 - Rv1716 0.11 -0.9 1,943,963 + Rv1716 0.47 +1.7 1,943,964 - Rv1716 0.47 -0.0 1,943,964 - Rv1716 0.47 +0.8 1,943,981 + Rv1716 0.49 +0.4 1,943,985 - Rv1716 0.49 +0.6 1,943,990 + Rv1716 0.50 +0.2 1,944,239 + Rv1716 0.80 -0.7 1,944,239 + Rv1716 0.80 -0.5 1,944,240 - Rv1716 0.80 +0.4 1,944,440 - -0.4
Or see this region's nucleotide sequence