Experiment: 7H9 with Spectinomycin 0.029 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0568 and Rv0569 are separated by 134 nucleotides Rv0569 and Rv0570 are separated by 25 nucleotides
Rv0568: Rv0568 - Possible cytochrome P450 135B1 Cyp135B1, at 659,450 to 660,868
Rv0568
Rv0569: Rv0569 - Conserved protein, at 661,003 to 661,269
Rv0569
Rv0570: Rv0570 - Probable ribonucleoside-diphosphate reductase (large subunit) NrdZ (ribonucleotide reductase), at 661,295 to 663,373
Rv0570
Position (kb)
661
662 Strain fitness (log2 ratio)
-2
-1
0
1 at 660.086 kb on + strand, within Rv0568 at 660.086 kb on + strand, within Rv0568 at 660.087 kb on - strand, within Rv0568 at 660.359 kb on + strand, within Rv0568 at 660.360 kb on - strand, within Rv0568 at 660.407 kb on + strand, within Rv0568 at 660.408 kb on - strand, within Rv0568 at 660.518 kb on + strand, within Rv0568 at 660.519 kb on - strand, within Rv0568 at 660.717 kb on + strand, within Rv0568 at 660.718 kb on - strand, within Rv0568 at 661.049 kb on + strand, within Rv0569 at 661.077 kb on + strand, within Rv0569 at 661.078 kb on - strand, within Rv0569 at 661.111 kb on + strand, within Rv0569 at 661.111 kb on + strand, within Rv0569 at 661.112 kb on - strand, within Rv0569 at 661.267 kb on + strand at 661.267 kb on + strand at 661.267 kb on + strand at 661.544 kb on + strand, within Rv0570 at 661.545 kb on - strand, within Rv0570 at 661.614 kb on + strand, within Rv0570 at 661.614 kb on + strand, within Rv0570 at 662.051 kb on + strand, within Rv0570 at 662.052 kb on - strand, within Rv0570 at 662.244 kb on + strand, within Rv0570
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Spectinomycin 0.029 mM remove 660,086 + Rv0568 0.45 -0.5 660,086 + Rv0568 0.45 -0.1 660,087 - Rv0568 0.45 +0.1 660,359 + Rv0568 0.64 -0.3 660,360 - Rv0568 0.64 +0.3 660,407 + Rv0568 0.67 -0.3 660,408 - Rv0568 0.68 -0.6 660,518 + Rv0568 0.75 +1.1 660,519 - Rv0568 0.75 -1.0 660,717 + Rv0568 0.89 -1.1 660,718 - Rv0568 0.89 +0.2 661,049 + Rv0569 0.17 +0.3 661,077 + Rv0569 0.28 -0.1 661,078 - Rv0569 0.28 -1.3 661,111 + Rv0569 0.40 -2.5 661,111 + Rv0569 0.40 -0.9 661,112 - Rv0569 0.41 +1.5 661,267 + -0.4 661,267 + -1.0 661,267 + +0.1 661,544 + Rv0570 0.12 +0.3 661,545 - Rv0570 0.12 +0.3 661,614 + Rv0570 0.15 -1.5 661,614 + Rv0570 0.15 -0.1 662,051 + Rv0570 0.36 -0.4 662,052 - Rv0570 0.36 +0.1 662,244 + Rv0570 0.46 -0.2
Or see this region's nucleotide sequence