Experiment: 7H9 with 2-bromo-2-nitro-1,3-propanediol 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv2591 and Rv2592c are separated by 16 nucleotides Rv2592c and Rv2593c overlap by 4 nucleotides Rv2593c and Rv2594c overlap by 4 nucleotides
Rv2591: Rv2591 - PE-PGRS family protein PE_PGRS44, at 2,921,551 to 2,923,182
Rv2591
Rv2592c: Rv2592c - Probable holliday junction DNA helicase RuvB, at 2,923,199 to 2,924,233
Rv2592c
Rv2593c: Rv2593c - Probable holliday junction DNA helicase RuvA, at 2,924,230 to 2,924,820
Rv2593c
Rv2594c: Rv2594c - Probable crossover junction endodeoxyribonuclease RuvC (holliday junction nuclease) (holliday junction resolvase), at 2,924,817 to 2,925,383
Rv2594c
Position (kb)
2923
2924
2925 Strain fitness (log2 ratio)
-1
0
1 at 2922.389 kb on + strand, within Rv2591 at 2922.390 kb on - strand, within Rv2591 at 2922.390 kb on - strand, within Rv2591 at 2922.390 kb on - strand, within Rv2591 at 2922.631 kb on - strand, within Rv2591 at 2922.969 kb on + strand, within Rv2591 at 2922.969 kb on + strand, within Rv2591 at 2922.969 kb on + strand, within Rv2591 at 2922.969 kb on + strand, within Rv2591 at 2922.969 kb on + strand, within Rv2591 at 2922.969 kb on + strand, within Rv2591 at 2922.969 kb on + strand, within Rv2591 at 2923.200 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with 2-bromo-2-nitro-1,3-propanediol 0.1 mM remove 2,922,389 + Rv2591 0.51 -0.2 2,922,390 - Rv2591 0.51 +0.2 2,922,390 - Rv2591 0.51 +0.1 2,922,390 - Rv2591 0.51 -0.2 2,922,631 - Rv2591 0.66 +0.0 2,922,969 + Rv2591 0.87 -0.6 2,922,969 + Rv2591 0.87 +0.8 2,922,969 + Rv2591 0.87 -0.6 2,922,969 + Rv2591 0.87 -0.1 2,922,969 + Rv2591 0.87 +0.8 2,922,969 + Rv2591 0.87 -0.2 2,922,969 + Rv2591 0.87 -1.5 2,923,200 + -0.2
Or see this region's nucleotide sequence