Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv1515c

Experiment: 7H9 with Chloramphenicol 0.01 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv1513 and Rv1514c are separated by 17 nucleotidesRv1514c and Rv1515c are separated by 34 nucleotidesRv1515c and Rv1516c are separated by 2 nucleotides Rv1513: Rv1513 - Conserved protein, at 1,705,058 to 1,705,789 Rv1513 Rv1514c: Rv1514c - Conserved hypothetical protein, at 1,705,807 to 1,706,595 Rv1514c Rv1515c: Rv1515c - Conserved hypothetical protein, at 1,706,630 to 1,707,526 Rv1515c Rv1516c: Rv1516c - Probable sugar transferase, at 1,707,529 to 1,708,539 Rv1516c Position (kb) 1706 1707 1708Strain fitness (log2 ratio) -1 0 1at 1705.854 kb on - strandat 1705.859 kb on + strandat 1705.876 kb on + strandat 1706.054 kb on - strand, within Rv1514cat 1706.442 kb on - strand, within Rv1514cat 1706.442 kb on - strand, within Rv1514cat 1706.476 kb on + strand, within Rv1514cat 1706.607 kb on - strandat 1706.677 kb on - strandat 1706.772 kb on - strand, within Rv1515cat 1707.070 kb on - strand, within Rv1515cat 1707.314 kb on + strand, within Rv1515cat 1707.386 kb on + strand, within Rv1515cat 1708.217 kb on - strand, within Rv1516cat 1708.217 kb on - strand, within Rv1516cat 1708.238 kb on + strand, within Rv1516c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Chloramphenicol 0.01 mM
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1,705,854 - +0.0
1,705,859 + -0.5
1,705,876 + +0.7
1,706,054 - Rv1514c 0.31 -0.7
1,706,442 - Rv1514c 0.80 -0.1
1,706,442 - Rv1514c 0.80 +0.2
1,706,476 + Rv1514c 0.85 -0.2
1,706,607 - +0.2
1,706,677 - +0.3
1,706,772 - Rv1515c 0.16 +0.5
1,707,070 - Rv1515c 0.49 +0.5
1,707,314 + Rv1515c 0.76 +0.4
1,707,386 + Rv1515c 0.84 +0.2
1,708,217 - Rv1516c 0.68 -0.8
1,708,217 - Rv1516c 0.68 +1.7
1,708,238 + Rv1516c 0.70 -0.7

Or see this region's nucleotide sequence