Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2856

Experiment: 7H9 with 2-Mercaptopyridine N-oxide 0.0078 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2855 and Rv2856 are separated by 99 nucleotidesRv2856 and Rv2857c are separated by 780 nucleotides Rv2855: Rv2855 - NADPH-dependent mycothiol reductase Mtr, at 3,165,205 to 3,166,584 Rv2855 Rv2856: Rv2856 - Possible nickel-transport integral membrane protein NicT, at 3,166,684 to 3,167,802 Rv2856 Rv2857c: Rv2857c - Probable short-chain type dehydrogenase/reductase, at 3,168,583 to 3,169,359 Rv2857c Position (kb) 3166 3167 3168Strain fitness (log2 ratio) -2 -1 0 1 2at 3166.234 kb on + strand, within Rv2855at 3166.641 kb on - strandat 3167.156 kb on - strand, within Rv2856at 3167.230 kb on + strand, within Rv2856at 3167.325 kb on + strand, within Rv2856at 3167.325 kb on + strand, within Rv2856at 3167.325 kb on + strand, within Rv2856at 3167.329 kb on + strand, within Rv2856at 3167.329 kb on + strand, within Rv2856at 3167.428 kb on + strand, within Rv2856at 3167.548 kb on + strand, within Rv2856at 3167.548 kb on + strand, within Rv2856at 3168.150 kb on - strandat 3168.282 kb on + strandat 3168.338 kb on + strandat 3168.339 kb on - strandat 3168.600 kb on - strandat 3168.668 kb on - strand, within Rv2857c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with 2-Mercaptopyridine N-oxide 0.0078 mM
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3,166,234 + Rv2855 0.75 -1.9
3,166,641 - +0.3
3,167,156 - Rv2856 0.42 -1.3
3,167,230 + Rv2856 0.49 -0.1
3,167,325 + Rv2856 0.57 +0.1
3,167,325 + Rv2856 0.57 +0.8
3,167,325 + Rv2856 0.57 -0.1
3,167,329 + Rv2856 0.58 -0.3
3,167,329 + Rv2856 0.58 +0.6
3,167,428 + Rv2856 0.66 -0.5
3,167,548 + Rv2856 0.77 -0.1
3,167,548 + Rv2856 0.77 +1.2
3,168,150 - -0.8
3,168,282 + +0.8
3,168,338 + +0.4
3,168,339 - +1.8
3,168,600 - -0.7
3,168,668 - Rv2857c 0.11 -0.1

Or see this region's nucleotide sequence