Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3337

Experiment: 7H9 with Spectinomycin 0.029 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3336c and Rv3337 are separated by 24 nucleotidesRv3337 and Rv3338 overlap by 139 nucleotidesRv3338 and Rv3339c are separated by 66 nucleotides Rv3336c: Rv3336c - Probable tryptophanyl-tRNA synthetase TrpS (tryptophan--tRNA ligase) (TRPRS) (tryptophan translase), at 3,722,621 to 3,723,631 Rv3336c Rv3337: Rv3337 - Conserved hypothetical protein, at 3,723,656 to 3,724,042 Rv3337 Rv3338: Rv3338 - Conserved hypothetical protein, at 3,723,904 to 3,724,548 Rv3338 Rv3339c: Rv3339c - Probable isocitrate dehydrogenase [NADP] Icd1 (oxalosuccinate decarboxylase) (IDH) (NADP+-specific ICDH) (IDP), at 3,724,615 to 3,725,844 Rv3339c Position (kb) 3723 3724 3725Strain fitness (log2 ratio) -1 0 1at 3723.715 kb on + strand, within Rv3337at 3723.727 kb on + strand, within Rv3337at 3723.728 kb on - strand, within Rv3337at 3723.769 kb on - strand, within Rv3337at 3723.774 kb on + strand, within Rv3337at 3723.774 kb on + strand, within Rv3337at 3723.774 kb on + strand, within Rv3337at 3723.775 kb on - strand, within Rv3337at 3723.849 kb on + strand, within Rv3337at 3723.849 kb on + strand, within Rv3337at 3723.849 kb on + strand, within Rv3337at 3723.850 kb on - strand, within Rv3337at 3723.973 kb on - strandat 3723.973 kb on - strandat 3724.105 kb on - strand, within Rv3338at 3724.277 kb on - strand, within Rv3338at 3724.547 kb on - strandat 3724.547 kb on - strandat 3724.816 kb on + strand, within Rv3339cat 3724.817 kb on - strand, within Rv3339cat 3724.881 kb on - strand, within Rv3339cat 3724.881 kb on - strand, within Rv3339cat 3724.890 kb on - strand, within Rv3339cat 3724.982 kb on + strand, within Rv3339cat 3724.982 kb on + strand, within Rv3339cat 3725.021 kb on + strand, within Rv3339c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Spectinomycin 0.029 mM
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3,723,715 + Rv3337 0.15 +0.9
3,723,727 + Rv3337 0.18 -0.5
3,723,728 - Rv3337 0.19 +1.1
3,723,769 - Rv3337 0.29 +1.6
3,723,774 + Rv3337 0.30 -0.5
3,723,774 + Rv3337 0.30 +0.6
3,723,774 + Rv3337 0.30 -0.3
3,723,775 - Rv3337 0.31 +0.8
3,723,849 + Rv3337 0.50 -0.2
3,723,849 + Rv3337 0.50 -0.2
3,723,849 + Rv3337 0.50 -1.6
3,723,850 - Rv3337 0.50 +1.5
3,723,973 - -0.2
3,723,973 - -0.5
3,724,105 - Rv3338 0.31 +1.0
3,724,277 - Rv3338 0.58 -0.4
3,724,547 - +0.2
3,724,547 - -0.8
3,724,816 + Rv3339c 0.16 -0.4
3,724,817 - Rv3339c 0.16 +0.3
3,724,881 - Rv3339c 0.22 -0.3
3,724,881 - Rv3339c 0.22 -0.7
3,724,890 - Rv3339c 0.22 -0.3
3,724,982 + Rv3339c 0.30 -0.1
3,724,982 + Rv3339c 0.30 -0.7
3,725,021 + Rv3339c 0.33 +1.5

Or see this region's nucleotide sequence