Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3659c

Experiment: 7H9 with Chlorpromazine 0.054 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3657c and Rv3658c are separated by 23 nucleotidesRv3658c and Rv3659c overlap by 4 nucleotidesRv3659c and Rv3660c are separated by 101 nucleotides Rv3657c: Rv3657c - Possible conserved alanine rich membrane protein, at 4,095,540 to 4,096,115 Rv3657c Rv3658c: Rv3658c - Probable conserved transmembrane protein, at 4,096,139 to 4,096,939 Rv3658c Rv3659c: Rv3659c - Conserved hypothetical protein, at 4,096,936 to 4,097,994 Rv3659c Rv3660c: Rv3660c - Conserved hypothetical protein, at 4,098,096 to 4,099,148 Rv3660c Position (kb) 4096 4097 4098Strain fitness (log2 ratio) -2 -1 0 1at 4096.023 kb on + strand, within Rv3657cat 4096.310 kb on + strand, within Rv3658cat 4096.711 kb on - strand, within Rv3658cat 4096.930 kb on + strandat 4097.215 kb on + strand, within Rv3659cat 4097.216 kb on - strand, within Rv3659cat 4097.216 kb on - strand, within Rv3659cat 4097.216 kb on - strand, within Rv3659cat 4097.722 kb on + strand, within Rv3659cat 4097.722 kb on + strand, within Rv3659cat 4098.249 kb on + strand, within Rv3660cat 4098.531 kb on + strand, within Rv3660cat 4098.538 kb on + strand, within Rv3660cat 4098.627 kb on + strand, within Rv3660cat 4098.628 kb on - strand, within Rv3660c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Chlorpromazine 0.054 mM
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4,096,023 + Rv3657c 0.84 -0.5
4,096,310 + Rv3658c 0.21 +0.6
4,096,711 - Rv3658c 0.71 -1.0
4,096,930 + -0.1
4,097,215 + Rv3659c 0.26 -0.8
4,097,216 - Rv3659c 0.26 +0.3
4,097,216 - Rv3659c 0.26 -1.1
4,097,216 - Rv3659c 0.26 -2.0
4,097,722 + Rv3659c 0.74 -0.2
4,097,722 + Rv3659c 0.74 -1.5
4,098,249 + Rv3660c 0.15 -0.3
4,098,531 + Rv3660c 0.41 +0.6
4,098,538 + Rv3660c 0.42 -0.0
4,098,627 + Rv3660c 0.50 -0.4
4,098,628 - Rv3660c 0.51 +0.2

Or see this region's nucleotide sequence