Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2133c

Experiment: 7H9 with Chlorpromazine 0.054 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2131c and Rv2132 are separated by 90 nucleotidesRv2132 and Rv2133c are separated by 209 nucleotidesRv2133c and Rv2134c are separated by 10 nucleotidesRv2134c and Rv2135c are separated by 63 nucleotides Rv2131c: Rv2131c - Monophosphatase CysQ, at 2,392,517 to 2,393,320 Rv2131c Rv2132: Rv2132 - Conserved hypothetical protein, at 2,393,411 to 2,393,641 Rv2132 Rv2133c: Rv2133c - Conserved hypothetical protein, at 2,393,851 to 2,394,639 Rv2133c Rv2134c: Rv2134c - Conserved protein, at 2,394,650 to 2,395,237 Rv2134c Rv2135c: Rv2135c - Conserved protein, at 2,395,301 to 2,396,011 Rv2135c Position (kb) 2393 2394 2395Strain fitness (log2 ratio) -3 -2 -1 0 1at 2393.564 kb on + strand, within Rv2132at 2393.565 kb on - strand, within Rv2132at 2393.565 kb on - strand, within Rv2132at 2393.588 kb on + strand, within Rv2132at 2393.639 kb on + strandat 2393.852 kb on + strandat 2393.852 kb on + strandat 2394.120 kb on - strand, within Rv2133cat 2394.120 kb on - strand, within Rv2133cat 2394.177 kb on + strand, within Rv2133cat 2394.178 kb on - strand, within Rv2133cat 2394.178 kb on - strand, within Rv2133cat 2394.178 kb on - strand, within Rv2133cat 2394.178 kb on - strand, within Rv2133cat 2394.178 kb on - strand, within Rv2133cat 2394.258 kb on - strand, within Rv2133cat 2394.379 kb on + strand, within Rv2133cat 2394.379 kb on + strand, within Rv2133cat 2394.379 kb on + strand, within Rv2133cat 2394.380 kb on - strand, within Rv2133cat 2394.380 kb on - strand, within Rv2133cat 2394.388 kb on + strand, within Rv2133cat 2394.389 kb on - strand, within Rv2133cat 2394.533 kb on + strand, within Rv2133cat 2394.831 kb on - strand, within Rv2134cat 2394.831 kb on - strand, within Rv2134cat 2395.151 kb on + strand, within Rv2134cat 2395.151 kb on + strand, within Rv2134cat 2395.151 kb on + strand, within Rv2134cat 2395.152 kb on - strand, within Rv2134cat 2395.155 kb on + strand, within Rv2134cat 2395.156 kb on - strand, within Rv2134cat 2395.156 kb on - strand, within Rv2134cat 2395.217 kb on + strandat 2395.218 kb on - strandat 2395.218 kb on - strandat 2395.218 kb on - strandat 2395.302 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Chlorpromazine 0.054 mM
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2,393,564 + Rv2132 0.66 -0.2
2,393,565 - Rv2132 0.67 -0.1
2,393,565 - Rv2132 0.67 -0.5
2,393,588 + Rv2132 0.77 +0.2
2,393,639 + +0.6
2,393,852 + -2.8
2,393,852 + +0.3
2,394,120 - Rv2133c 0.34 +1.3
2,394,120 - Rv2133c 0.34 -0.3
2,394,177 + Rv2133c 0.41 +0.5
2,394,178 - Rv2133c 0.41 -0.7
2,394,178 - Rv2133c 0.41 -0.2
2,394,178 - Rv2133c 0.41 +1.6
2,394,178 - Rv2133c 0.41 -0.5
2,394,178 - Rv2133c 0.41 -0.0
2,394,258 - Rv2133c 0.52 +0.4
2,394,379 + Rv2133c 0.67 -0.0
2,394,379 + Rv2133c 0.67 -0.2
2,394,379 + Rv2133c 0.67 +1.6
2,394,380 - Rv2133c 0.67 -0.3
2,394,380 - Rv2133c 0.67 -0.2
2,394,388 + Rv2133c 0.68 -0.5
2,394,389 - Rv2133c 0.68 +0.0
2,394,533 + Rv2133c 0.86 +0.4
2,394,831 - Rv2134c 0.31 -0.5
2,394,831 - Rv2134c 0.31 -0.7
2,395,151 + Rv2134c 0.85 +0.1
2,395,151 + Rv2134c 0.85 -0.7
2,395,151 + Rv2134c 0.85 -0.2
2,395,152 - Rv2134c 0.85 -0.9
2,395,155 + Rv2134c 0.86 +0.3
2,395,156 - Rv2134c 0.86 +0.2
2,395,156 - Rv2134c 0.86 -0.4
2,395,217 + +0.3
2,395,218 - -0.7
2,395,218 - -1.0
2,395,218 - +0.1
2,395,302 + -0.3

Or see this region's nucleotide sequence