Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0078

Experiment: 7H9 with Spectinomycin 0.029 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0076c and Rv0077c are separated by 63 nucleotidesRv0077c and Rv0078 are separated by 61 nucleotidesRv0078 and Rv0078A are separated by 74 nucleotidesRv0078A and Rv0078B overlap by 4 nucleotides Rv0076c: Rv0076c - Probable membrane protein, at 85,183 to 85,572 Rv0076c Rv0077c: Rv0077c - Probable oxidoreductase, at 85,636 to 86,466 Rv0077c Rv0078: Rv0078 - Probable transcriptional regulatory protein, at 86,528 to 87,133 Rv0078 Rv0078A: Rv0078A - Hypothetical protein, at 87,208 to 87,801 Rv0078A Rv0078B: Rv0078B - Conserved protein, at 87,798 to 88,004 Rv0078B Position (kb) 86 87 88Strain fitness (log2 ratio) -2 -1 0 1at 85.576 kb on - strandat 85.576 kb on - strandat 85.721 kb on - strand, within Rv0077cat 86.104 kb on - strand, within Rv0077cat 86.359 kb on + strand, within Rv0077cat 86.359 kb on + strand, within Rv0077cat 86.360 kb on - strand, within Rv0077cat 86.378 kb on + strand, within Rv0077cat 86.430 kb on - strandat 86.436 kb on + strandat 86.448 kb on + strandat 86.528 kb on - strandat 86.682 kb on - strand, within Rv0078at 86.836 kb on - strand, within Rv0078at 86.925 kb on - strand, within Rv0078at 87.125 kb on - strandat 87.276 kb on + strand, within Rv0078Aat 87.289 kb on - strand, within Rv0078Aat 87.343 kb on + strand, within Rv0078Aat 87.605 kb on + strand, within Rv0078Aat 87.606 kb on - strand, within Rv0078Aat 87.632 kb on - strand, within Rv0078Aat 87.955 kb on + strand, within Rv0078Bat 87.955 kb on + strand, within Rv0078Bat 87.955 kb on + strand, within Rv0078Bat 87.955 kb on + strand, within Rv0078Bat 87.955 kb on + strand, within Rv0078B

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Spectinomycin 0.029 mM
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85,576 - -0.1
85,576 - -0.1
85,721 - Rv0077c 0.10 +1.4
86,104 - Rv0077c 0.56 +0.5
86,359 + Rv0077c 0.87 +0.6
86,359 + Rv0077c 0.87 +0.1
86,360 - Rv0077c 0.87 -0.6
86,378 + Rv0077c 0.89 +0.1
86,430 - -0.0
86,436 + +1.2
86,448 + -0.3
86,528 - -1.0
86,682 - Rv0078 0.25 -1.8
86,836 - Rv0078 0.51 -2.3
86,925 - Rv0078 0.66 -0.9
87,125 - -0.5
87,276 + Rv0078A 0.11 +0.1
87,289 - Rv0078A 0.14 -1.7
87,343 + Rv0078A 0.23 -0.7
87,605 + Rv0078A 0.67 -0.4
87,606 - Rv0078A 0.67 -0.3
87,632 - Rv0078A 0.71 -0.9
87,955 + Rv0078B 0.76 -0.5
87,955 + Rv0078B 0.76 +0.5
87,955 + Rv0078B 0.76 -0.7
87,955 + Rv0078B 0.76 -1.0
87,955 + Rv0078B 0.76 -0.3

Or see this region's nucleotide sequence