Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv1204c

Experiment: 7H9 with 2-bromo-2-nitro-1,3-propanediol 0.1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv1202 and Rv1203c overlap by 4 nucleotidesRv1203c and Rv1204c are separated by 30 nucleotidesRv1204c and Rv1205 are separated by 94 nucleotidesRv1205 and Rv1206 are separated by 49 nucleotides Rv1202: Rv1202 - Probable succinyl-diaminopimelate desuccinylase DapE, at 1,345,260 to 1,346,324 Rv1202 Rv1203c: Rv1203c - Hypothetical protein, at 1,346,321 to 1,346,905 Rv1203c Rv1204c: Rv1204c - Conserved hypothetical protein, at 1,346,936 to 1,348,624 Rv1204c Rv1205: Rv1205 - Conserved hypothetical protein, at 1,348,719 to 1,349,282 Rv1205 Rv1206: Rv1206 - Probable fatty-acid-CoA ligase FadD6 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase), at 1,349,332 to 1,351,125 Rv1206 Position (kb) 1346 1347 1348 1349Strain fitness (log2 ratio) -1 0 1 2at 1346.467 kb on + strand, within Rv1203cat 1346.468 kb on - strand, within Rv1203cat 1346.888 kb on + strandat 1346.889 kb on - strandat 1346.889 kb on - strandat 1346.889 kb on - strandat 1346.892 kb on + strandat 1346.892 kb on + strandat 1346.893 kb on - strandat 1346.938 kb on - strandat 1347.116 kb on + strand, within Rv1204cat 1347.117 kb on - strand, within Rv1204cat 1347.457 kb on - strand, within Rv1204cat 1347.593 kb on - strand, within Rv1204cat 1347.633 kb on - strand, within Rv1204cat 1347.633 kb on - strand, within Rv1204cat 1347.633 kb on - strand, within Rv1204cat 1347.741 kb on + strand, within Rv1204cat 1347.741 kb on + strand, within Rv1204cat 1347.772 kb on + strand, within Rv1204cat 1347.894 kb on + strand, within Rv1204cat 1347.895 kb on - strand, within Rv1204cat 1347.937 kb on - strand, within Rv1204cat 1347.937 kb on - strand, within Rv1204cat 1348.038 kb on + strand, within Rv1204cat 1348.193 kb on + strand, within Rv1204cat 1348.193 kb on + strand, within Rv1204cat 1348.196 kb on - strand, within Rv1204cat 1348.196 kb on - strand, within Rv1204cat 1348.266 kb on + strand, within Rv1204cat 1348.266 kb on + strand, within Rv1204cat 1348.267 kb on - strand, within Rv1204cat 1348.267 kb on - strand, within Rv1204cat 1348.351 kb on + strand, within Rv1204cat 1348.636 kb on - strandat 1348.685 kb on - strandat 1348.706 kb on + strandat 1348.706 kb on + strandat 1348.719 kb on - strandat 1348.719 kb on - strandat 1348.719 kb on - strandat 1348.764 kb on + strandat 1348.798 kb on + strand, within Rv1205at 1348.798 kb on + strand, within Rv1205at 1348.798 kb on + strand, within Rv1205at 1349.021 kb on + strand, within Rv1205at 1349.021 kb on + strand, within Rv1205at 1349.074 kb on + strand, within Rv1205at 1349.140 kb on + strand, within Rv1205at 1349.202 kb on + strand, within Rv1205at 1349.202 kb on + strand, within Rv1205at 1349.203 kb on - strand, within Rv1205at 1349.203 kb on - strand, within Rv1205at 1349.203 kb on - strand, within Rv1205at 1349.236 kb on + strandat 1349.330 kb on - strandat 1349.330 kb on - strandat 1349.330 kb on - strandat 1349.341 kb on + strandat 1349.342 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with 2-bromo-2-nitro-1,3-propanediol 0.1 mM
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1,346,467 + Rv1203c 0.25 -0.3
1,346,468 - Rv1203c 0.25 +0.9
1,346,888 + -0.1
1,346,889 - +0.3
1,346,889 - -0.3
1,346,889 - -1.7
1,346,892 + -0.1
1,346,892 + -0.0
1,346,893 - -0.2
1,346,938 - +1.0
1,347,116 + Rv1204c 0.11 +1.3
1,347,117 - Rv1204c 0.11 -0.1
1,347,457 - Rv1204c 0.31 +0.5
1,347,593 - Rv1204c 0.39 +0.4
1,347,633 - Rv1204c 0.41 +0.5
1,347,633 - Rv1204c 0.41 -0.1
1,347,633 - Rv1204c 0.41 -0.3
1,347,741 + Rv1204c 0.48 -0.0
1,347,741 + Rv1204c 0.48 -0.2
1,347,772 + Rv1204c 0.49 -0.4
1,347,894 + Rv1204c 0.57 +0.1
1,347,895 - Rv1204c 0.57 +0.8
1,347,937 - Rv1204c 0.59 +0.3
1,347,937 - Rv1204c 0.59 -0.1
1,348,038 + Rv1204c 0.65 +0.3
1,348,193 + Rv1204c 0.74 +0.1
1,348,193 + Rv1204c 0.74 +1.0
1,348,196 - Rv1204c 0.75 +0.5
1,348,196 - Rv1204c 0.75 -0.6
1,348,266 + Rv1204c 0.79 +1.1
1,348,266 + Rv1204c 0.79 -0.3
1,348,267 - Rv1204c 0.79 +0.1
1,348,267 - Rv1204c 0.79 +0.1
1,348,351 + Rv1204c 0.84 +1.9
1,348,636 - +0.4
1,348,685 - -0.0
1,348,706 + -0.2
1,348,706 + -0.7
1,348,719 - +0.8
1,348,719 - +0.8
1,348,719 - +0.3
1,348,764 + +1.7
1,348,798 + Rv1205 0.14 -0.1
1,348,798 + Rv1205 0.14 +0.6
1,348,798 + Rv1205 0.14 +0.9
1,349,021 + Rv1205 0.54 +0.5
1,349,021 + Rv1205 0.54 +0.2
1,349,074 + Rv1205 0.63 +1.9
1,349,140 + Rv1205 0.75 +0.8
1,349,202 + Rv1205 0.86 +0.9
1,349,202 + Rv1205 0.86 +0.8
1,349,203 - Rv1205 0.86 +0.9
1,349,203 - Rv1205 0.86 +0.6
1,349,203 - Rv1205 0.86 +0.6
1,349,236 + +0.6
1,349,330 - +0.4
1,349,330 - +0.3
1,349,330 - +0.8
1,349,341 + -1.7
1,349,342 - +1.2

Or see this region's nucleotide sequence