Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3130c

Experiment: 7H9 with 2-Mercaptopyridine N-oxide 0.0078 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3128c and Rv3129 are separated by 478 nucleotidesRv3129 and Rv3130c overlap by 18 nucleotidesRv3130c and Rv3131 are separated by 184 nucleotides Rv3128c: Rv3128c - Conserved hypothetical protein, at 3,493,168 to 3,494,181 Rv3128c Rv3129: Rv3129 - Conserved hypothetical protein, at 3,494,660 to 3,494,992 Rv3129 Rv3130c: Rv3130c - Triacylglycerol synthase (diacylglycerol acyltransferase) Tgs1, at 3,494,975 to 3,496,366 Rv3130c Rv3131: Rv3131 - Conserved protein, at 3,496,551 to 3,497,549 Rv3131 Position (kb) 3494 3495 3496 3497Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3494.213 kb on + strandat 3494.214 kb on - strandat 3494.382 kb on - strandat 3494.476 kb on + strandat 3494.476 kb on + strandat 3494.477 kb on - strandat 3495.121 kb on - strand, within Rv3130cat 3495.136 kb on - strand, within Rv3130cat 3495.192 kb on - strand, within Rv3130cat 3495.214 kb on + strand, within Rv3130cat 3495.214 kb on + strand, within Rv3130cat 3495.899 kb on - strand, within Rv3130cat 3496.191 kb on - strand, within Rv3130cat 3496.220 kb on + strand, within Rv3130cat 3496.220 kb on + strand, within Rv3130cat 3496.355 kb on + strandat 3496.356 kb on - strandat 3496.513 kb on - strandat 3496.679 kb on + strand, within Rv3131

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with 2-Mercaptopyridine N-oxide 0.0078 mM
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3,494,213 + +1.4
3,494,214 - +0.1
3,494,382 - -3.1
3,494,476 + -0.8
3,494,476 + +2.3
3,494,477 - -1.4
3,495,121 - Rv3130c 0.10 +0.8
3,495,136 - Rv3130c 0.12 -0.8
3,495,192 - Rv3130c 0.16 -0.3
3,495,214 + Rv3130c 0.17 +1.1
3,495,214 + Rv3130c 0.17 -0.5
3,495,899 - Rv3130c 0.66 -0.4
3,496,191 - Rv3130c 0.87 -0.4
3,496,220 + Rv3130c 0.89 +1.6
3,496,220 + Rv3130c 0.89 -0.2
3,496,355 + +0.3
3,496,356 - +0.8
3,496,513 - +0.4
3,496,679 + Rv3131 0.13 -0.6

Or see this region's nucleotide sequence