Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0086

Experiment: 7H9 with Chloramphenicol 0.01 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0084 and Rv0085 are separated by 10 nucleotidesRv0085 and Rv0086 overlap by 1 nucleotidesRv0086 and Rv0087 overlap by 4 nucleotides Rv0084: Rv0084 - Possible formate hydrogenlyase HycD (FHL), at 92,328 to 93,278 Rv0084 Rv0085: Rv0085 - Possible hydrogenase HycP, at 93,289 to 93,951 Rv0085 Rv0086: Rv0086 - Possible hydrogenase HycQ, at 93,951 to 95,417 Rv0086 Rv0087: Rv0087 - Possible formate hydrogenase HycE (FHL), at 95,414 to 96,892 Rv0087 Position (kb) 93 94 95 96Strain fitness (log2 ratio) -2 -1 0 1 2 3at 92.973 kb on + strand, within Rv0084at 92.973 kb on + strand, within Rv0084at 93.774 kb on - strand, within Rv0085at 93.774 kb on - strand, within Rv0085at 94.245 kb on - strand, within Rv0086at 94.257 kb on - strand, within Rv0086at 94.428 kb on + strand, within Rv0086at 94.777 kb on - strand, within Rv0086at 95.448 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Chloramphenicol 0.01 mM
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92,973 + Rv0084 0.68 -1.8
92,973 + Rv0084 0.68 +0.8
93,774 - Rv0085 0.73 -0.2
93,774 - Rv0085 0.73 +3.4
94,245 - Rv0086 0.20 +0.8
94,257 - Rv0086 0.21 +0.2
94,428 + Rv0086 0.33 +0.2
94,777 - Rv0086 0.56 +0.3
95,448 - -0.1

Or see this region's nucleotide sequence