Experiment: 7H9 with Spectinomycin 0.029 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0468 and Rv0469 are separated by 132 nucleotides Rv0469 and Rv0470c are separated by 99 nucleotides
Rv0468: Rv0468 - 3-hydroxybutyryl-CoA dehydrogenase FadB2 (beta-hydroxybutyryl-CoA dehydrogenase) (BHBD), at 558,895 to 559,755
Rv0468
Rv0469: Rv0469 - Possible mycolic acid synthase UmaA, at 559,888 to 560,748
Rv0469
Rv0470c: Rv0470c - Mycolic acid synthase PcaA (cyclopropane synthase), at 560,848 to 561,711
Rv0470c
Position (kb) 559
560
561 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 559.140 kb on - strand, within Rv0468 at 559.255 kb on - strand, within Rv0468 at 559.572 kb on - strand, within Rv0468 at 559.699 kb on - strand at 559.750 kb on + strand at 559.750 kb on + strand at 559.750 kb on + strand at 559.751 kb on - strand at 559.760 kb on + strand at 559.761 kb on - strand at 559.761 kb on - strand at 559.761 kb on - strand at 559.853 kb on - strand at 559.910 kb on - strand at 559.931 kb on - strand at 559.981 kb on + strand, within Rv0469 at 559.982 kb on - strand, within Rv0469 at 560.140 kb on + strand, within Rv0469 at 560.140 kb on + strand, within Rv0469 at 560.141 kb on - strand, within Rv0469 at 560.160 kb on + strand, within Rv0469 at 560.161 kb on - strand, within Rv0469 at 560.188 kb on + strand, within Rv0469 at 560.354 kb on + strand, within Rv0469 at 560.591 kb on + strand, within Rv0469 at 560.620 kb on - strand, within Rv0469 at 560.668 kb on + strand at 560.669 kb on - strand at 560.747 kb on - strand at 560.747 kb on - strand at 560.927 kb on + strand at 560.928 kb on - strand at 560.936 kb on + strand, within Rv0470c at 560.937 kb on - strand, within Rv0470c at 561.017 kb on + strand, within Rv0470c at 561.272 kb on + strand, within Rv0470c at 561.615 kb on - strand, within Rv0470c
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Spectinomycin 0.029 mM remove 559,140 - Rv0468 0.28 -1.0 559,255 - Rv0468 0.42 -0.2 559,572 - Rv0468 0.79 +1.2 559,699 - -0.7 559,750 + -0.6 559,750 + +2.3 559,750 + -0.4 559,751 - -0.0 559,760 + +0.1 559,761 - -1.1 559,761 - -1.5 559,761 - +1.3 559,853 - +0.1 559,910 - -0.0 559,931 - -0.3 559,981 + Rv0469 0.11 +0.3 559,982 - Rv0469 0.11 +0.2 560,140 + Rv0469 0.29 +1.6 560,140 + Rv0469 0.29 +0.6 560,141 - Rv0469 0.29 -0.2 560,160 + Rv0469 0.32 +1.2 560,161 - Rv0469 0.32 +0.6 560,188 + Rv0469 0.35 -1.4 560,354 + Rv0469 0.54 -0.2 560,591 + Rv0469 0.82 +0.4 560,620 - Rv0469 0.85 -1.7 560,668 + -0.3 560,669 - +1.9 560,747 - -0.6 560,747 - -0.2 560,927 + -2.7 560,928 - +0.0 560,936 + Rv0470c 0.10 -0.1 560,937 - Rv0470c 0.10 -0.9 561,017 + Rv0470c 0.20 -0.1 561,272 + Rv0470c 0.49 -3.1 561,615 - Rv0470c 0.89 +0.1
Or see this region's nucleotide sequence