Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3528c

Experiment: 7H9 with Spectinomycin 0.029 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3526 and Rv3527 are separated by 5 nucleotidesRv3527 and Rv3528c are separated by 424 nucleotidesRv3528c and Rv3529c are separated by 691 nucleotides Rv3526: Rv3526 - Oxygenase component of 3-ketosteroid-9-alpha-hydroxylase KshA, at 3,962,439 to 3,963,599 Rv3526 Rv3527: Rv3527 - Hypothetical protein, at 3,963,605 to 3,964,054 Rv3527 Rv3528c: Rv3528c - Unknown protein, at 3,964,479 to 3,965,192 Rv3528c Rv3529c: Rv3529c - Conserved hypothetical protein, at 3,965,884 to 3,967,038 Rv3529c Position (kb) 3964 3965 3966Strain fitness (log2 ratio) -2 -1 0 1 2at 3963.807 kb on - strand, within Rv3527at 3964.026 kb on - strandat 3964.152 kb on - strandat 3964.152 kb on - strandat 3964.152 kb on - strandat 3964.152 kb on - strandat 3964.152 kb on - strandat 3964.276 kb on - strandat 3964.793 kb on + strand, within Rv3528cat 3964.826 kb on - strand, within Rv3528cat 3964.829 kb on + strand, within Rv3528cat 3964.838 kb on + strand, within Rv3528cat 3964.839 kb on - strand, within Rv3528cat 3964.946 kb on + strand, within Rv3528cat 3965.014 kb on - strand, within Rv3528cat 3965.204 kb on + strandat 3965.237 kb on - strandat 3965.443 kb on + strandat 3965.559 kb on - strandat 3965.838 kb on + strandat 3966.048 kb on + strand, within Rv3529cat 3966.049 kb on - strand, within Rv3529c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Spectinomycin 0.029 mM
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3,963,807 - Rv3527 0.45 +0.5
3,964,026 - -1.1
3,964,152 - -1.8
3,964,152 - +1.0
3,964,152 - -0.7
3,964,152 - -0.7
3,964,152 - -0.2
3,964,276 - -0.3
3,964,793 + Rv3528c 0.44 -0.2
3,964,826 - Rv3528c 0.49 -0.7
3,964,829 + Rv3528c 0.49 -0.6
3,964,838 + Rv3528c 0.50 +0.1
3,964,839 - Rv3528c 0.50 +0.9
3,964,946 + Rv3528c 0.65 -0.4
3,965,014 - Rv3528c 0.75 -1.2
3,965,204 + +2.5
3,965,237 - -1.3
3,965,443 + +0.9
3,965,559 - +0.4
3,965,838 + -0.4
3,966,048 + Rv3529c 0.14 +1.9
3,966,049 - Rv3529c 0.14 -0.6

Or see this region's nucleotide sequence