Experiment: 7H9 with 2-bromo-2-nitro-1,3-propanediol 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0585c and Rv0586 are separated by 137 nucleotides Rv0586 and Rv0587 overlap by 4 nucleotides Rv0587 and Rv0588 are separated by 1 nucleotides Rv0588 and Rv0589 are separated by 5 nucleotides
Rv0585c: Rv0585c - Probable conserved integral membrane protein, at 681,885 to 684,272
Rv0585c
Rv0586: Rv0586 - Probable transcriptional regulatory protein Mce2R (GntR-family), at 684,410 to 685,132
Rv0586
Rv0587: Rv0587 - Conserved hypothetical integral membrane protein YrbE2A, at 685,129 to 685,926
Rv0587
Rv0588: Rv0588 - Conserved hypothetical integral membrane protein YrbE2B, at 685,928 to 686,815
Rv0588
Rv0589: Rv0589 - Mce-family protein Mce2A, at 686,821 to 688,035
Rv0589
Position (kb) 685
686 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 684.278 kb on - strand at 684.278 kb on - strand at 684.339 kb on + strand at 684.700 kb on + strand, within Rv0586 at 685.157 kb on - strand at 685.217 kb on + strand, within Rv0587 at 685.218 kb on - strand, within Rv0587 at 685.359 kb on + strand, within Rv0587 at 686.544 kb on - strand, within Rv0588 at 686.573 kb on + strand, within Rv0588 at 686.663 kb on + strand, within Rv0588 at 686.667 kb on - strand, within Rv0588 at 686.673 kb on - strand, within Rv0588 at 686.781 kb on - strand at 686.863 kb on + strand at 686.864 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with 2-bromo-2-nitro-1,3-propanediol 0.1 mM remove 684,278 - -0.5 684,278 - +0.2 684,339 + -0.3 684,700 + Rv0586 0.40 -0.6 685,157 - +0.5 685,217 + Rv0587 0.11 -2.1 685,218 - Rv0587 0.11 -0.0 685,359 + Rv0587 0.29 +2.5 686,544 - Rv0588 0.69 -1.1 686,573 + Rv0588 0.73 -0.6 686,663 + Rv0588 0.83 -0.2 686,667 - Rv0588 0.83 +0.6 686,673 - Rv0588 0.84 +2.8 686,781 - +1.3 686,863 + -0.7 686,864 - -1.5
Or see this region's nucleotide sequence