Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0271c

Experiment: 7H9 with 2-Mercaptopyridine N-oxide 0.0078 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0270 and Rv0271c are separated by 16 nucleotidesRv0271c and Rv0272c are separated by 113 nucleotides Rv0270: Rv0270 - Probable fatty-acid-CoA ligase FadD2 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase), at 324,567 to 326,249 Rv0270 Rv0271c: Rv0271c - Probable acyl-CoA dehydrogenase FadE6, at 326,266 to 328,461 Rv0271c Rv0272c: Rv0272c - Unknown protein, at 328,575 to 329,708 Rv0272c Position (kb) 326 327 328 329Strain fitness (log2 ratio) -2 -1 0 1at 325.507 kb on + strand, within Rv0270at 325.508 kb on - strand, within Rv0270at 325.508 kb on - strand, within Rv0270at 325.508 kb on - strand, within Rv0270at 325.656 kb on + strand, within Rv0270at 325.666 kb on - strand, within Rv0270at 325.909 kb on - strand, within Rv0270at 325.932 kb on + strand, within Rv0270at 325.933 kb on - strand, within Rv0270at 325.933 kb on - strand, within Rv0270at 326.058 kb on - strand, within Rv0270at 326.136 kb on + strandat 326.137 kb on - strandat 326.140 kb on + strandat 326.141 kb on - strandat 326.160 kb on + strandat 326.160 kb on + strandat 326.198 kb on - strandat 326.237 kb on - strandat 326.247 kb on + strandat 326.247 kb on + strandat 326.247 kb on + strandat 326.267 kb on + strandat 326.282 kb on - strandat 326.282 kb on - strandat 326.336 kb on + strandat 326.336 kb on + strandat 326.367 kb on - strandat 326.367 kb on - strandat 326.447 kb on + strandat 326.625 kb on - strand, within Rv0271cat 326.786 kb on + strand, within Rv0271cat 326.787 kb on - strand, within Rv0271cat 326.937 kb on - strand, within Rv0271cat 327.429 kb on - strand, within Rv0271cat 327.755 kb on + strand, within Rv0271cat 327.755 kb on + strand, within Rv0271cat 327.755 kb on + strand, within Rv0271cat 327.756 kb on - strand, within Rv0271cat 327.964 kb on + strand, within Rv0271cat 327.965 kb on - strand, within Rv0271cat 327.965 kb on - strand, within Rv0271cat 328.000 kb on + strand, within Rv0271cat 328.099 kb on + strand, within Rv0271cat 328.100 kb on - strand, within Rv0271cat 328.249 kb on + strandat 328.433 kb on + strandat 328.434 kb on - strandat 328.449 kb on + strandat 328.450 kb on - strandat 328.648 kb on + strandat 328.703 kb on - strand, within Rv0272cat 328.970 kb on + strand, within Rv0272cat 329.239 kb on + strand, within Rv0272cat 329.240 kb on - strand, within Rv0272cat 329.285 kb on + strand, within Rv0272c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with 2-Mercaptopyridine N-oxide 0.0078 mM
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325,507 + Rv0270 0.56 -0.6
325,508 - Rv0270 0.56 -0.5
325,508 - Rv0270 0.56 -0.4
325,508 - Rv0270 0.56 -1.7
325,656 + Rv0270 0.65 -0.2
325,666 - Rv0270 0.65 +0.9
325,909 - Rv0270 0.80 +0.7
325,932 + Rv0270 0.81 -2.0
325,933 - Rv0270 0.81 -1.1
325,933 - Rv0270 0.81 -0.1
326,058 - Rv0270 0.89 -0.0
326,136 + +1.7
326,137 - +0.0
326,140 + +0.0
326,141 - -2.5
326,160 + +0.2
326,160 + -1.7
326,198 - +0.4
326,237 - +0.1
326,247 + -2.7
326,247 + -0.3
326,247 + -0.5
326,267 + -1.1
326,282 - +1.5
326,282 - -1.3
326,336 + +0.2
326,336 + -1.1
326,367 - -1.1
326,367 - -0.5
326,447 + -0.5
326,625 - Rv0271c 0.16 -1.6
326,786 + Rv0271c 0.24 -0.0
326,787 - Rv0271c 0.24 -0.6
326,937 - Rv0271c 0.31 -0.9
327,429 - Rv0271c 0.53 -0.7
327,755 + Rv0271c 0.68 +0.0
327,755 + Rv0271c 0.68 -0.1
327,755 + Rv0271c 0.68 -0.7
327,756 - Rv0271c 0.68 -0.5
327,964 + Rv0271c 0.77 -0.1
327,965 - Rv0271c 0.77 -1.1
327,965 - Rv0271c 0.77 +0.0
328,000 + Rv0271c 0.79 -0.8
328,099 + Rv0271c 0.83 -0.6
328,100 - Rv0271c 0.84 +0.3
328,249 + -0.4
328,433 + +1.2
328,434 - -1.0
328,449 + -0.5
328,450 - +0.3
328,648 + -0.3
328,703 - Rv0272c 0.11 -0.1
328,970 + Rv0272c 0.35 +0.1
329,239 + Rv0272c 0.59 +0.3
329,240 - Rv0272c 0.59 -0.3
329,285 + Rv0272c 0.63 -0.9

Or see this region's nucleotide sequence