Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2474c

Experiment: 7H9 with Benzethonium chloride 0.068 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2472 and Rv2473 are separated by 2 nucleotidesRv2473 and Rv2474c are separated by 31 nucleotidesRv2474c and Rv2475c are separated by 5 nucleotidesRv2475c and Rv2476c overlap by 4 nucleotides Rv2472: Rv2472 - Conserved hypothetical protein, at 2,775,272 to 2,775,565 Rv2472 Rv2473: Rv2473 - Possible alanine and proline rich membrane protein, at 2,775,568 to 2,776,284 Rv2473 Rv2474c: Rv2474c - Conserved hypothetical protein, at 2,776,316 to 2,776,969 Rv2474c Rv2475c: Rv2475c - Conserved protein, at 2,776,975 to 2,777,391 Rv2475c Rv2476c: Rv2476c - Probable NAD-dependent glutamate dehydrogenase Gdh (NAD-Gdh) (NAD-dependent glutamic dehydrogenase), at 2,777,388 to 2,782,262 Rv2476c Position (kb) 2776 2777Strain fitness (log2 ratio) -2 -1 0 1at 2775.549 kb on + strandat 2775.549 kb on + strandat 2775.563 kb on + strandat 2775.697 kb on - strand, within Rv2473at 2775.697 kb on - strand, within Rv2473at 2775.715 kb on + strand, within Rv2473at 2775.831 kb on - strand, within Rv2473at 2775.851 kb on + strand, within Rv2473at 2775.852 kb on - strand, within Rv2473at 2776.176 kb on - strand, within Rv2473at 2776.195 kb on + strand, within Rv2473at 2776.196 kb on - strand, within Rv2473at 2776.196 kb on - strand, within Rv2473at 2776.223 kb on + strandat 2776.224 kb on - strandat 2776.641 kb on + strand, within Rv2474cat 2776.977 kb on - strandat 2777.000 kb on + strandat 2777.001 kb on - strandat 2777.211 kb on - strand, within Rv2475cat 2777.274 kb on + strand, within Rv2475cat 2777.336 kb on + strand, within Rv2475cat 2777.336 kb on + strand, within Rv2475cat 2777.336 kb on + strand, within Rv2475cat 2777.336 kb on + strand, within Rv2475cat 2777.611 kb on - strandat 2777.735 kb on - strandat 2777.830 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Benzethonium chloride 0.068 mM
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2,775,549 + -0.4
2,775,549 + -0.4
2,775,563 + -0.6
2,775,697 - Rv2473 0.18 +1.0
2,775,697 - Rv2473 0.18 -0.3
2,775,715 + Rv2473 0.21 -0.3
2,775,831 - Rv2473 0.37 -0.5
2,775,851 + Rv2473 0.39 -0.6
2,775,852 - Rv2473 0.40 -0.8
2,776,176 - Rv2473 0.85 -0.2
2,776,195 + Rv2473 0.87 +0.6
2,776,196 - Rv2473 0.88 -0.5
2,776,196 - Rv2473 0.88 +0.0
2,776,223 + -0.1
2,776,224 - +0.1
2,776,641 + Rv2474c 0.50 -2.1
2,776,977 - -0.2
2,777,000 + +0.5
2,777,001 - +0.6
2,777,211 - Rv2475c 0.57 -0.8
2,777,274 + Rv2475c 0.72 -2.2
2,777,336 + Rv2475c 0.87 -1.4
2,777,336 + Rv2475c 0.87 -1.8
2,777,336 + Rv2475c 0.87 +0.3
2,777,336 + Rv2475c 0.87 -1.6
2,777,611 - -0.6
2,777,735 - -0.3
2,777,830 + -0.3

Or see this region's nucleotide sequence