Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2592c

Experiment: 7H9 with 2-bromo-2-nitro-1,3-propanediol 0.1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2591 and Rv2592c are separated by 16 nucleotidesRv2592c and Rv2593c overlap by 4 nucleotidesRv2593c and Rv2594c overlap by 4 nucleotides Rv2591: Rv2591 - PE-PGRS family protein PE_PGRS44, at 2,921,551 to 2,923,182 Rv2591 Rv2592c: Rv2592c - Probable holliday junction DNA helicase RuvB, at 2,923,199 to 2,924,233 Rv2592c Rv2593c: Rv2593c - Probable holliday junction DNA helicase RuvA, at 2,924,230 to 2,924,820 Rv2593c Rv2594c: Rv2594c - Probable crossover junction endodeoxyribonuclease RuvC (holliday junction nuclease) (holliday junction resolvase), at 2,924,817 to 2,925,383 Rv2594c Position (kb) 2923 2924 2925Strain fitness (log2 ratio) -1 0 1at 2922.389 kb on + strand, within Rv2591at 2922.390 kb on - strand, within Rv2591at 2922.390 kb on - strand, within Rv2591at 2922.390 kb on - strand, within Rv2591at 2922.631 kb on - strand, within Rv2591at 2922.969 kb on + strand, within Rv2591at 2922.969 kb on + strand, within Rv2591at 2922.969 kb on + strand, within Rv2591at 2922.969 kb on + strand, within Rv2591at 2922.969 kb on + strand, within Rv2591at 2922.969 kb on + strand, within Rv2591at 2922.969 kb on + strand, within Rv2591at 2923.200 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with 2-bromo-2-nitro-1,3-propanediol 0.1 mM
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2,922,389 + Rv2591 0.51 -0.2
2,922,390 - Rv2591 0.51 +0.2
2,922,390 - Rv2591 0.51 +0.1
2,922,390 - Rv2591 0.51 -0.2
2,922,631 - Rv2591 0.66 +0.0
2,922,969 + Rv2591 0.87 -0.6
2,922,969 + Rv2591 0.87 +0.8
2,922,969 + Rv2591 0.87 -0.6
2,922,969 + Rv2591 0.87 -0.1
2,922,969 + Rv2591 0.87 +0.8
2,922,969 + Rv2591 0.87 -0.2
2,922,969 + Rv2591 0.87 -1.5
2,923,200 + -0.2

Or see this region's nucleotide sequence