Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2023c

Experiment: 7H9 with 2-bromo-2-nitro-1,3-propanediol 0.1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2021c and Rv2022c are separated by 8 nucleotidesRv2022c and Rv2023c are separated by 24 nucleotidesRv2023c and Rv2023A are separated by 159 nucleotidesRv2023A and Rv2024c overlap by 34 nucleotides Rv2021c: Rv2021c - Transcriptional regulatory protein, at 2,266,805 to 2,267,110 Rv2021c Rv2022c: Rv2022c - Conserved protein, at 2,267,119 to 2,267,724 Rv2022c Rv2023c: Rv2023c - Hypothetical protein, at 2,267,749 to 2,268,108 Rv2023c Rv2023A: Rv2023A - Hypothetical protein, pseudogene, at 2,268,268 to 2,268,726 Rv2023A Rv2024c: Rv2024c - Conserved hypothetical protein, at 2,268,693 to 2,270,240 Rv2024c Position (kb) 2267 2268 2269Strain fitness (log2 ratio) -2 -1 0 1 2at 2267.078 kb on + strand, within Rv2021cat 2267.079 kb on - strand, within Rv2021cat 2267.079 kb on - strand, within Rv2021cat 2267.096 kb on + strandat 2267.096 kb on + strandat 2267.096 kb on + strandat 2267.097 kb on - strandat 2267.097 kb on - strandat 2267.120 kb on + strandat 2267.121 kb on - strandat 2267.121 kb on - strandat 2267.121 kb on - strandat 2267.216 kb on + strand, within Rv2022cat 2267.244 kb on + strand, within Rv2022cat 2267.418 kb on + strand, within Rv2022cat 2267.438 kb on + strand, within Rv2022cat 2267.439 kb on - strand, within Rv2022cat 2267.515 kb on + strand, within Rv2022cat 2267.515 kb on + strand, within Rv2022cat 2267.516 kb on - strand, within Rv2022cat 2267.546 kb on + strand, within Rv2022cat 2267.558 kb on + strand, within Rv2022cat 2267.580 kb on + strand, within Rv2022cat 2267.641 kb on + strand, within Rv2022cat 2267.641 kb on + strand, within Rv2022cat 2267.641 kb on + strand, within Rv2022cat 2267.642 kb on - strand, within Rv2022cat 2267.674 kb on + strandat 2267.674 kb on + strandat 2267.675 kb on - strandat 2267.684 kb on + strandat 2267.684 kb on + strandat 2267.684 kb on + strandat 2267.871 kb on + strand, within Rv2023cat 2268.032 kb on + strand, within Rv2023cat 2268.032 kb on + strand, within Rv2023cat 2268.032 kb on + strand, within Rv2023cat 2268.154 kb on + strandat 2268.158 kb on + strandat 2268.253 kb on + strandat 2268.254 kb on - strandat 2268.264 kb on + strandat 2268.364 kb on - strand, within Rv2023Aat 2268.487 kb on + strand, within Rv2023Aat 2268.549 kb on - strand, within Rv2023Aat 2268.556 kb on + strand, within Rv2023Aat 2268.594 kb on + strand, within Rv2023Aat 2268.630 kb on - strand, within Rv2023Aat 2268.695 kb on - strandat 2268.695 kb on - strandat 2269.012 kb on + strand, within Rv2024cat 2269.013 kb on - strand, within Rv2024c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with 2-bromo-2-nitro-1,3-propanediol 0.1 mM
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2,267,078 + Rv2021c 0.89 -0.5
2,267,079 - Rv2021c 0.90 -0.0
2,267,079 - Rv2021c 0.90 +0.5
2,267,096 + +1.6
2,267,096 + -0.4
2,267,096 + +0.8
2,267,097 - +0.2
2,267,097 - -0.0
2,267,120 + -0.1
2,267,121 - -0.7
2,267,121 - +0.9
2,267,121 - -0.2
2,267,216 + Rv2022c 0.16 -0.4
2,267,244 + Rv2022c 0.21 +1.2
2,267,418 + Rv2022c 0.49 +1.0
2,267,438 + Rv2022c 0.53 -0.6
2,267,439 - Rv2022c 0.53 -0.4
2,267,515 + Rv2022c 0.65 -0.8
2,267,515 + Rv2022c 0.65 -0.8
2,267,516 - Rv2022c 0.66 +0.1
2,267,546 + Rv2022c 0.70 -0.2
2,267,558 + Rv2022c 0.72 -0.5
2,267,580 + Rv2022c 0.76 -0.6
2,267,641 + Rv2022c 0.86 -0.3
2,267,641 + Rv2022c 0.86 -0.9
2,267,641 + Rv2022c 0.86 -0.4
2,267,642 - Rv2022c 0.86 +1.5
2,267,674 + -1.1
2,267,674 + +1.9
2,267,675 - -0.2
2,267,684 + -1.7
2,267,684 + -0.1
2,267,684 + +1.1
2,267,871 + Rv2023c 0.34 -1.4
2,268,032 + Rv2023c 0.79 -0.6
2,268,032 + Rv2023c 0.79 -1.0
2,268,032 + Rv2023c 0.79 -0.5
2,268,154 + +1.8
2,268,158 + -0.2
2,268,253 + -0.1
2,268,254 - -0.6
2,268,264 + -0.3
2,268,364 - Rv2023A 0.21 -1.3
2,268,487 + Rv2023A 0.48 -1.0
2,268,549 - Rv2023A 0.61 +0.1
2,268,556 + Rv2023A 0.63 -0.6
2,268,594 + Rv2023A 0.71 -2.7
2,268,630 - Rv2023A 0.79 -0.4
2,268,695 - -0.3
2,268,695 - +1.0
2,269,012 + Rv2024c 0.21 +0.5
2,269,013 - Rv2024c 0.21 -0.5

Or see this region's nucleotide sequence