Experiment: 7H9 with 2-bromo-2-nitro-1,3-propanediol 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0404 and Rv0405 overlap by 4 nucleotides Rv0405 and Rv0406c overlap by 53 nucleotides Rv0406c and Rv0407 are separated by 77 nucleotides
Rv0404: Rv0404 - Fatty-acid-AMP ligase FadD30 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase), at 483,977 to 485,734
Rv0404
Rv0405: Rv0405 - Probable membrane bound polyketide synthase Pks6, at 485,731 to 489,939
Rv0405
Rv0406c: Rv0406c - Beta lactamase like protein, at 489,887 to 490,705
Rv0406c
Rv0407: Rv0407 - F420-dependent glucose-6-phosphate dehydrogenase Fgd1, at 490,783 to 491,793
Rv0407
Position (kb) 485
486
487
488
489
490 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 485.041 kb on + strand, within Rv0404 at 485.341 kb on - strand, within Rv0404 at 485.384 kb on - strand, within Rv0404 at 486.044 kb on + strand at 486.045 kb on - strand at 486.560 kb on + strand, within Rv0405 at 486.561 kb on - strand, within Rv0405 at 486.561 kb on - strand, within Rv0405 at 486.984 kb on - strand, within Rv0405 at 487.050 kb on + strand, within Rv0405 at 487.193 kb on + strand, within Rv0405 at 487.194 kb on - strand, within Rv0405 at 487.194 kb on - strand, within Rv0405 at 487.194 kb on - strand, within Rv0405 at 487.429 kb on + strand, within Rv0405 at 487.429 kb on + strand, within Rv0405 at 487.430 kb on - strand, within Rv0405 at 488.282 kb on - strand, within Rv0405 at 488.329 kb on + strand, within Rv0405 at 488.330 kb on - strand, within Rv0405 at 488.379 kb on + strand, within Rv0405 at 488.380 kb on - strand, within Rv0405 at 488.380 kb on - strand, within Rv0405 at 488.380 kb on - strand, within Rv0405 at 488.380 kb on - strand, within Rv0405 at 488.621 kb on - strand, within Rv0405 at 488.621 kb on - strand, within Rv0405 at 488.798 kb on + strand, within Rv0405 at 488.799 kb on - strand, within Rv0405 at 488.799 kb on - strand, within Rv0405 at 488.799 kb on - strand, within Rv0405 at 488.799 kb on - strand, within Rv0405 at 488.903 kb on + strand, within Rv0405 at 488.903 kb on + strand, within Rv0405 at 488.903 kb on + strand, within Rv0405 at 488.903 kb on + strand, within Rv0405 at 488.904 kb on - strand, within Rv0405 at 488.908 kb on - strand, within Rv0405 at 488.915 kb on + strand, within Rv0405 at 488.916 kb on - strand, within Rv0405 at 488.933 kb on + strand, within Rv0405 at 488.934 kb on - strand, within Rv0405 at 488.989 kb on + strand, within Rv0405 at 489.005 kb on + strand, within Rv0405 at 489.532 kb on - strand at 489.535 kb on + strand at 489.535 kb on + strand at 489.536 kb on - strand at 489.680 kb on - strand at 489.937 kb on + strand at 490.064 kb on + strand, within Rv0406c at 490.064 kb on + strand, within Rv0406c at 490.065 kb on - strand, within Rv0406c at 490.143 kb on + strand, within Rv0406c at 490.144 kb on - strand, within Rv0406c at 490.144 kb on - strand, within Rv0406c at 490.282 kb on + strand, within Rv0406c at 490.370 kb on + strand, within Rv0406c at 490.390 kb on - strand, within Rv0406c at 490.488 kb on + strand, within Rv0406c at 490.488 kb on + strand, within Rv0406c at 490.758 kb on + strand at 490.759 kb on - strand at 490.765 kb on + strand at 490.766 kb on - strand at 490.766 kb on - strand at 490.771 kb on + strand at 490.771 kb on + strand at 490.772 kb on - strand at 490.772 kb on - strand at 490.772 kb on - strand at 490.799 kb on + strand at 490.804 kb on + strand at 490.804 kb on + strand at 490.805 kb on - strand at 490.805 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with 2-bromo-2-nitro-1,3-propanediol 0.1 mM remove 485,041 + Rv0404 0.61 -0.6 485,341 - Rv0404 0.78 +0.5 485,384 - Rv0404 0.80 -0.5 486,044 + -0.1 486,045 - -0.2 486,560 + Rv0405 0.20 -0.4 486,561 - Rv0405 0.20 +1.8 486,561 - Rv0405 0.20 +0.0 486,984 - Rv0405 0.30 -0.5 487,050 + Rv0405 0.31 -0.4 487,193 + Rv0405 0.35 +0.8 487,194 - Rv0405 0.35 +0.2 487,194 - Rv0405 0.35 +1.8 487,194 - Rv0405 0.35 -1.9 487,429 + Rv0405 0.40 +0.8 487,429 + Rv0405 0.40 -0.2 487,430 - Rv0405 0.40 -0.1 488,282 - Rv0405 0.61 +0.8 488,329 + Rv0405 0.62 +0.2 488,330 - Rv0405 0.62 +0.1 488,379 + Rv0405 0.63 +1.9 488,380 - Rv0405 0.63 +0.9 488,380 - Rv0405 0.63 -0.6 488,380 - Rv0405 0.63 -0.6 488,380 - Rv0405 0.63 -0.4 488,621 - Rv0405 0.69 -1.4 488,621 - Rv0405 0.69 -0.8 488,798 + Rv0405 0.73 -1.1 488,799 - Rv0405 0.73 +0.2 488,799 - Rv0405 0.73 -0.9 488,799 - Rv0405 0.73 -0.0 488,799 - Rv0405 0.73 -0.5 488,903 + Rv0405 0.75 +1.8 488,903 + Rv0405 0.75 -0.6 488,903 + Rv0405 0.75 +0.1 488,903 + Rv0405 0.75 -0.3 488,904 - Rv0405 0.75 -0.7 488,908 - Rv0405 0.75 -0.8 488,915 + Rv0405 0.76 +0.1 488,916 - Rv0405 0.76 -1.0 488,933 + Rv0405 0.76 -1.0 488,934 - Rv0405 0.76 -0.1 488,989 + Rv0405 0.77 -1.2 489,005 + Rv0405 0.78 +0.2 489,532 - -0.1 489,535 + +1.2 489,535 + -0.1 489,536 - +0.2 489,680 - -0.8 489,937 + -0.9 490,064 + Rv0406c 0.22 -0.2 490,064 + Rv0406c 0.22 -0.0 490,065 - Rv0406c 0.22 -1.3 490,143 + Rv0406c 0.31 +0.2 490,144 - Rv0406c 0.31 -0.7 490,144 - Rv0406c 0.31 +0.0 490,282 + Rv0406c 0.48 +0.3 490,370 + Rv0406c 0.59 -0.4 490,390 - Rv0406c 0.61 -0.9 490,488 + Rv0406c 0.73 +0.9 490,488 + Rv0406c 0.73 -0.2 490,758 + -0.1 490,759 - +0.5 490,765 + -1.6 490,766 - -1.3 490,766 - -1.3 490,771 + +1.8 490,771 + -0.3 490,772 - -0.1 490,772 - -0.7 490,772 - -0.6 490,799 + +1.2 490,804 + -3.9 490,804 + +0.8 490,805 - -3.6 490,805 - -0.8
Or see this region's nucleotide sequence