Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0212c

Experiment: 7H9 with Spectinomycin 0.029 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0211 and Rv0212c are separated by 66 nucleotidesRv0212c and Rv0213c overlap by 4 nucleotides Rv0211: Rv0211 - Probable iron-regulated phosphoenolpyruvate carboxykinase [GTP] PckA (phosphoenolpyruvate carboxylase) (PEPCK)(pep carboxykinase), at 251,782 to 253,602 Rv0211 Rv0212c: Rv0212c - Possible transcriptional regulatory protein NadR (probably AsnC-family), at 253,669 to 254,640 Rv0212c Rv0213c: Rv0213c - Possible methyltransferase (methylase), at 254,637 to 255,950 Rv0213c Position (kb) 253 254 255Strain fitness (log2 ratio) -1 0 1 2at 253.617 kb on + strandat 253.617 kb on + strandat 253.618 kb on - strandat 253.618 kb on - strandat 253.618 kb on - strandat 253.891 kb on + strand, within Rv0212cat 253.892 kb on - strand, within Rv0212cat 253.892 kb on - strand, within Rv0212cat 254.051 kb on - strand, within Rv0212cat 254.334 kb on - strand, within Rv0212cat 254.585 kb on + strandat 254.755 kb on - strandat 254.854 kb on + strand, within Rv0213cat 254.854 kb on + strand, within Rv0213cat 254.854 kb on + strand, within Rv0213cat 254.854 kb on + strand, within Rv0213cat 254.910 kb on - strand, within Rv0213cat 254.910 kb on - strand, within Rv0213cat 254.911 kb on + strand, within Rv0213cat 254.978 kb on + strand, within Rv0213cat 254.978 kb on + strand, within Rv0213cat 254.979 kb on - strand, within Rv0213cat 255.089 kb on - strand, within Rv0213cat 255.272 kb on + strand, within Rv0213cat 255.273 kb on - strand, within Rv0213cat 255.295 kb on - strand, within Rv0213cat 255.311 kb on - strand, within Rv0213cat 255.381 kb on + strand, within Rv0213cat 255.381 kb on + strand, within Rv0213cat 255.429 kb on - strand, within Rv0213cat 255.573 kb on + strand, within Rv0213cat 255.573 kb on + strand, within Rv0213c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Spectinomycin 0.029 mM
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253,617 + -1.0
253,617 + -0.9
253,618 - -0.1
253,618 - -0.3
253,618 - +1.2
253,891 + Rv0212c 0.23 -0.2
253,892 - Rv0212c 0.23 -0.4
253,892 - Rv0212c 0.23 -0.3
254,051 - Rv0212c 0.39 -0.1
254,334 - Rv0212c 0.68 +1.5
254,585 + +0.1
254,755 - +0.9
254,854 + Rv0213c 0.17 -0.0
254,854 + Rv0213c 0.17 -0.7
254,854 + Rv0213c 0.17 +0.9
254,854 + Rv0213c 0.17 +1.9
254,910 - Rv0213c 0.21 -0.8
254,910 - Rv0213c 0.21 +0.4
254,911 + Rv0213c 0.21 +0.6
254,978 + Rv0213c 0.26 +0.4
254,978 + Rv0213c 0.26 -0.0
254,979 - Rv0213c 0.26 +0.5
255,089 - Rv0213c 0.34 +0.2
255,272 + Rv0213c 0.48 +1.5
255,273 - Rv0213c 0.48 -0.1
255,295 - Rv0213c 0.50 -0.4
255,311 - Rv0213c 0.51 -0.5
255,381 + Rv0213c 0.57 -1.0
255,381 + Rv0213c 0.57 -0.3
255,429 - Rv0213c 0.60 +1.9
255,573 + Rv0213c 0.71 -0.6
255,573 + Rv0213c 0.71 -0.1

Or see this region's nucleotide sequence