Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0625c

Experiment: 7H9 with 2-bromo-2-nitro-1,3-propanediol 0.1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0622 and Rv0623 are separated by 92 nucleotidesRv0623 and Rv0624 overlap by 1 nucleotidesRv0624 and Rv0625c are separated by 93 nucleotidesRv0625c and Rv0626 are separated by 131 nucleotidesRv0626 and Rv0627 overlap by 4 nucleotidesRv0627 and Rv0628c are separated by 71 nucleotides Rv0622: Rv0622 - Possible membrane protein, at 715,370 to 716,317 Rv0622 Rv0623: Rv0623 - Possible antitoxin VapB30, at 716,410 to 716,664 Rv0623 Rv0624: Rv0624 - Possible toxin VapC30. Contains PIN domain., at 716,664 to 717,059 Rv0624 Rv0625c: Rv0625c - Probable conserved transmembrane protein, at 717,153 to 717,893 Rv0625c Rv0626: Rv0626 - Possible antitoxin VapB5, at 718,025 to 718,285 Rv0626 Rv0627: Rv0627 - Possible toxin VapC5, at 718,282 to 718,689 Rv0627 Rv0628c: Rv0628c - Conserved hypothetical protein, at 718,761 to 719,912 Rv0628c Position (kb) 717 718Strain fitness (log2 ratio) -3 -2 -1 0 1at 716.231 kb on + strandat 716.232 kb on - strandat 716.422 kb on - strandat 716.422 kb on - strandat 716.638 kb on + strand, within Rv0623at 716.639 kb on - strandat 716.797 kb on + strand, within Rv0624at 716.798 kb on - strand, within Rv0624at 716.919 kb on + strand, within Rv0624at 716.920 kb on - strand, within Rv0624at 716.952 kb on + strand, within Rv0624at 716.953 kb on - strand, within Rv0624at 716.970 kb on + strand, within Rv0624at 716.971 kb on - strand, within Rv0624at 717.057 kb on + strandat 717.058 kb on - strandat 717.058 kb on - strandat 717.064 kb on + strandat 717.428 kb on - strand, within Rv0625cat 717.428 kb on - strand, within Rv0625cat 717.718 kb on + strand, within Rv0625cat 717.719 kb on - strand, within Rv0625cat 717.719 kb on - strand, within Rv0625cat 717.802 kb on + strand, within Rv0625cat 717.803 kb on - strand, within Rv0625cat 717.803 kb on - strand, within Rv0625cat 717.813 kb on + strand, within Rv0625cat 717.813 kb on + strand, within Rv0625cat 718.001 kb on + strandat 718.020 kb on + strandat 718.054 kb on + strand, within Rv0626

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with 2-bromo-2-nitro-1,3-propanediol 0.1 mM
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716,231 + +1.6
716,232 - -0.0
716,422 - -0.3
716,422 - -1.1
716,638 + Rv0623 0.89 -0.6
716,639 - +0.1
716,797 + Rv0624 0.34 -0.8
716,798 - Rv0624 0.34 -0.1
716,919 + Rv0624 0.64 -2.9
716,920 - Rv0624 0.65 -0.5
716,952 + Rv0624 0.73 -0.9
716,953 - Rv0624 0.73 -0.3
716,970 + Rv0624 0.77 -0.3
716,971 - Rv0624 0.78 +0.4
717,057 + -0.7
717,058 - -3.5
717,058 - -0.0
717,064 + +0.5
717,428 - Rv0625c 0.37 -0.2
717,428 - Rv0625c 0.37 -0.7
717,718 + Rv0625c 0.76 -0.4
717,719 - Rv0625c 0.76 -1.1
717,719 - Rv0625c 0.76 -0.3
717,802 + Rv0625c 0.88 -0.6
717,803 - Rv0625c 0.88 -1.8
717,803 - Rv0625c 0.88 -0.9
717,813 + Rv0625c 0.89 -0.4
717,813 + Rv0625c 0.89 -1.4
718,001 + -1.2
718,020 + +0.5
718,054 + Rv0626 0.11 +0.2

Or see this region's nucleotide sequence