Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0142

Experiment: 7H9 with Chloramphenicol 0.01 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0139 and Rv0140 are separated by 60 nucleotidesRv0140 and Rv0141c overlap by 20 nucleotidesRv0141c and Rv0142 are separated by 29 nucleotidesRv0142 and Rv0143c are separated by 66 nucleotides Rv0139: Rv0139 - Possible oxidoreductase, at 165,827 to 166,849 Rv0139 Rv0140: Rv0140 - Conserved protein, at 166,910 to 167,290 Rv0140 Rv0141c: Rv0141c - Unknown protein, at 167,271 to 167,681 Rv0141c Rv0142: Rv0142 - Conserved hypothetical protein, at 167,711 to 168,637 Rv0142 Rv0143c: Rv0143c - Probable conserved transmembrane protein, at 168,704 to 170,182 Rv0143c Position (kb) 167 168 169Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 166.863 kb on - strandat 166.986 kb on + strand, within Rv0140at 167.065 kb on + strand, within Rv0140at 167.085 kb on + strand, within Rv0140at 167.120 kb on + strand, within Rv0140at 167.121 kb on - strand, within Rv0140at 167.148 kb on - strand, within Rv0140at 167.148 kb on - strand, within Rv0140at 167.150 kb on + strand, within Rv0140at 167.201 kb on + strand, within Rv0140at 167.202 kb on - strand, within Rv0140at 167.308 kb on - strandat 167.308 kb on - strandat 167.335 kb on + strand, within Rv0141cat 167.398 kb on + strand, within Rv0141cat 167.570 kb on - strand, within Rv0141cat 167.839 kb on + strand, within Rv0142at 167.839 kb on + strand, within Rv0142at 167.840 kb on - strand, within Rv0142at 167.840 kb on - strand, within Rv0142at 167.840 kb on - strand, within Rv0142at 168.158 kb on + strand, within Rv0142at 168.158 kb on + strand, within Rv0142at 168.158 kb on + strand, within Rv0142at 168.384 kb on + strand, within Rv0142at 168.384 kb on + strand, within Rv0142at 168.449 kb on + strand, within Rv0142at 168.635 kb on + strandat 168.636 kb on - strandat 168.777 kb on + strandat 168.777 kb on + strandat 168.777 kb on + strandat 169.043 kb on + strand, within Rv0143cat 169.044 kb on - strand, within Rv0143cat 169.208 kb on + strand, within Rv0143cat 169.208 kb on + strand, within Rv0143cat 169.209 kb on - strand, within Rv0143cat 169.209 kb on - strand, within Rv0143cat 169.273 kb on + strand, within Rv0143cat 169.305 kb on + strand, within Rv0143cat 169.305 kb on + strand, within Rv0143cat 169.305 kb on + strand, within Rv0143cat 169.305 kb on + strand, within Rv0143cat 169.306 kb on - strand, within Rv0143cat 169.306 kb on - strand, within Rv0143cat 169.311 kb on + strand, within Rv0143cat 169.311 kb on + strand, within Rv0143cat 169.311 kb on + strand, within Rv0143cat 169.312 kb on - strand, within Rv0143cat 169.355 kb on + strand, within Rv0143cat 169.355 kb on + strand, within Rv0143cat 169.380 kb on + strand, within Rv0143cat 169.380 kb on + strand, within Rv0143cat 169.395 kb on + strand, within Rv0143cat 169.483 kb on - strand, within Rv0143cat 169.568 kb on + strand, within Rv0143c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Chloramphenicol 0.01 mM
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166,863 - -0.3
166,986 + Rv0140 0.20 +0.4
167,065 + Rv0140 0.41 -0.1
167,085 + Rv0140 0.46 -0.9
167,120 + Rv0140 0.55 +0.3
167,121 - Rv0140 0.55 +0.5
167,148 - Rv0140 0.62 -0.3
167,148 - Rv0140 0.62 -0.8
167,150 + Rv0140 0.63 +1.8
167,201 + Rv0140 0.76 -0.9
167,202 - Rv0140 0.77 +0.4
167,308 - +0.2
167,308 - -0.6
167,335 + Rv0141c 0.16 -0.2
167,398 + Rv0141c 0.31 -0.1
167,570 - Rv0141c 0.73 -2.0
167,839 + Rv0142 0.14 -0.6
167,839 + Rv0142 0.14 +0.2
167,840 - Rv0142 0.14 +0.1
167,840 - Rv0142 0.14 -0.2
167,840 - Rv0142 0.14 +1.8
168,158 + Rv0142 0.48 -0.1
168,158 + Rv0142 0.48 -0.1
168,158 + Rv0142 0.48 +1.2
168,384 + Rv0142 0.73 -0.1
168,384 + Rv0142 0.73 -0.1
168,449 + Rv0142 0.80 +0.6
168,635 + -0.6
168,636 - -1.1
168,777 + +0.4
168,777 + -0.7
168,777 + +0.9
169,043 + Rv0143c 0.23 +1.8
169,044 - Rv0143c 0.23 -0.3
169,208 + Rv0143c 0.34 +0.2
169,208 + Rv0143c 0.34 -0.4
169,209 - Rv0143c 0.34 -1.0
169,209 - Rv0143c 0.34 +0.3
169,273 + Rv0143c 0.38 -0.3
169,305 + Rv0143c 0.41 -0.0
169,305 + Rv0143c 0.41 -0.4
169,305 + Rv0143c 0.41 -0.4
169,305 + Rv0143c 0.41 -0.6
169,306 - Rv0143c 0.41 -1.7
169,306 - Rv0143c 0.41 +0.3
169,311 + Rv0143c 0.41 -0.1
169,311 + Rv0143c 0.41 -0.1
169,311 + Rv0143c 0.41 -1.1
169,312 - Rv0143c 0.41 -0.2
169,355 + Rv0143c 0.44 +0.3
169,355 + Rv0143c 0.44 -2.8
169,380 + Rv0143c 0.46 +1.0
169,380 + Rv0143c 0.46 -1.9
169,395 + Rv0143c 0.47 -0.4
169,483 - Rv0143c 0.53 +1.1
169,568 + Rv0143c 0.58 +0.3

Or see this region's nucleotide sequence